Lactobacillus plantarum amylase acting on crude starch granules. Native isoforms and activity changes after limited proteolysis.

abstract：:Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

journal_title：Applied biochemistry and biotechnology

authors： Hassani L,Nourozi R

更新日期：2014-04-01 00:00:00

abstract：:The present study was undertaken to evaluate the effect of initial temperature treatment on phytochemical and antioxidant potential of commercially important plant Azadirachta indica A. Juss. The leaves were differentially treated after harvest at temperatures 4, -20, and 110 °C and at room temperature, separately. It...

journal_title：Applied biochemistry and biotechnology

authors： Vats S

更新日期：2016-02-01 00:00:00

abstract：:A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. ...

journal_title：Applied biochemistry and biotechnology

authors： Puri N,Niazi A,Srivastava AK,Rajesh

更新日期：2014-10-01 00:00:00

abstract：:The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

journal_title：Applied biochemistry and biotechnology

authors： Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

更新日期：1999-04-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...

journal_title：Applied biochemistry and biotechnology

authors： Sharma SC,Anand MS

更新日期：2006-04-01 00:00:00

abstract：:The use of stalks instead of tubers as a source of carbohydrates for ethanol production has been investigated. The inulin present in the stalks of Jerusalem artichoke was extracted with water and the effect of solid-liquid ratio, temperature, and acid addition was studied and optimized in order to attain a high-fructo...

journal_title：Applied biochemistry and biotechnology

authors： Negro MJ,Ballesteros I,Manzanares P,Oliva JM,Sáez F,Ballesteros M

更新日期：2006-04-01 00:00:00

abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...

journal_title：Applied biochemistry and biotechnology

authors： Liao Y,Li CM,Chen H,Wu Q,Shan Z,Han XY

更新日期：2013-10-01 00:00:00

abstract：:Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated...

journal_title：Applied biochemistry and biotechnology

authors： Hu C,Choy SY,Giannis A

更新日期：2018-05-01 00:00:00

abstract：:Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditio...

journal_title：Applied biochemistry and biotechnology

authors： Deliormanlı AM,Atmaca H

更新日期：2018-12-01 00:00:00

abstract：:The effect of different concentrations of zinc on the bioaccumulation of zinc and biomass yield in both mycelium and fruiting body of Pleurotus florida cultivated in liquid medium was studied. The results showed that the optimum yield of mycelia (11.33 ± 0.44 g/L) and fruiting bodies (7.70 ± 0.19 g/L) dry biomass was ...

journal_title：Applied biochemistry and biotechnology

authors： Poursaeid N,Azadbakht A,Balali GR

更新日期：2015-04-01 00:00:00

abstract：:An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish peroxidase, 4-amino-phenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis, with free and immobilized enzymes. The system with free enzymes pe...

journal_title：Applied biochemistry and biotechnology

authors： Alhadeff EM,Salgado AM,Pereira N Jr,Valdman B

更新日期：2004-04-01 00:00:00

abstract：:Nanotechnology is an emerging technology which is an amalgamation of different aspects of science and technology that includes disciplines such as electrical engineering, mechanical engineering, biology, physics, chemistry, and material science. It has potential in the fields of information and communication technolog...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty M,Jain S,Rani V

更新日期：2011-11-01 00:00:00

abstract：:In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high ac...

journal_title：Applied biochemistry and biotechnology

authors： Zhou HY,Pan HY,Rao LQ,Wu YY

更新日期：2011-01-01 00:00:00

abstract：:L-asparaginase production was optimized using isolated Bacillus circulans (MTCC 8574) under solid-state fermentation (SSF) using locally available agricultural waste materials. Among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme p...

journal_title：Applied biochemistry and biotechnology

authors： Hymavathi M,Sathish T,Subba Rao Ch,Prakasham RS

更新日期：2009-10-01 00:00:00

abstract：:Xanthomonas campestris w.t. was used for production of xanthan gum in fermentations with chestnut flour for the first time. Fermentations were carried out with either chestnut flour or its soluble sugars (33.5%) and starch (53.6%), respectively, at 28 degrees C and 200 rpm at initial pH 7.0 in flasks. The effect of ag...

