Efficient Secretory Overexpression of Endoinulinase in Escherichia coli and the Production of Inulooligosaccharides.

abstract：:An anaerobic sequencing batch biofilm reactor (AnSBBR-total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 °C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m(-3) day(-1)), which were varied...

journal_title：Applied biochemistry and biotechnology

authors： Manssouri M,Rodrigues JA,Ratusznei SM,Zaiat M

更新日期：2013-12-01 00:00:00

abstract：:Studies were carried out in a packed-bed column fermentor using coffee husk as substrate in order to verify a relationship between caffeine degradation and the respiration of Aspergillus sp. LPBx. Fermentation conditions were optimized by using factorial design experiments. The kinetic study showed that the caffeine d...

journal_title：Applied biochemistry and biotechnology

authors： Brand D,Pandey A,Rodriguez-Leon JA,Roussos S,Brand I,Soccol CR

更新日期：2002-07-01 00:00:00

abstract：:Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

journal_title：Applied biochemistry and biotechnology

authors： Yan J,Gui X,Wang G,Yan Y

更新日期：2012-02-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs which play significant role in RNA interference. The present work deals with mining of the conserved miRNA and their target genes from the contigs, ESTs, and BAC end sequences of commercially important catfish, Clarias batrachus, from India. A total of 138, 1 and 1 conserved pre-miR...

journal_title：Applied biochemistry and biotechnology

authors： Agarwal S,Nagpure NS,Srivastava P,Kumar R,Pandey M,Srivastava S,Jena JK,Das P,Kushwaha B

更新日期：2017-07-01 00:00:00

abstract：:More than two decades ago, a group of research engineers and applied scientists with interest in energy applications of bioprocessing initiated an annual symposium series ultimately entitled "Biotechnology for Fuels and Chemicals." The Department of Energy, several of the national laboratories, and various industrial ...

journal_title：Applied biochemistry and biotechnology

authors： Scott CD

更新日期：2004-04-01 00:00:00

abstract：:One-stage autoheated thermophilic aerobic digestion (ATAD) can stabilize sludge to meet class A standard. In this study, batch experiments were conducted to investigate the characteristics of oxidation-reduction potential (ORP), volatile fatty acids (VFAs), soluble chemical oxygen demand (SCOD), and nutrients at diffe...

journal_title：Applied biochemistry and biotechnology

authors： Cheng J,Kong F,Zhu J,Wu X

更新日期：2015-01-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00

abstract：:For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...

journal_title：Applied biochemistry and biotechnology

authors： Zhao L,Jiang Y,Fang H,Zhang H,Cheng S,Rajoka MSR,Wu Y

更新日期：2019-10-01 00:00:00

abstract：:Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [poly(HEMA-GMA)] cryogel was synthesized by cryopolymerization technique at frozen temperature. Iminodiacetic acid (IDA) was then attached covalently to the cryogel as a chelating agent. Then, poly(HEMA-GMA)-IDA cryogel was chelated with Ni(II) ions and this n...

journal_title：Applied biochemistry and biotechnology

authors： Uygun M,Akduman B,Akgöl S,Denizli A

更新日期：2013-08-01 00:00:00

abstract：:The methods for increasing the production of ergothioneine (ERG) were investigated by using the mycelial culture of several mushroom species, primarily Ganoderma neo-japonicum. We first found that ERG was accumulated at the different levels in mycelia and fruiting bodies, respectively, depending on the mushroom specie...

journal_title：Applied biochemistry and biotechnology

authors： Lee WY,Park EJ,Ahn JK

更新日期：2009-07-01 00:00:00

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:Different initial cell concentrations of a recombinant flocculent Saccharomyces cerevisiae MA-R4 were evaluated for their effects on xylose fermentation and glucose-xylose cofermentation. A high initial cell concentration greatly increased both the substrate utilization and ethanol production rates. During xylose ferm...

journal_title：Applied biochemistry and biotechnology

authors： Matsushika A,Sawayama S

更新日期：2010-11-01 00:00:00

abstract：:Five autotrophic strains isolated from non-photosynthetic microbial communities (NPMCs), which were screened from oceans with high CO2 fixing capability, were identified as Ochrobactrum sp. WH-2, Stenotrophomonas sp. WH-11, Ochrobactrum sp. WH-13, Castellaniella sp. WH-14, and Sinomicrobium oceani WH-15. The CO2 fixat...

journal_title：Applied biochemistry and biotechnology

authors： Hu J,Wang L,Zhang S,Xi X,Le Y,Fu X,Tsang Y,Gao M

更新日期：2015-07-01 00:00:00

abstract：:Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

journal_title：Applied biochemistry and biotechnology

authors： Clark KJ,Harcum SW,Griffiths J,Bailey KM

更新日期：2005-06-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:An Aspergillus terreus, isolated from oil contaminated soil, could degrade a wide range of petroleum hydrocarbons including the immediate oxidation products of hydrocarbons, like alkanols and alkanals. Among all the linear chain carbon substrates, highest growth of 39.1 + or - 3.8 g l(-1) (wet weight) was observed whe...

journal_title：Applied biochemistry and biotechnology

authors： Kumar AK,Vatsyayan P,Goswami P

更新日期：2010-03-01 00:00:00

abstract：:Oseltamivir (Tamiflu) is the preferred anti-viral drug employed to fight the flu virus in infected individuals. The principal target for this drug is a virus surface glycoprotein, neuraminidase (NA), which facilitates the release of nascent virus and thus spreads infections. Until recently, only a low prevalence of ne...

