Mixed enzymic reaction--internal diffusion kinetics of nonuniformly distributed immobilized enzymes. The system agarose-micrococcal endonuclease.

Abstract:

:Two types of (CNBr-activated) Agarose-staphylococcal endonuclease derivatives have been prepared, one with the enzyme uniformly distributed in the support, and the other with the enzyme preferentially bound in the most external part of the support particles; the latter were obtained using agarose of very small pores and a high degree of activation. Quantitative enzyme distribution has been determined by scanning fluorescence microscopy. With these insoluble enzyme derivatives, a kinetic study for the hydrolysis of a mononucleotide has been carried out. A simple theoretical model for nonuniformly distributed insoluble enzyme derivatives, which considers only the case of mixed enzymic reaction-internal diffusion kinetics, is proposed. The experimental data agree very well with the predictions of the model.

authors

Guisan JM,Serrano J,Melo FV,Ballesteros A

doi

10.1007/BF02798498

subject

Has Abstract

pub_date

1987-02-01 00:00:00

pages

49-72

issue

1

eissn

0273-2289

issn

1559-0291

journal_volume

14

pub_type

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