Synthesis and characterization of reduced graphene oxide supported gold nanoparticles-poly(pyrrole-co-pyrrolepropylic acid) nanocomposite-based electrochemical biosensor.

abstract：:A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...

journal_title：Applied biochemistry and biotechnology

authors： Caudan C,Filali A,Lefebvre D,Spérandio M,Girbal-Neuhauser E

更新日期：2012-04-01 00:00:00

abstract：:We have developed a substrate-induced growth response (SIGR) method for quantifying activity and population dynamics of microorganisms involved in bioremediation processes in soil and bioreactors. The biomass of organisms that can mineralize a given chemical can be estimated based on the concentration of that chemical...

journal_title：Applied biochemistry and biotechnology

authors： Schmidt SK,Colores GM,Hess TF,Radehaus PM

更新日期：1995-07-01 00:00:00

abstract：:NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...

journal_title：Applied biochemistry and biotechnology

authors： Wienhausen GK,Kricka LJ,Hinkley JE,Deluca M

更新日期：1982-11-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:We expressed a putative beta-galactosidase from Sulfolobus acidocaldarius in Escherichia coli and purified the recombinant enzyme using heat treatment and Hi-Trap ion-exchange chromatography. The resultant protein gave a single 57-kDa band by SDS-PAGE and had a specific activity of 58 U/mg. The native enzyme existed a...

journal_title：Applied biochemistry and biotechnology

authors： Park AR,Kim HJ,Lee JK,Oh DK

更新日期：2010-04-01 00:00:00

abstract：:The bacterium Enterobacter A47 has demonstrated the ability to synthesise distinct exopolysaccharides (EPS) as a function of the substrate used. The culture's performance was evaluated in experiments using either glucose or xylose, as single carbon sources, and compared with the substrate (glycerol) used in previous s...

journal_title：Applied biochemistry and biotechnology

authors： Freitas F,Alves VD,Gouveia AR,Pinheiro C,Torres CA,Grandfils C,Reis MA

更新日期：2014-01-01 00:00:00

abstract：:The abilities of lignin peroxidase (LIP) and manganese peroxidase (MNP) from Phanerochaete chrysosporium to degrade an insoluble hardwood lignin in vitro in aqueous media were tested. Neither LIP nor MNP appreciably changed the mass or lignin content, although both produced small amounts of unique solubilized lignin f...

journal_title：Applied biochemistry and biotechnology

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-04-01 00:00:00

abstract：:Equipment and procedures were developed to study the conversion of lignocellulosic biomass to carboxylic acids using high-solids countercurrent fermentations. Countercurrent fermentations of cattle manure yielded a rapid fermentation (maximum 2.98 g of total acid/[L x d]) with high acid concentrations (maximum of 32.5...

journal_title：Applied biochemistry and biotechnology

authors： Ross MK,Holtzapple MT

更新日期：2001-05-01 00:00:00

abstract：:The article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with Candida rugosa lipase within a chemical inert sol-gel supported by polycondensation w...

journal_title：Applied biochemistry and biotechnology

authors： Ozyilmaz E,Sayin S

更新日期：2013-08-01 00:00:00

abstract：:The separation and purification of biologically functional molecules (e.g., proteins, antibodies, peptides, hormones, low molecular weight biologicals) is of fundamental importance to biotechnology. Affinity separations have become a particularly attractive method for bioseparations due to their high degree of selecti...

journal_title：Applied biochemistry and biotechnology

authors： Dordick JS

更新日期：1991-01-01 00:00:00

abstract：:Commercial conversion of lignocellulosic biomass to fermentable sugars requires inexpensive bulk production of biologically active cellulase enzymes, which might be achieved through direct production of these enzymes within the biomass crops. Transgenic corn plants containing the catalytic domain of Acidothermus cellu...

