Sensitive immunodetection through impedance measurements onto gold functionalized electrodes.

abstract：:Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

journal_title：Applied biochemistry and biotechnology

authors： Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

更新日期：2011-06-01 00:00:00

abstract：:A study was carried out to select the conditions for cultivation of Kluyveromyces marxianus CDBBL 278 in solid-state culture (SSC) using polyurethane foam (PUF) as an inert support. PUF was impregnated with culture media containing lactose (50 g/L) as the carbon and energy source. Evaluation of culture parameters duri...

journal_title：Applied biochemistry and biotechnology

authors： Tovar-Castro L,García-Garibay M,Saucedo-Castañeda G

更新日期：2008-12-01 00:00:00

abstract：:Valdecoxib is a new anti-inflammatory drug that is highly selective for inhibition of the inducible form of cyclooxygenase (COX-2). In the present study, biotransformation of valdecoxib was investigated in cell cultures of five medicinal plants, viz., Catharanthus roseus, Azadirachta indica, Capsicum annuum, Ervatamia...

journal_title：Applied biochemistry and biotechnology

authors： Molmoori V,Srisailam K,Ciddi V

更新日期：2008-03-01 00:00:00

abstract：:Fuel ethanol can be produced from softwood through hydrolysis in an enzymatic process. Prior to enzymatic hydrolysis of the softwood, pretreatment is necessary. In this study, two-step steam pretreatment employing dilute H2SO4 impregnation in the first step and SO2 impregnation in the second step, to improve the overa...

journal_title：Applied biochemistry and biotechnology

authors： Söderström J,Pilcher L,Galbe M,Zacchi G

更新日期：2003-04-01 00:00:00

abstract：:An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

journal_title：Applied biochemistry and biotechnology

authors： He X,Miao Y,Jiang X,Xu Z,Ouyang P

更新日期：2010-04-01 00:00:00

abstract：:In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

journal_title：Applied biochemistry and biotechnology

authors： Silva-Jiménez H,Zazueta-Sandoval R

更新日期：2005-04-01 00:00:00

abstract：:Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

journal_title：Applied biochemistry and biotechnology

authors： Xia C,Wei W,Hu B

更新日期：2014-04-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:A protein-engineered beta-lactamase, constructed by site-directed mutagenesis in Escherichia coli (E104M/G238S), and having broadened specificity, was able to degrade cephalosporins of first, second, and third generations. Manipulations of culture conditions allowed an increase in beta-lactamase specific activity by u...

journal_title：Applied biochemistry and biotechnology

authors： García JL,Nuñez CJ,González EG,Osuna J,Soberón X,Galindo E

更新日期：1998-05-01 00:00:00

abstract：:The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...

journal_title：Applied biochemistry and biotechnology

authors： Chi B,Li H,Zhang J,Wei P,Gao J,Liu R

更新日期：2019-04-01 00:00:00

abstract：:In this work, the catalytic conversion of natural polymer chitosan to 5-HMF under low temperature hydrothermal condition was investigated. The highest 5-HMF yield of 12.1 wt% was obtained under the following conditions: 174 °C reaction temperature, 2.2 % catalyst amount, and 36.9 min reaction time. In the effect of co...

journal_title：Applied biochemistry and biotechnology

authors： Lee SB,Jeong GT

更新日期：2015-06-01 00:00:00

abstract：:Small RNAs influence the gene expression at the post-transcriptional level by guiding messenger RNA (mRNA) cleavage, translational repression, and chromatin modifications. In addition to model plants, the microRNAs (miRNAs) have been identified in different crop species. In this work, we developed a specific pipeline ...

journal_title：Applied biochemistry and biotechnology

authors： Chaves SS,Fernandes-Brum CN,Silva GF,Ferrara-Barbosa BC,Paiva LV,Nogueira FT,Cardoso TC,Amaral LR,de Souza Gomes M,Chalfun-Junior A

更新日期：2015-10-01 00:00:00

abstract：:A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively...

journal_title：Applied biochemistry and biotechnology

authors： Takuma S,Nakashima N,Tantirungkij M,Kinoshita S,Okada H,Seki T,Yoshida T

更新日期：1991-04-01 00:00:00

abstract：:The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

journal_title：Applied biochemistry and biotechnology

authors： MacCallum JD,Hefford MA,Omar S,Beauregard M

更新日期：1997-04-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:In the present work, protective effect of tetrahydrocurcumin (THC) against oxidative damages in cadmium (Cd)-induced toxicity in rats was evaluated. Cd is an important environmental and industrial toxicant that affects almost all the organs, especially liver. Liver is the major organ responsible for the metabolism and...

