The Influence of Reaction Conditions on DNA Multimerization During Isothermal Amplification with Bst exo- DNA Polymerase.

abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

更新日期：2016-07-01 00:00:00

abstract：:Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

journal_title：Applied biochemistry and biotechnology

authors： Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

更新日期：2011-06-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:Performance of microbial carbon capture cells (MCCs), having a low-cost clayware separator, was evaluated in terms of wastewater treatment and electricity generation using algae Chlorella pyrenoidosa in MCC-1 and Anabaena ambigua in MCC-2 and without algae in a cathodic chamber of MCC-3. Higher power production was ac...

journal_title：Applied biochemistry and biotechnology

authors： Jadhav DA,Jain SC,Ghangrekar MM

更新日期：2017-11-01 00:00:00

abstract：:The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene...

journal_title：Applied biochemistry and biotechnology

authors： Sturesson S,Tjerneld F,Johansson G

更新日期：1990-12-01 00:00:00

abstract：:Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

journal_title：Applied biochemistry and biotechnology

authors： Hassani L,Nourozi R

更新日期：2014-04-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:Increased demand for ethanol as a fuel additive has resulted in dramatic growth in ethanol production. Ethanol is produced from corn by either wet milling or dry-grind processing. In wet milling, the corn kernel is fractionated into different components, resulting in several coproducts. Wet-milling plants are capital ...

journal_title：Applied biochemistry and biotechnology

authors： Rausch KD,Belyea RL

更新日期：2006-01-01 00:00:00

abstract：:The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of ...

journal_title：Applied biochemistry and biotechnology

authors： Abi A,Wang A,Jördening HJ

更新日期：2018-12-01 00:00:00

abstract：:Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This ...

journal_title：Applied biochemistry and biotechnology

authors： Jonas R,Silveira MM

更新日期：2004-07-01 00:00:00

abstract：:EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully iso...

journal_title：Applied biochemistry and biotechnology

authors： Fang HY,Chen SC,Chen SL

更新日期：2003-11-01 00:00:00

abstract：:The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fec...

journal_title：Applied biochemistry and biotechnology

authors： Jena PK,Singh S,Prajapati B,Nareshkumar G,Mehta T,Seshadri S

更新日期：2014-04-01 00:00:00

abstract：:The capability to grow microalgae in nonsterilized wastewater is essential for an application of this technology in an actual industrial process. Batch experiments were carried out with the species in nonsterilized urban wastewater from local treatment plants to measure both the algal growth and the nutrient consumpti...

journal_title：Applied biochemistry and biotechnology

authors： Ramos Tercero EA,Sforza E,Morandini M,Bertucco A

更新日期：2014-02-01 00:00:00

abstract：:The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-...

journal_title：Applied biochemistry and biotechnology

authors： Han J,Xue Y,Li M,Li Y,Liu J,Gan L,Long M

更新日期：2020-08-01 00:00:00

abstract：:HbA1c test has been widely used to evaluate glycemic control in patients with diabetes. However, there are controversial results regarding the value of HbA1c in the diagnosis of diabetes mellitus (DM). The present study investigates the diagnostic effectiveness of HbA1c in a large patient group. The oral glucose toler...

journal_title：Applied biochemistry and biotechnology

authors： Serdar MA,Serteser M,Ucal Y,Karpuzoglu HF,Aksungar FB,Coskun A,Kilercik M,Ünsal İ,Özpınar A

更新日期：2020-01-01 00:00:00

abstract：:Bacillus amyloliquefaciens LL3 is a glutamate-independent poly-γ-glutamic acid (γ-PGA) producing strain which consists of a circular chromosome (3,995,227 bp) and an endogenous plasmid pMC1 (6,758 bp). The study of the function of native plasmid and the genome-size reduction of the B. amyloliquefaciens LL3 strain requ...

journal_title：Applied biochemistry and biotechnology

authors： Feng J,Gu Y,Wang J,Song C,Yang C,Xie H,Zhang W,Wang S

更新日期：2013-09-01 00:00:00

abstract：:In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

journal_title：Applied biochemistry and biotechnology

authors： Seidi A,Mie M,Kobatake E

更新日期：2009-08-01 00:00:00

abstract：:To investigate the effect of pH and temperature on the cell growth and bacteriocin production of Pediococcus acidilactici PA003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of P. acidilactici PA003 was simulated in vitro during laboratory fermentations by making use of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Zhang Y,Liu SN,Han Y,Zhou ZJ

