BTX removal from polluted water through bioleaching processes.

abstract：:Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natu...

journal_title：Applied biochemistry and biotechnology

authors： Pedrolli DB,Gomes E,Monti R,Carmona EC

更新日期：2008-02-01 00:00:00

abstract：:Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented fo...

journal_title：Applied biochemistry and biotechnology

authors： Rudolf A,Galbe M,Lidén G

更新日期：2004-04-01 00:00:00

abstract：:A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) follow...

journal_title：Applied biochemistry and biotechnology

authors： Santi-Gadelha T,Rocha BA,Oliveira CC,Aragão KS,Marinho ES,Gadelha CA,Toyama MH,Pinto VP,Nagano CS,Delatorre P,Martins JL,Galvani FR,Sampaio AH,Debray H,Cavada BS

更新日期：2008-07-01 00:00:00

abstract：:Microalgae have tremendous potential as a renewable feedstock for the production of liquid transportation fuels. In natural waters, the importance of physical associations and biochemical interactions between microalgae and bacteria is generally well appreciated, but the significance of these interactions to algal bio...

journal_title：Applied biochemistry and biotechnology

authors： Bagwell CE,Piskorska M,Soule T,Petelos A,Yeager CM

更新日期：2014-08-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...

journal_title：Applied biochemistry and biotechnology

authors： Yoo CG,Nghiem NP,Hicks KB,Kim TH

更新日期：2013-04-01 00:00:00

abstract：:Studying biofilm formation and influence of the matrix composition was heavy because only old and long methods were employed up to now: confocal microscopy, fluorescent chemical markers, and/or dying techniques. In this context, an innovative tool, the BioFilm Ring Test, was here employed to explore the role of exopol...

journal_title：Applied biochemistry and biotechnology

authors： Badel S,Laroche C,Gardarin C,Bernardi T,Michaud P

更新日期：2008-12-01 00:00:00

abstract：:Operational practice of high-rate anaerobic bioreactors such as upflow anaerobic sludge bed (UASB) reactors is generally based on maximization of the biomass concentration and, in the case of more than one reactor compartment, operation in parallel. In this article, a modeling approach is used to postulate that the tr...

journal_title：Applied biochemistry and biotechnology

authors： Kleerebezem R

更新日期：2003-04-01 00:00:00

abstract：:Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Yang D,Wu K,Chen S,Jiang J,Zhang Z

更新日期：2012-02-01 00:00:00

abstract：:Carboxypeptidase N-Sepharose was prepared by covalent immobilization of purified human plasma carboxypeptidase N. More than 98% of the carboxypeptidase N was immobilized; 42% of the applied activity can be detected on the support. The column has excellent capabilities to quantitatively remove carboxy-terminal basic am...

journal_title：Applied biochemistry and biotechnology

authors： Wang W,Hendriks DF,Scharpé SL

更新日期：1994-02-01 00:00:00

abstract：:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

journal_title：Applied biochemistry and biotechnology

authors： Nakanishi K,Ikebukuro K,Karube I

更新日期：1996-08-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:The increasing death rates related to anaplastic lymphoma kinase (ALK)-positive lung cancer culminated in a significant interest in the discovery of novel inhibitors for ALK. In the present research work, pharmacophore-based 3D QSAR modeling and virtual screening strategy have been carried out to address these issues....

journal_title：Applied biochemistry and biotechnology

authors： James N,Shanthi V,Ramanathan K

更新日期：2018-05-01 00:00:00

abstract：:Strain DN002 isolated from petroleum-contaminated soil was identified as Achromobacter xylosoxidans based on morphological and biochemical properties and 16S rRNA phylogeny, and investigated for its potential to utilize numerous polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene and pyrene as sole carbon and...

journal_title：Applied biochemistry and biotechnology

authors： Ma YL,Lu W,Wan LL,Luo N

更新日期：2015-02-01 00:00:00

abstract：:Penicillin acylase of E. coli NCIM 2400 has been purified to homogeneity using a combination of hydrophobic interaction chromatography and DEAE-cellulose treatment. A variety of substituted matrices were synthesized using D- or DL-phenylglycine, norleucine, ampicillin, or amoxycillin as ligands, all of which retained ...

journal_title：Applied biochemistry and biotechnology

authors： Mahajan PB,Borkar PS

更新日期：1984-10-01 00:00:00

abstract：:Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum t...

journal_title：Applied biochemistry and biotechnology

authors： Adibzadeh N,Aminzadeh S,Jamili S,Karkhane AA,Farrokhi N

更新日期：2014-05-01 00:00:00

abstract：:Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...

