Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System.

Abstract:

:To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.25 and 100 μg/mL for both methods. The clenbuterol limit of detection for the target site compensation method and solution competition method are estimated to be 6.7 and 4.5 μg/mL, respectively. The proposed methods were successfully applied to the detection of clenbuterol molecules and were found to have high specificity and high-throughput and were label free and operationally convenient.

authors

Wu Y,Yao M,Fang X,Yang Y,Cheng X

doi

10.1007/s12010-015-1817-6

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

1327-37

issue

6

eissn

0273-2289

issn

1559-0291

pii

10.1007/s12010-015-1817-6

journal_volume

177

pub_type

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