ACE-Inhibitory and Antioxidant Activities of Peptide Fragments Obtained from Tomato Processing By-Products Fermented Using Bacillus subtilis: Effect of Amino Acid Composition and Peptides Molecular Mass Distribution.

abstract：:The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

journal_title：Applied biochemistry and biotechnology

authors： Sousa CA,Hanselaer S,Soares EV

更新日期：2015-01-01 00:00:00

abstract：:A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptase...

journal_title：Applied biochemistry and biotechnology

authors： Omidinia E,Shadjou N,Hasanzadeh M

更新日期：2014-02-01 00:00:00

abstract：:Fuel ethanol can be produced from softwood through hydrolysis in an enzymatic process. Prior to enzymatic hydrolysis of the softwood, pretreatment is necessary. In this study, two-step steam pretreatment employing dilute H2SO4 impregnation in the first step and SO2 impregnation in the second step, to improve the overa...

journal_title：Applied biochemistry and biotechnology

authors： Söderström J,Pilcher L,Galbe M,Zacchi G

更新日期：2003-04-01 00:00:00

abstract：:Ovarian cancer lacks clear syndromes at an early stage and could result in serious problem in woman's health status. The current diagnostic approach relies on physical examination, ultrasound examination, and blood test for CA125. These approaches could not diagnose early stage ovarian cancer with high sensitivity and...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Y,Guo B,Bi R

更新日期：2012-10-01 00:00:00

abstract：:An anaerobic sequencing batch biofilm reactor (AnSBBR-total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 °C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m(-3) day(-1)), which were varied...

journal_title：Applied biochemistry and biotechnology

authors： Manssouri M,Rodrigues JA,Ratusznei SM,Zaiat M

更新日期：2013-12-01 00:00:00

abstract：:Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

journal_title：Applied biochemistry and biotechnology

authors： Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

更新日期：2012-07-01 00:00:00

abstract：:Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...

journal_title：Applied biochemistry and biotechnology

authors： Thierfelder S,Ostermann K,Göbel A,Rödel G

更新日期：2011-04-01 00:00:00

abstract：:The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of ...

journal_title：Applied biochemistry and biotechnology

authors： Abi A,Wang A,Jördening HJ

更新日期：2018-12-01 00:00:00

abstract：:Gluconacetobacter spp. synthesize a pure form of hydrophilic cellulose that has several industrial specialty applications. Literature reports have concentrated on intensive investigation of static and agitated culture in liquid media containing high nutrient concentrations optimized for maximal cellulose production ra...

journal_title：Applied biochemistry and biotechnology

authors： Evans BR,O'Neill HM

更新日期：2005-04-01 00:00:00

abstract：:Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to for...

journal_title：Applied biochemistry and biotechnology

authors： Bhuvana M,Dharuman V

更新日期：2014-10-01 00:00:00

abstract：:To facilitate structural studies of receptor-interacting protein 3 (RIP3), we developed a large-scale expression system of a glutathione-S-transferase (GST) fused with an 82 amino acid RIP3 protein in Escherichia coli. RIP3 truncation was subcloned into the pGEX-4T-1 vector and overexpressed in BL21(DE3)RIL cells. The...

journal_title：Applied biochemistry and biotechnology

authors： Jeong MS,Park JS,Jang SB

更新日期：2007-05-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:The mutant strain designated as ART18, obtained from the wild-type strain Clostridium acetobutylicum PW12 treated by atmospheric and room temperature plasma, showed higher solvent tolerance and butanol production than that of the wild-type strain. The production of butanol was 11.3 ± 0.5 g/L, 31 % higher than that of ...

journal_title：Applied biochemistry and biotechnology

authors： Li HG,Luo W,Wang Q,Yu XB

更新日期：2014-04-01 00:00:00

abstract：:The effect of four synthetic media containing glucose (initial concentration 30 g l(-1)) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also ...

journal_title：Applied biochemistry and biotechnology

authors： Diamantopoulou P,Papanikolaou S,Kapoti M,Komaitis M,Aggelis G,Philippoussis A

更新日期：2012-06-01 00:00:00

abstract：:Expression of exogenous DNA in vitro is significantly affected by the particular transfection method utilized. In this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart myoblast cell line, H9c2(2...

