Study on the production of a xylanolytic complex from Penicillium canescens 10-10c.

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:Ornithine carbamoyltransferase folding/unfolding is a complex and not completely understood process. Our experimental results suggest that ornithine carbamoyltransferase interacts in a completely different way with urea and guanidine hydrochloride. In fact, we noticed that, increasing concentration from 0.0 to 8.0 M o...

journal_title：Applied biochemistry and biotechnology

authors： Barreca D,Laganà G,Ficarra S,Tellone E,Leuzzi U,Galtieri A,Bellocco E

更新日期：2014-01-01 00:00:00

abstract：:The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

journal_title：Applied biochemistry and biotechnology

authors： Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

更新日期：2013-01-01 00:00:00

abstract：:Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

journal_title：Applied biochemistry and biotechnology

authors： Rath D,Kumar S,Panda S

更新日期：2019-04-01 00:00:00

abstract：:In order to detect low levels of enzyme activity, specifically glucose oxidase, in biological samples, an immunoenzymatic assay was developed since currently available methods could not be used because of either their lack of sensitivity or the conditions prevailing in our samples: turbidity of the medium, presence of...

journal_title：Applied biochemistry and biotechnology

authors： de Bellefontaine C,Josse F,Domurado M,Domurado D

更新日期：1994-08-01 00:00:00

abstract：:This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced...

journal_title：Applied biochemistry and biotechnology

authors： Reis P,Miller R,Leser M,Watzke H

更新日期：2009-09-01 00:00:00

abstract：:This study aimed to evaluate the influence of the culture medium supplementation with mineral ions, focusing on the growth of Streptococcus zooepidemicus as well as on the production and average molecular weight (MW) of hyaluronic acid (HA). The ions were investigated in terms of individual absence from the totally su...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Eguchi SY,Santana MH

更新日期：2010-12-01 00:00:00

abstract：:Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of...

journal_title：Applied biochemistry and biotechnology

authors： Sukhumsirichart W,Deesukon W,Kawakami T,Matsumoto S,Seesom W,Sakamoto T

更新日期：2014-01-01 00:00:00

abstract：:The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y,...

journal_title：Applied biochemistry and biotechnology

authors： Wang M,Yuan W,Hale A

更新日期：2016-03-01 00:00:00

abstract：:This study investigated methane production in an anaerobic sequencing batch biofilm reactor (AnSBBR) by co-digesting sugarcane vinasse and cheese whey. The assessment was based on the influence of feed strategy, interaction between cycle time and influent concentration, applied volumetric organic load (OLRA), and temp...

journal_title：Applied biochemistry and biotechnology

authors： Sousa SP,Lovato G,Albanez R,Ratusznei SM,Rodrigues JAD

更新日期：2019-11-01 00:00:00

abstract：:Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

journal_title：Applied biochemistry and biotechnology

authors： Ramya LN,Doble M,Rekha VP,Pulicherla KK

更新日期：2012-08-01 00:00:00

abstract：:In this study, benzene, toluene, and xylenes (BTX) removal from contaminated water by physical, chemical, and biological processes was studied. Results showed that air sparging in polluted water can reduce monoaromatic compounds from 140,000 to about 5 microg/l in only 1 h process with a gassing rate of 0.33 VVM. This...

journal_title：Applied biochemistry and biotechnology

authors： Farhadian M,Duchez D,Vachelard C,Larroche C

更新日期：2008-12-01 00:00:00

abstract：:The effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on pH and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus Pleurotus ostreatus was determined. The relat...

journal_title：Applied biochemistry and biotechnology

authors： Ramos C,Morais H,Forgács E,Cserháti T

更新日期：2003-07-01 00:00:00

abstract：:Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...

journal_title：Applied biochemistry and biotechnology

authors： Hinge V,Patil H,Nadaf A

更新日期：2016-02-01 00:00:00

abstract：:MCM-41 and MCM-48 with niobium were successfully synthesized using 1-tetradecyl-3-methylimidazolium chloride ([C14MI]Cl) as a structure-directing agent. The best Si/Nb molar ratio was chosen (Si/Nb = 20) and the CALB enzyme was immobilized in situ in the synthesized Nb-MCM. SEM micrographs showed the formation of very...

journal_title：Applied biochemistry and biotechnology

authors： Bordin I,de Aguiar Pedott V,Demaman Oro CE,Junges A,Dallago RM,Mignoni ML

