Abstract:
:The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synthase (psy) and lycopene cyclization (lcyb) activities. Heterologous expression of this bifunctional PBS gene led to 38% enhancement in β-carotene along with 60% increase in the lutein yields under low light conditions of 75 μmol photons m-2 s-1. Short Duration-High Light induction strategy led to overall 72% and 83% increase in β-carotene and lutein yield reaching up to 22.8 mg g-1 and 8.9 mg g-1, respectively. This is the first report of expression of heterologous bifunctional PBS gene resulting in simultaneous enhancement in β-carotene and lutein content in phototrophic engineered cells. Graphical Abstract.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Rathod JP,Vira C,Lali AM,Prakash Gdoi
10.1007/s12010-019-03194-9subject
Has Abstractpub_date
2020-04-01 00:00:00pages
1457-1469issue
4eissn
0273-2289issn
1559-0291pii
10.1007/s12010-019-03194-9journal_volume
190pub_type
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