journal_title：Applied biochemistry and biotechnology

authors： Brumm P,Mead D,Boyum J,Drinkwater C,Deneke J,Gowda K,Stevenson D,Weimer P

更新日期：2011-03-01 00:00:00

abstract：:Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of ...

journal_title：Applied biochemistry and biotechnology

authors： Jia J,Chen H,Wu B,Cui F,Fang H,Wang H,Ni Z

更新日期：2019-01-01 00:00:00

abstract：:Alpha-lactalbumin hydrolysate is of significant interest, due to its potential application as a source of bioactive peptides in nutraceutical and pharmaceutical domains. This study was focused on the cholecystokinin (CCK) family compounds which are small peptides involved in the satiety control. The action of chymotry...

journal_title：Applied biochemistry and biotechnology

authors： Catiau L,Delval-Dubois V,Guillochon D,Nedjar-Arroume N

更新日期：2011-11-01 00:00:00

abstract：:One-stage autoheated thermophilic aerobic digestion (ATAD) can stabilize sludge to meet class A standard. In this study, batch experiments were conducted to investigate the characteristics of oxidation-reduction potential (ORP), volatile fatty acids (VFAs), soluble chemical oxygen demand (SCOD), and nutrients at diffe...

journal_title：Applied biochemistry and biotechnology

authors： Cheng J,Kong F,Zhu J,Wu X

更新日期：2015-01-01 00:00:00

abstract：:A new lipase from seeds of Pachira aquatica was purified to homogeneity by SDS-PAGE obtaining an enzyme with a molecular weight of approximately 55 kDa. The purified lipase exhibited maximum activity at 40 degrees C and pH 8.0, for an incubation time of 90 min. Concerning temperature stability, at the range from 4 to ...

journal_title：Applied biochemistry and biotechnology

authors： Polizelli PP,Facchini FD,Cabral H,Bonilla-Rodriguez GO

更新日期：2008-09-01 00:00:00

abstract：:A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

journal_title：Applied biochemistry and biotechnology

authors： Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

更新日期：2013-05-01 00:00:00

abstract：:The increased additive amount of adjuncts in the raw materials of Chinese beer requires the usage of protease to release more water-soluble proteins. Here, a metallo-neutral protease suited for brewing industry was purified from Bacillus amyloliquefaciens SYB-001. A 5.6-fold purification of the neutral protease was ac...

journal_title：Applied biochemistry and biotechnology

authors： Wang J,Xu A,Wan Y,Li Q

更新日期：2013-08-01 00:00:00

abstract：:The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best...

journal_title：Applied biochemistry and biotechnology

authors： Bernardes OL,Bevilaqua JV,Leal MC,Freire DM,Langone MA

更新日期：2007-04-01 00:00:00

abstract：:Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

journal_title：Applied biochemistry and biotechnology

authors： Kim M,Aita G,Day DF

更新日期：2010-05-01 00:00:00

abstract：:A new method without any special devices for direct transformation of linear gene cassettes was developed. Its feasibility was verified through 5'-fluorescent dye (fluorescein isothiocyanate, FITC)-labeled fluorescent tracing and transient expression of a gus reporter gene. Minimal linear gene cassettes, containing ne...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YQ,Yang J,Xu FP,An LJ,Liu JF,Chen ZW

更新日期：2009-12-01 00:00:00

abstract：:The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Jones F,Bailey R,Wilson S,Hiestand J

更新日期：2007-04-01 00:00:00

abstract：:The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs proliferation has yet been reported. In the present study, the feas...

journal_title：Applied biochemistry and biotechnology

authors： Yu Y,Li K,Bao C,Liu T,Jin Y,Ren H,Yun W

更新日期：2009-10-01 00:00:00

abstract：:Aspergillus niger CTBU isolated from local decayed bamboo shoot residue was employed to solid-state fermentation (SSF) of bamboo shoot residue to elevate the content of phytosterols. Strain acclimatization was carried out under the fermentation condition using bamboo shoot as substrate for fermentation performance imp...

journal_title：Applied biochemistry and biotechnology

authors： Zheng XX,Chen RS,Shen Y,Yin ZY

更新日期：2014-04-01 00:00:00

abstract：:The recombinant Escherichia coli gamma-glutamyltranspeptidase (EcGGT) was immobilized in Ca-alginate-kappa-carrageenan beads. Effects of alginate concentration, amount of loading enzyme, and bead size on the entrapped activity were investigated. Optimum alginate concentration for EcGGT immobilization was found to be 2...

journal_title：Applied biochemistry and biotechnology

authors： Hung CP,Lo HF,Hsu WH,Chen SC,Lin LL

更新日期：2008-08-01 00:00:00