journal_title：Applied biochemistry and biotechnology

authors： Ransom C,Balan V,Biswas G,Dale B,Crockett E,Sticklen M

更新日期：2007-04-01 00:00:00

abstract：:Cancer normally tends to result in the decrease of tissue elasticity; i.e., the cancerous region is more rigid than the normal surrounding areas. This would appear as differences in the distribution of internal birefringence that could be used to improve image contrast between the cancerous and normal tissue structure...

journal_title：Applied biochemistry and biotechnology

authors： Mathur N,Chaudhary NK,Bharti P

更新日期：2009-11-01 00:00:00

abstract：:Thermoacidophiles are microorganisms capable of optimum growth under a combination of high temperature and low pH. These microorganisms are a rich source of thermo- and acid- active/stable glycosyl hydrolases. Such enzymes could find use as novel biocatalysts in industrial processes, as operation at elevated temperatu...

journal_title：Applied biochemistry and biotechnology

authors： Murphy J,Ryan MP,Walsh G

更新日期：2020-07-01 00:00:00

abstract：:Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the mo...

journal_title：Applied biochemistry and biotechnology

authors： Jana A,Maity C,Halder SK,Mondal KC,Pati BR,Mohapatra PK

更新日期：2012-07-01 00:00:00

abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

journal_title：Applied biochemistry and biotechnology

authors： Tadolini B,Hakim G

更新日期：1981-09-01 00:00:00

abstract：:Reactive oxygen species (ROS) in plants, arising from various environmental stresses, impair the thiol-contained proteins that are susceptible to irregular oxidative formation of disulfide bonds, which might be alleviated by a relatively specific modifier called protein disulfide isomerase (PDI). From our previous dat...

journal_title：Applied biochemistry and biotechnology

authors： Wang H,Zou Z,Gong M

更新日期：2015-05-01 00:00:00

abstract：:In order to detect low levels of enzyme activity, specifically glucose oxidase, in biological samples, an immunoenzymatic assay was developed since currently available methods could not be used because of either their lack of sensitivity or the conditions prevailing in our samples: turbidity of the medium, presence of...

journal_title：Applied biochemistry and biotechnology

authors： de Bellefontaine C,Josse F,Domurado M,Domurado D

更新日期：1994-08-01 00:00:00

abstract：:Concentrated dilute acid hydrolysate was obtained from hot water extracts of Acer saccharum (sugar maple) and was fermented to ethanol by Pichia stipitis in a 1.3-L-benchtop bioreactor. The conditions under which the highest ethanol yield was achieved were when the air flow rate was set to 100 cm(3) and the agitation ...

journal_title：Applied biochemistry and biotechnology

authors： Shupe AM,Liu S

更新日期：2012-09-01 00:00:00

abstract：:The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fec...

journal_title：Applied biochemistry and biotechnology

authors： Jena PK,Singh S,Prajapati B,Nareshkumar G,Mehta T,Seshadri S

更新日期：2014-04-01 00:00:00

abstract：:To provide a mathematical basis for the design and operation of a continuous, packed-bed reactor for the interesterification of soybean oil, soybean oil that contains 22.7% oleoyl and 54.3% linoleoyl moieties as molar acyl moiety composition was interesterified in hexane with oleic acid, using an immobilized sn-1,3-sp...

journal_title：Applied biochemistry and biotechnology

authors： Akimoto M,Nagashima Y,Sato D

更新日期：1999-08-01 00:00:00

abstract：:Endophytes from medicinal plants represent a potential source of bioactive compounds. During the present investigation, fungal endophytes were isolated from turmeric (Curcuma longa), an important medicinal plant. A total of 207 endophytic fungal isolates were obtained from the rhizome of C. longa L. They were grouped ...

journal_title：Applied biochemistry and biotechnology

authors： Gupta S,Kaul S,Singh B,Vishwakarma RA,Dhar MK

更新日期：2016-11-01 00:00:00

abstract：:The present work evaluates the phenol degradative performance of microalgae Chlorella pyrenoidosa. High-performance liquid chromatography (HPLC) analysis showed that C. pyrenoidosa degrades phenol completely up to 200 mg/l. It could also metabolize phenol in refinery wastewater. Biokinetic parameters obtained are the ...