更新日期：2012-03-01 00:00:00

abstract：:Electrokinetic-microbial remediation (EMR) has emerged as a promising option for the removal of polycyclic aromatic hydrocarbons (PAHs) from contaminated soils. The aim of this study was to enhance degradation of phenanthrene (Phe)-contaminated soils using EMR combined with biosurfactants. The electrokinetic (EK) reme...

journal_title：Applied biochemistry and biotechnology

authors： Lin W,Guo C,Zhang H,Liang X,Wei Y,Lu G,Dang Z

更新日期：2016-04-01 00:00:00

abstract：:Different initial cell concentrations of a recombinant flocculent Saccharomyces cerevisiae MA-R4 were evaluated for their effects on xylose fermentation and glucose-xylose cofermentation. A high initial cell concentration greatly increased both the substrate utilization and ethanol production rates. During xylose ferm...

journal_title：Applied biochemistry and biotechnology

authors： Matsushika A,Sawayama S

更新日期：2010-11-01 00:00:00

abstract：:Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Feather-degrading bacteria were isolated from a Brazilian poultry industrial waste. Among these isolates, a strain identified as kr2 was th...

journal_title：Applied biochemistry and biotechnology

authors： Sangali S,Brandelli A

更新日期：2000-04-01 00:00:00

abstract：:RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not ...

journal_title：Applied biochemistry and biotechnology

authors： Nübel C,Appel B,Hospach I,Mai M,Krasteva N,Nelles G,Petruschka L,Müller S

更新日期：2016-06-01 00:00:00

abstract：:The unprecedented coronavirus SARS-CoV-2 outbreak at Wuhan, China, caused acute respiratory infection to humans. There is no precise vaccine/therapeutic agents available to combat the COVID-19 disease. Some repurposed drugs are saving the life of diseased, but the complete cure is relatively less. Several drug targets...

journal_title：Applied biochemistry and biotechnology

authors： Ramakrishnan J,Kandasamy S,Iruthayaraj A,Magudeeswaran S,Chinnasamy K,Poomani K

更新日期：2021-01-29 00:00:00

abstract：:The Pichia stipitis xylose reductase gene (XYL1) was inserted into an autonomous plasmid that P. stipitis maintains in multicopy. The plasmid pXOR with the XYL1 insert or a control plasmid pJM6 without XYL1 was introduced into P. stipitis. When grown on xylose under aerobic conditions, the strain with pXOR had up to 1...

journal_title：Applied biochemistry and biotechnology

authors： Dahn KM,Davis BP,Pittman PE,Kenealy WR,Jeffries TW

更新日期：1996-04-01 00:00:00

abstract：:Sequencing batch reactors (SBRs) including aerobic SBRs and anaerobic SBRs (ASBRs) are partially emptied batch reactors that are widely used as bioprocesses in pollution control. We present dilution and biochemical materials balance modeling equations and simulation results for the partially emptied batch reactors, es...

journal_title：Applied biochemistry and biotechnology

authors： Ndon UJ,Zhao JC,Siguan ML

更新日期：2007-01-01 00:00:00

abstract：:cDNA coding a prolyl aminopeptidase (PAP) was cloned from Aspergillus oryzae and over expressed in Bacillus subtilis with a 6×His tag in N-terminus. The recombinant prolyl aminopeptidase was secreted to extracellular by adding 2 mM CaCl2 and 5% D-sorbitol in TB medium; the enzyme activity in fermented supernatant incr...

journal_title：Applied biochemistry and biotechnology

authors： Wang KD,Wang KH,Zhou ND,Tian YP

更新日期：2017-04-01 00:00:00

abstract：:Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

journal_title：Applied biochemistry and biotechnology

authors： Bollók M,Réczey K,Zacchi G

更新日期：2000-04-01 00:00:00

abstract：:Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

journal_title：Applied biochemistry and biotechnology

authors： Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

更新日期：2016-08-01 00:00:00

abstract：:Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

journal_title：Applied biochemistry and biotechnology

authors： Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

更新日期：2014-08-01 00:00:00

abstract：:The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to p...

journal_title：Applied biochemistry and biotechnology

authors： Domingues VS,Monteiro AS,Ferreira GF,Santos VL

更新日期：2017-05-01 00:00:00