Influence of forced internal air circulation on airflow distribution and heat transfer in a gas double-dynamic solid-state fermentation bioreactor.

abstract：:Silver nanoparticles (AgNPs) were synthesised using Kalopanax septemlobus plant leaf extracts. UV-visible spectrophotometric, Fourier-transform infrared, electron dispersive X-ray spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses confirmed synthesis of AgNPs. TEM micr...

journal_title：Applied biochemistry and biotechnology

authors： Salunke BK,Sawant SS,Kim BS

更新日期：2014-09-01 00:00:00

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35degreesC. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the so...

journal_title：Applied biochemistry and biotechnology

authors： Huang WC,Ramey DE,Yang ST

更新日期：2004-04-01 00:00:00

abstract：:Since the two reports published in 1986 by the laboratories of R. Lerner and P. G. Schultz, it has been clearly established that antibodies may be induced to act as catalysts in numerous chemical reactions. In all cases, catalytic antibodies were elicited using a substrate-based approach. In the present article, we pr...

journal_title：Applied biochemistry and biotechnology

authors： Friboulet A,Izadyar L,Avalle B,Roseto A,Thomas D

更新日期：1994-05-01 00:00:00

abstract：:An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination ...

journal_title：Applied biochemistry and biotechnology

authors： Fatima N,Ahmad N,Ahmad I,Anis M

更新日期：2015-09-01 00:00:00

abstract：:Growth kinetics and red pigment production of Monascus purpureus CCT 3802 was studied. A reproducible inoculum with extremely dispersed hyphae for bioreactor runs was obtained through a two-step cultivation in a shaker. First, the spores were cultivated in a complex medium rendering a suspension of vegetative cells. I...

journal_title：Applied biochemistry and biotechnology

authors： Pereira DG,Kilikian BV

更新日期：2001-04-01 00:00:00

abstract：:Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol ferment...

journal_title：Applied biochemistry and biotechnology

authors： Sun L,Wang L,Chen H

更新日期：2020-10-01 00:00:00

abstract：:An anaerobic sequencing batch biofilm reactor (AnSBBR-total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 °C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m(-3) day(-1)), which were varied...

journal_title：Applied biochemistry and biotechnology

authors： Manssouri M,Rodrigues JA,Ratusznei SM,Zaiat M

更新日期：2013-12-01 00:00:00

abstract：:A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been d...

journal_title：Applied biochemistry and biotechnology

authors： Wu Q,Chen CX,Du LL,Lin XF

更新日期：2010-04-01 00:00:00

abstract：:The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs proliferation has yet been reported. In the present study, the feas...

journal_title：Applied biochemistry and biotechnology

authors： Yu Y,Li K,Bao C,Liu T,Jin Y,Ren H,Yun W

更新日期：2009-10-01 00:00:00

abstract：:The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently o...

journal_title：Applied biochemistry and biotechnology

authors： Muthuraman P

更新日期：2014-06-01 00:00:00

abstract：:Antifreeze proteins are a group of proteins that allow organisms to survive in subzero environments. These proteins possess thermal hysteresis and ice recrystallization inhibition activities. In the present study, we demonstrated the efficiency of a recombinant antifreeze protein from the Arctic yeast Leucosporidium s...

journal_title：Applied biochemistry and biotechnology

authors： Koh HY,Lee JH,Han SJ,Park H,Lee SG

更新日期：2015-01-01 00:00:00

abstract：:Neoadjuvant chemotherapy (NACT) is known to be beneficial for patients with locally advanced breast cancer. However, there is still no unified standard on the evaluation of NACT. To identify the potential markers related to NACT sensitivity of breast cancer, in the present study, we examined the protein spectrum of br...

journal_title：Applied biochemistry and biotechnology

authors： Zhang K,Yuan K,Wu H,Li Q,Wang Y,Chen S,Zhang L,Gu H,Fu R

更新日期：2012-02-01 00:00:00

abstract：:The ability of Xanthomonas campestris to convert glucose and xylose to xanthan and the structure of xanthan derived from the glucose/xylose mixture media are important when the lignocelluloses hydrolysate was used in xanthan production. In this paper, the features related to xanthan fermentation in the glucose/xylose ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Z,Chen H

更新日期：2010-03-01 00:00:00

abstract：:Binding of phenyl derivative of pyranocoumarin (PDP) modulated activity of fungal endopolygalacturonase in silico. Induced fit docking study of PDP with endopolygalacturonase (1HG8) showed a bifurcated hydrogen bond interaction with the protein at Lys 244 with a docking score of -3.6 and glide energy of -37.30 kcal/mo...