journal_title：Applied biochemistry and biotechnology

authors： Karthick V,Shanthi V,Rajasekaran R,Ramanathan K

更新日期：2012-05-01 00:00:00

abstract：:The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synt...

journal_title：Applied biochemistry and biotechnology

authors： Rathod JP,Vira C,Lali AM,Prakash G

更新日期：2020-04-01 00:00:00

abstract：:In the present investigation, thermophilic fungus Rhizomucor pusillus was used to study biotransformation of antihelmintic drug albendazole to produce its active metabolite, albendazole sulfoxide and novel metabolites of commercial interest. A two-stage fermentation procedure was followed for biotransformation of albe...

journal_title：Applied biochemistry and biotechnology

authors： Prasad GS,Girisham S,Reddy SM

更新日期：2011-11-01 00:00:00

abstract：:In a recombinant antibody scFv format, the presence of an unpaired cysteine (Cys) is implicated in reduced soluble expression and inefficient presentation in phage display. Compared to other species, antibodies derived from rabbits are more likely to contain this unpaired Cys residue at position 80 (Cys80), when gener...

journal_title：Applied biochemistry and biotechnology

authors： Ayyar BV,Hearty S,O'Kennedy R

更新日期：2018-07-01 00:00:00

abstract：:Increased demand for ethanol as a fuel additive has resulted in dramatic growth in ethanol production. Ethanol is produced from corn by either wet milling or dry-grind processing. In wet milling, the corn kernel is fractionated into different components, resulting in several coproducts. Wet-milling plants are capital ...

journal_title：Applied biochemistry and biotechnology

authors： Rausch KD,Belyea RL

更新日期：2006-01-01 00:00:00

abstract：:We expressed a putative beta-galactosidase from Sulfolobus acidocaldarius in Escherichia coli and purified the recombinant enzyme using heat treatment and Hi-Trap ion-exchange chromatography. The resultant protein gave a single 57-kDa band by SDS-PAGE and had a specific activity of 58 U/mg. The native enzyme existed a...

journal_title：Applied biochemistry and biotechnology

authors： Park AR,Kim HJ,Lee JK,Oh DK

更新日期：2010-04-01 00:00:00

abstract：:Pseudomonas are ubiquitous bacteria that can live under a wide range of environmental conditions; some species are successfully used in the fermentation industry. Pseudomonas plecoglossicida JUIM01 strain is an important industrial 2-ketogluconate (2KGA) producer. In Pseudomonas, kguT gene encodes a putative 2KGA tran...

journal_title：Applied biochemistry and biotechnology

authors： Sun W,Wang Q,Luan F,Man Z,Cui F,Qi X

更新日期：2019-03-01 00:00:00

abstract：:Two types of (CNBr-activated) Agarose-staphylococcal endonuclease derivatives have been prepared, one with the enzyme uniformly distributed in the support, and the other with the enzyme preferentially bound in the most external part of the support particles; the latter were obtained using agarose of very small pores a...

journal_title：Applied biochemistry and biotechnology

authors： Guisan JM,Serrano J,Melo FV,Ballesteros A

更新日期：1987-02-01 00:00:00

abstract：:The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

journal_title：Applied biochemistry and biotechnology

authors： Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

更新日期：2013-01-01 00:00:00

abstract：:Methanol, acetone and diethyl ether extracts of Alpinia galanga have been evaluated against pathogens viz. Bacillus subtilis MTCC 2391, Enterobacter aerogene, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli MTCC 1563, Klebsiella pneumoniae, Pseudomonas aeruginosa MTCC 6642, Salmonella typhimurium, Staphy...

journal_title：Applied biochemistry and biotechnology

authors： Rao K,Ch B,Narasu LM,Giri A

更新日期：2010-10-01 00:00:00

abstract：:During the aging of yeast culture, Kluyveromyces marxianus undergoes a number of changes in physiology and these changes play a significant role during fermentation. Aged stationary phase cells were found to contain more reactive oxygen species. Additionally, the level of oxidant is counteracted by the antioxidant def...

journal_title：Applied biochemistry and biotechnology

authors： Saini P,Beniwal A,Vij S

更新日期：2017-09-01 00:00:00

abstract：:Trehalose synthase (TreS) from Meiothermus ruber was co-aggregated with polyethyleneimine (PEI) and precipitated with polyethylene glycol (PEG), followed by cross-linking with glutaraldehyde to obtain TreS-polyethyleneimine cross-linked enzyme aggregates (termed as CLEAs-PEI-PEG). The TreS solution at 0.5 mg mL(-1) pr...

journal_title：Applied biochemistry and biotechnology

authors： Zheng J,Chen Y,Yang L,Li M,Zhang J

更新日期：2014-11-01 00:00:00

abstract：:A new, simple, and original method is described for specific measurement of polyunsaturated fatty acid content in olive oil. This analytical system uses coupled enzymes, lipase and lipoxygenase. The system consists of lipase-catalyzed hydrolysis of triacylglycerol and subsequent lipoxygenation of liberated polyunsatur...

journal_title：Applied biochemistry and biotechnology

authors： Ben Rejeb I,Monser L,Gargouri M

更新日期：2010-11-01 00:00:00

abstract：:Fed-batch method has shown a great promise in debottlenecking the high-solid enzymatic hydrolysis for the commercialization of cellulosic biosugar conversion for biofuel/biochemical production. To further improve enzymatic hydrolysis efficiency at high solid loading, fed-batch methods of green liquor-pretreated hardwo...

journal_title：Applied biochemistry and biotechnology

authors： He L,Han Q,Jameel H,Chang HM,Phillips R,Wang Z

更新日期：2018-04-01 00:00:00