journal_title：Applied biochemistry and biotechnology

authors： Das B,Mandal TK,Patra S

更新日期：2015-07-01 00:00:00

abstract：:The objective of the present work was to evaluate the metabolic effects induced by the initial glucose concentration (IGC) on the cultivation of Streptococcus zooepidemicus for the production of hyaluronic acid (HA). These effects were monitored along non-controlled pH cultivations, carried out in 250-mL Erlenmeyer fl...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Santana MH

更新日期：2010-11-01 00:00:00

abstract：:The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...

journal_title：Applied biochemistry and biotechnology

authors： Yi X,Luo K,Yang Q,Li XM,Deng WG,Cheng HB,Wang ZL,Zeng GM

更新日期：2013-11-01 00:00:00

abstract：:When dilute-acid hydrolysates from spruce are fermented to produce ethanol, detoxification is required to make the hydrolysates fermentable at reasonable rates. Treatment with alkali, usually by overliming, is one of the most efficient approaches. Several nutrients, such as ammonium and phosphate, are added to the hyd...

journal_title：Applied biochemistry and biotechnology

authors： Alriksson B,Horváth IS,Sjöde A,Nilvebrant NO,Jönsson LJ

更新日期：2005-04-01 00:00:00

abstract：:The effect of methanol on trichloroethylene (TCE) degradation by mixed and pure methylotrophic cultures was examined in batch culture experiments. Methanol was found to relieve growth inhibition of Methylosinus trichosporium (OB3b) at high (14 mg/L) TCE concentrations. Degradation of TCE was determined by both radiola...

journal_title：Applied biochemistry and biotechnology

authors： Eng W,Palumbo AV,Sriharan S,Strandberg GW

更新日期：1991-04-01 00:00:00

abstract：:In this study, immobilization conditions and bioethanol production characteristics of immobilized Saccharomyces bayanus were investigated into sodium alginate-graft-poly(N-vinyl-2-pyrrolidone; NaAlg-g-PVP) matrix. The matrix that crosslinked with calcium clorid was used for immobilization of S. bayanus. Bioethanol pro...

journal_title：Applied biochemistry and biotechnology

authors： İnal M,Yiğitoğlu M

更新日期：2012-09-01 00:00:00

abstract：:Palm kernel press cake (PKC) is a residue from palm oil extraction presently only used as a low protein feed supplement. PKC contains 50% fermentable hexose sugars present in the form of glucan and mainly galactomannan. This makes PKC an interesting feedstock for processing into bioethanol or in other biorefinery proc...

journal_title：Applied biochemistry and biotechnology

authors： Jørgensen H,Sanadi AR,Felby C,Lange NE,Fischer M,Ernst S

更新日期：2010-05-01 00:00:00

abstract：:The Pichia stipitis xylose reductase gene (XYL1) was inserted into an autonomous plasmid that P. stipitis maintains in multicopy. The plasmid pXOR with the XYL1 insert or a control plasmid pJM6 without XYL1 was introduced into P. stipitis. When grown on xylose under aerobic conditions, the strain with pXOR had up to 1...

journal_title：Applied biochemistry and biotechnology

authors： Dahn KM,Davis BP,Pittman PE,Kenealy WR,Jeffries TW

更新日期：1996-04-01 00:00:00

abstract：:Bioethanol is considered a potential liquid fuel that can be produced from biomass by fermentation and distillation. Although most of the water is removed by distillation, the purity of ethanol is limited to 95-96 % due to the formation of a low-boiling point, water-ethanol azeotrope. To improve the use of ethanol as ...

journal_title：Applied biochemistry and biotechnology

authors： Tang B,Bi W,Row KH

更新日期：2013-02-01 00:00:00