journal_title：Applied biochemistry and biotechnology

authors： Ramakrishnan R,Elangovan P,Pari L

更新日期：2017-09-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:The potential of dilute-acid prehydrolysis as a pretreatment method for sugarcane bagasse, rice hulls, peanut shells, and cassava stalks was investigated. The prehydrolysis was performed at 122 degrees C during 20, 40, or 60 min using 2% H(2)SO(4) at a solid-to-liquid ratio of 1:10. Sugar formation increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Martin C,Alriksson B,Sjöde A,Nilvebrant NO,Jönsson LJ

更新日期：2007-04-01 00:00:00

abstract：:An improved procedure has been developed for efficient somatic embryogenesis in Anethum graveolens. Green friable embryogenic callus was obtained from hypocotyl segments on medium augmented with 2,4-dichlorophenoxyacetic acid (2,4-D). The highest embryogenic callus induction frequency of 87 % was obtained on Murashige...

journal_title：Applied biochemistry and biotechnology

authors： Dhir R,Shekhawat GS,Alam A

更新日期：2014-08-01 00:00:00

abstract：:Currently, fumaric acid is produced by catalytic isomerization of maleic acid in aqueous solutions at low pH. Being petroleum based, requiring catalyst, and producing vast amounts of by-products and wastewater, the production of fumaric acid from renewable resources by a "green" process is increasingly attractive. In ...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Song J,Tan T,Liu L

更新日期：2015-03-01 00:00:00

abstract：:Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

journal_title：Applied biochemistry and biotechnology

authors： Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

更新日期：2012-07-01 00:00:00

abstract：:The increase in waste disposal and energy costs has provided an incentive to convert carbohydrate-rich food waste streams into fuel. For example, dining halls and restaurants discard foods that require tipping fees for removal. An effective use of food waste may be the enzymatic hydrolysis of the waste to simple sugar...

journal_title：Applied biochemistry and biotechnology

authors： Davis RA

更新日期：2008-03-01 00:00:00

abstract：:The autoimmune repertoire is well known from previous studies to be capable of producing catalytic antibodies directed to self-antigens. In the present study, we explored the ability of 26 monoclonal light chains (L chains) from multiple myeloma patients to cleave radiolabeled gp120, a foreign protein. One L chain wit...

journal_title：Applied biochemistry and biotechnology

authors： Paul S,Kalaga RS,Gololobov G,Brenneman D

更新日期：2000-01-01 00:00:00

abstract：:Internal air circulation affects the temperature field distribution in a gas double-dynamic solid-state fermentation bioreactor (GDSFB). To enhance heat transfer through strengthening internal air circulation in a GDSFB, we put an air distribution plate (ADP) into the bioreactor and studied the effects of forced inter...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Qin L,Li H

更新日期：2014-02-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:A highly sensitive, selective, reversible, and reusable glucose sensor is developed by using molecularly imprinted polymer-based artificial receptors onto interdigital transducer. Sensor receptors were synthesized through bulk imprinting technology by using styrene as monomer, ethylene glycol dimethacrylate (EGDMA) as...

journal_title：Applied biochemistry and biotechnology

authors： Asghar N,Mustafa G,Yasinzai M,Al-Soud YA,Lieberzeit PA,Latif U

更新日期：2019-12-01 00:00:00

abstract：:Arabidopsis transcription factor APETALA2 (AP2) controls multiple aspects of plant growth and development, including seed development, stem cell maintenance, and specification of floral organ identity. Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species, degenerate RT-PCR and...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Jiang J,Yang Y,Li P,Chen S,Zhang Z

更新日期：2012-09-01 00:00:00

abstract：:Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best...

journal_title：Applied biochemistry and biotechnology

authors： Kumar A,Singh S,Poddar P,Prabhune A,Pundle A

更新日期：2009-06-01 00:00:00

abstract：:Reactive oxygen species (ROS) in plants, arising from various environmental stresses, impair the thiol-contained proteins that are susceptible to irregular oxidative formation of disulfide bonds, which might be alleviated by a relatively specific modifier called protein disulfide isomerase (PDI). From our previous dat...

journal_title：Applied biochemistry and biotechnology

authors： Wang H,Zou Z,Gong M

更新日期：2015-05-01 00:00:00

abstract：:Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...

journal_title：Applied biochemistry and biotechnology

authors： Shah A,Priyadarshini M,Khan MS,Aatif M,Amin F,Bano B

更新日期：2013-10-01 00:00:00