journal_title：Applied biochemistry and biotechnology

authors： Liu YC,Lin WY,Jhang YR,Huang SH,Wu CP,Wu HT

更新日期：2011-08-01 00:00:00

abstract：:Protein-protein interactions (PPIs) play pivotal roles in most of the biological processes. PPI dysfunctions are therefore associated with disease situations. Mutations often lead to PPI dysfunctions, but there are certain other types of mutations which do not cause any appreciable abnormalities. This second type of m...

journal_title：Applied biochemistry and biotechnology

authors： Ali A,Biswas R,Bhattacharjee S,Nath P,Pan S,Bagchi A

更新日期：2016-09-01 00:00:00

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:Polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. In this study, specific bacterial strains produced specific copolymers from food waste. Copolymers of HB and HV (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]...

journal_title：Applied biochemistry and biotechnology

authors： Wong PA,Chua H,Lo W,Lawford HG,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...

journal_title：Applied biochemistry and biotechnology

authors： Liao Y,Li CM,Chen H,Wu Q,Shan Z,Han XY

更新日期：2013-10-01 00:00:00

abstract：:An extracellular low molecular weight xylanase (Xyn628) from Streptomyces sp. CS628 was isolated from Korean soil sample, produced in wheat bran medium, purified, and biochemically characterized. Xyn628 was purified 4.8-fold with a 33.78 % yield using Sepharose CL-6B column chromatography. The purified xylanase was ~1...

journal_title：Applied biochemistry and biotechnology

authors： Rahman MA,Choi YH,Pradeep GC,Choi YS,Choi EJ,Cho SS,Yoo JC

更新日期：2014-07-01 00:00:00

abstract：:Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

journal_title：Applied biochemistry and biotechnology

authors： Pittner F

更新日期：1982-05-01 00:00:00

abstract：:Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

journal_title：Applied biochemistry and biotechnology

authors： Ramya LN,Doble M,Rekha VP,Pulicherla KK

更新日期：2012-08-01 00:00:00

abstract：:Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both enzymatic and acidic hydrolysis of xylosidic bonds. The alpha-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosacchari...

journal_title：Applied biochemistry and biotechnology

authors： Lee CC,Wagschal K,Kibblewhite-Accinelli RE,Orts WJ,Robertson GH,Wong DW

更新日期：2009-05-01 00:00:00

abstract：:The nuclear receptor Rev-erbβ is a potent transcriptional factor whose functional study has been limited by the lack of suitable antibodies against it. To better understand Rev-erbβ's biological roles, we generated five hybridoma cell lines secreting antibodies against human Rev-erbβ in mice immunized with the purifie...

journal_title：Applied biochemistry and biotechnology

authors： Chen F,Li Y,Zhao J,Mao Q,Xia H

更新日期：2017-07-01 00:00:00

abstract：:Organic wastes are increasingly collected source separated, thus requiring additional treatment or recovery capacities for municipal biowastes, organic industrial wastes, as well as agroindustrial byproducts. In this study, we demonstrate that anaerobic digestion is preferentially suited for highwater- containing liqu...

journal_title：Applied biochemistry and biotechnology

authors： Braun R,Brachtl E,Grasmug M

更新日期：2003-04-01 00:00:00

abstract：:The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescenc...

journal_title：Applied biochemistry and biotechnology

authors： Xu J,Zhu W,Guo Y,Jiang C,Hussain N,Meng L,Lu S

更新日期：2017-02-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:Currently, fumaric acid is produced by catalytic isomerization of maleic acid in aqueous solutions at low pH. Being petroleum based, requiring catalyst, and producing vast amounts of by-products and wastewater, the production of fumaric acid from renewable resources by a "green" process is increasingly attractive. In ...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Song J,Tan T,Liu L

更新日期：2015-03-01 00:00:00

abstract：:A mesophilic bacterial culture producing a novel thermostable alkaline lipase was isolated from oil rich soil sample and identified as Bacillus subtilis EH 37. The lipase was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography with 17.8-fold purification and 41.9 U/ml specif...

journal_title：Applied biochemistry and biotechnology

authors： Ahmed EH,Raghavendra T,Madamwar D

更新日期：2010-04-01 00:00:00

abstract：:Gold nanomaterials (Au NMs) have attracted increasing attention in biomedicine due to their facile preparation, multifunctional modifications, unique optical and electrical properties, and good biocompatibility. The physicochemical properties of Au NMs at nanoscale, like size, shape, surface chemistry, and near field ...

journal_title：Applied biochemistry and biotechnology

authors： Jiang XM,Wang LM,Wang J,Chen CY

更新日期：2012-03-01 00:00:00