更新日期：2021-01-06 00:00:00

abstract：:A new method without any special devices for direct transformation of linear gene cassettes was developed. Its feasibility was verified through 5'-fluorescent dye (fluorescein isothiocyanate, FITC)-labeled fluorescent tracing and transient expression of a gus reporter gene. Minimal linear gene cassettes, containing ne...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YQ,Yang J,Xu FP,An LJ,Liu JF,Chen ZW

更新日期：2009-12-01 00:00:00

abstract：:Maltodextrins and hydrolysates of rice and corn flour of varying dextrose equivalents (DE) have been used as a carbon source for alpha-amylase enzyme production by Bacillus sp. TCRDC-25A. The rate and total enzyme production was higher in maltodextrin media than in cornstarch. The enzyme production increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Bajpai P,Bajpai PK

更新日期：1991-11-01 00:00:00

abstract：:Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

journal_title：Applied biochemistry and biotechnology

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2005-03-01 00:00:00

abstract：:Lupulones, naturally produced by glandular trichomes of hop (Humulus lupulus), are prenylated phloroglucinol derivatives that contribute the bitter flavor of beer and demonstrate antimicrobial and anticancer activities. It is appealing to develop microbial cell factories such that lupulones may be produced via ferment...

journal_title：Applied biochemistry and biotechnology

authors： Guo X,Shen H,Liu Y,Wang Q,Wang X,Peng C,Liu W,Zhao ZK

更新日期：2019-07-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the ...

journal_title：Applied biochemistry and biotechnology

authors： Denisova MN,Makarova EI,Pavlov IN,Budaeva VV,Sakovich GV

更新日期：2016-03-01 00:00:00

abstract：:To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensa...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Yao M,Fang X,Yang Y,Cheng X

更新日期：2015-11-01 00:00:00

abstract：:Silver nanoparticles (AgNPs), exhibiting a broad size range and morphologies with highly reactive facets, which are widely applicable in real-life but not fully verified for biosafety and ecotoxicity, were subjected to report transcriptome profile in yeast Saccharomyces cerevisiae. A large number of genes accounted fo...

journal_title：Applied biochemistry and biotechnology

authors： Niazi JH,Sang BI,Kim YS,Gu MB

更新日期：2011-08-01 00:00:00

abstract：:An integrated process of supercritical methanol (SCM) and sodium methoxide catalyst was developed to produce fatty acid methyl esters (FAMEs) via continuous esterification from crude biodiesel. The crude biodiesel with high free fatty acid (FFA) content must be refined to reduce the acid value (AV) for meeting the qua...

journal_title：Applied biochemistry and biotechnology

authors： Zeng D,Li R,Feng M,Fang T

更新日期：2014-10-01 00:00:00

abstract：:Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [poly(HEMA-GMA)] cryogel was synthesized by cryopolymerization technique at frozen temperature. Iminodiacetic acid (IDA) was then attached covalently to the cryogel as a chelating agent. Then, poly(HEMA-GMA)-IDA cryogel was chelated with Ni(II) ions and this n...

journal_title：Applied biochemistry and biotechnology

authors： Uygun M,Akduman B,Akgöl S,Denizli A

更新日期：2013-08-01 00:00:00

abstract：:The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

journal_title：Applied biochemistry and biotechnology

authors： MacCallum JD,Hefford MA,Omar S,Beauregard M

更新日期：1997-04-01 00:00:00

abstract：:The gene encoding a thermostable β-D-xylosidase (GbtXyl43B) from Geobacillus thermoleovorans IT-08 was cloned in pET30a and expressed in Escherichia coli; additionally, characterization and kinetic analysis of GbtXyl43B were carried out. The gene product was purified to apparent homogeneity showing M r of 72 by sodium...

journal_title：Applied biochemistry and biotechnology

authors： Ratnadewi AA,Fanani M,Kurniasih SD,Sakka M,Wasito EB,Sakka K,Nurachman Z,Puspaningsih NN

更新日期：2013-08-01 00:00:00

abstract：:In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

journal_title：Applied biochemistry and biotechnology

authors： Silva-Jiménez H,Zazueta-Sandoval R

更新日期：2005-04-01 00:00:00

abstract：:The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene...

journal_title：Applied biochemistry and biotechnology

authors： Sturesson S,Tjerneld F,Johansson G

更新日期：1990-12-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00