journal_title：Applied biochemistry and biotechnology

authors： Sangeetha S,Sarada DV

更新日期：2015-01-01 00:00:00

abstract：:Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In the present study, the kinetic assay in air-saturated solutions and the kinetic behavior of PO from Pieris rapae (Lepidoptera) larvae ...

journal_title：Applied biochemistry and biotechnology

authors： Xue CB,Luo WC,Jiang L,Xie XY,Xiao T,Yan L

更新日期：2007-11-01 00:00:00

abstract：:Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

journal_title：Applied biochemistry and biotechnology

authors： Hassani L,Nourozi R

更新日期：2014-04-01 00:00:00

abstract：:Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

journal_title：Applied biochemistry and biotechnology

authors： Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

更新日期：2011-06-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:Thermomucor indicae-seudaticae, a glucoamylase-producing thermophilic mould, was mutagenised using nitrous acid and gamma ((60)Co) irradiation in a sequential manner to isolate deregulated mutants for enhanced production of glucoamylase. The mutants were isolated on Emerson YpSs agar containing a non-metabolisable glu...

journal_title：Applied biochemistry and biotechnology

authors： Kumar P,Satyanarayana T

更新日期：2009-07-01 00:00:00

abstract：:Prevalence of heavy metals in the living environment causes chemical stress and reactive oxygen species (ROS) formation in Phanerochaete chrysosporium (P. chrysosporium). However, the mechanisms involved in ROS defense are still under investigation. In the present study, we evaluated the effect of lead- and cadmium-in...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zeng G,Chen G,Yan M,Chen A,Du J,Huang J,Yi B,Zhou Y,He X,He Y

更新日期：2015-02-01 00:00:00

abstract：:More than two decades ago, a group of research engineers and applied scientists with interest in energy applications of bioprocessing initiated an annual symposium series ultimately entitled "Biotechnology for Fuels and Chemicals." The Department of Energy, several of the national laboratories, and various industrial ...

journal_title：Applied biochemistry and biotechnology

authors： Scott CD

更新日期：2004-04-01 00:00:00

abstract：:One-stage autoheated thermophilic aerobic digestion (ATAD) can stabilize sludge to meet class A standard. In this study, batch experiments were conducted to investigate the characteristics of oxidation-reduction potential (ORP), volatile fatty acids (VFAs), soluble chemical oxygen demand (SCOD), and nutrients at diffe...

journal_title：Applied biochemistry and biotechnology

authors： Cheng J,Kong F,Zhu J,Wu X

更新日期：2015-01-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00

abstract：:Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...

journal_title：Applied biochemistry and biotechnology

authors： Lei Y,Yonggang L

更新日期：2012-09-01 00:00:00

abstract：:In this paper we concentrate our attention on the stability and transient behavior of the isothermal system (CSTR) with a substrate-inhibited enzyme reaction producing hydrogen ions. Our investigation covers the region of multiple steady states uncovered previously (1) (ordinary hysteresis and isola). We investigate t...

journal_title：Applied biochemistry and biotechnology

authors： Elnashaie SS,Elrifaie MA,Ibrahim G,Badra G

更新日期：1983-12-01 00:00:00

abstract：:Two protocols for functionalization of glass supports with hexaethylene glycol (HEG)-linked oligonucleotides were developed. The first method (standard amidite protocol) made use of the 2-cyanoethyl-phosphoramidite derivative of 4,4'-dimethoxytrityl-protected HEG. This was first coupled to the support by standard soli...

journal_title：Applied biochemistry and biotechnology

authors： Sojka B,Piunno PAE,Wust CC,Krull UJ

更新日期：2000-10-01 00:00:00

abstract：:In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

journal_title：Applied biochemistry and biotechnology

authors： Silva-Jiménez H,Zazueta-Sandoval R

更新日期：2005-04-01 00:00:00

abstract：:We screened about a hundred microorganisms (including unidentified yeasts, fungi, and bacteria) for their ability to produce xylanolytic enzymes. About 40 of them were hemicellulolytic; among these, we selected Penicillium canescens 10-10c for detailed study because of its ability to produce an interesting enzymatic c...

journal_title：Applied biochemistry and biotechnology

authors： Gaspar A,Cosson T,Roques C,Thonart P

更新日期：1997-07-01 00:00:00

abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...

journal_title：Applied biochemistry and biotechnology

authors： Lawford HG,Rousseau JD

更新日期：2002-04-01 00:00:00