journal_title：Applied biochemistry and biotechnology

authors： Liakopoulou-Kyriakides M,Psomas SK,Kyriakidis DA

更新日期：1999-12-01 00:00:00

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of usi...

journal_title：Applied biochemistry and biotechnology

authors： Niyonzima FN,More SS

更新日期：2014-10-01 00:00:00

abstract：:Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...

journal_title：Applied biochemistry and biotechnology

authors： Kasra-Kermanshahi R,Tajer-Mohammad-Ghazvini P,Bahrami-Bavani M

更新日期：2020-11-17 00:00:00

abstract：:Strain M(438), deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-pro...

journal_title：Applied biochemistry and biotechnology

authors： Ge HJ,Du SK,Lin DH,Zhang JN,Xiang JL,Li ZX

更新日期：2011-12-01 00:00:00

abstract：:Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

journal_title：Applied biochemistry and biotechnology

authors： Rodrigues DC,Silva SS,Prata AM,Felipe MG

更新日期：1998-04-01 00:00:00

abstract：:Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...

journal_title：Applied biochemistry and biotechnology

authors： Shah A,Priyadarshini M,Khan MS,Aatif M,Amin F,Bano B

更新日期：2013-10-01 00:00:00

abstract：:The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-...

journal_title：Applied biochemistry and biotechnology

authors： Han J,Xue Y,Li M,Li Y,Liu J,Gan L,Long M

更新日期：2020-08-01 00:00:00

abstract：:Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

journal_title：Applied biochemistry and biotechnology

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2005-03-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT, EC 2.6.1.1) was bound on CNBr-activated Sepharose and the effects of immobilization on the maximum velocity, biologically active pyridoxal-5'-phosphate (PLP), and transaminationable active centers were studied. By comparing these parameters of soluble and immobilized enzyme the facto...

journal_title：Applied biochemistry and biotechnology

authors： Kurkijärvi K,Korpela T

更新日期：1983-04-01 00:00:00

abstract：:The use of cheap, high-efficiency, and ecofriendly adsorbent has been studied as an alternative way for the removal of dyes from wastewater. This paper investigated the use of waste brewery's yeast for the removal of acid orange 7 from aqueous solution. The optimum removal of acid orange 7 was found to be 3.561 mg/g a...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Hu Y,Xie Z,Feng S,Li B,Mi X

更新日期：2011-04-01 00:00:00

abstract：:A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...

journal_title：Applied biochemistry and biotechnology

authors： Mohanraj S,Kodhaiyolii S,Rengasamy M,Pugalenthi V

更新日期：2014-05-01 00:00:00

abstract：:The cellulolytic fungus, Trichoderma has oval and mononucleate conidia. When these conidia are incubated in a liquid medium, they begin to swell and their shape becomes spherical followed by an increase in inner space. In such swollen conidia, it is possible to produce a larger autopolyploid nucleus using a mitotic ar...

journal_title：Applied biochemistry and biotechnology

authors： Toyama H,Yano M,Hotta T

更新日期：2004-04-01 00:00:00

abstract：:Aspergillus fumigatus N2 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest xylanase (91.9 U/mL) and CMCase (5.61 U/mL) activity was produced when 1% barley straw was used as the carbon source. The optimum pH and temperature for xylanase activity were 6.0 and 65 °...

journal_title：Applied biochemistry and biotechnology

authors： Lin C,Shen Z,Qin W

更新日期：2017-06-01 00:00:00

abstract：:Rice straw hemicellulosic hydrolysate was used as fermentation medium for ethanol production by Pichia stipitis NRRL Y-7124. Shaking bath experiments were initially performed aiming to establish the best initial xylose concentration to be used in this bioconversion process. In the sequence, assays were carried out und...

journal_title：Applied biochemistry and biotechnology

authors： Silva JP,Mussatto SI,Roberto IC

更新日期：2010-11-01 00:00:00