Ammonium hydroxide detoxification of spruce acid hydrolysates.

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:Microalgae are photosynthetic microorganisms that produce several bioactive molecules that have received considerable attention in scientific and industrial communities. Today, many plant biostimulants including seaweed extracts and polysaccharides are used in agriculture. However, microalgae have not been largely exp...

journal_title：Applied biochemistry and biotechnology

authors： Farid R,Mutale-Joan C,Redouane B,Mernissi Najib EL,Abderahime A,Laila S,Arroussi Hicham EL

更新日期：2019-05-01 00:00:00

abstract：:The fermentative production of lactic acid from cheese whey and corn steep liquor (CSL) as cheap raw materials was investigated by using Lactobacillus sp. RKY2 in order to develop a cost-effective fermentation medium. Lactic acid yields based on consumed lactose were obtained at more than 0.98 g/g from the medium cont...

journal_title：Applied biochemistry and biotechnology

authors： Kim HO,Wee YJ,Kim JN,Yun JS,Ryu HW

更新日期：2006-04-01 00:00:00

abstract：:The recovery of alpha-amylase from the crude enzyme preparation by the reversed micellar liquid-liquid extraction was investigated. The reversed micellar solution was formed by dissolving a cationic surfactant Aliquat 336 in six different alkanes (cyclohexane, n-hexane, isooctane, n-octane, n-decane, and n-dodecane) r...

journal_title：Applied biochemistry and biotechnology

authors： Chang QL,Chen JY

更新日期：1997-02-01 00:00:00

abstract：:For trial use in the local chemotherapy of cancer by a combination of cytosine deaminase (EC 3.5.4.1) and 5-fluorocytosine (J. Biotechnol., (1985), 2, 13-21), 40 U of partially purified cytosine deaminase was obtained from 500 g of commercial compressed bakers' yeast. The enzyme, which is unstable, was immobilized to ...

journal_title：Applied biochemistry and biotechnology

authors： Katsuragi T,Sakai T,Tonomura K

更新日期：1987-09-01 00:00:00

abstract：:A strain of Clostridium thermoaceticum (ATCC 49707) was evaluated for its homoacetate potential. This thermophilic anaerobe best produces acetate from glucose at pH 6.0 and 59 degrees C with a yield of 83% of theoretical. Enzyme hydrolysis of two substrates, a-cellulose and a pulp mill sludge, yielded 68% and 70% dige...

journal_title：Applied biochemistry and biotechnology

authors： Borden JR,Lee YY,Yoon HH

更新日期：2000-04-01 00:00:00

abstract：:An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprot...

journal_title：Applied biochemistry and biotechnology

authors： Batista FR,Greco KN,Astray RM,Jorge SA,Augusto EF,Pereira CA,Mendonça RZ,Moraes AM

更新日期：2011-01-01 00:00:00

abstract：:Label-free detection technique based on impedance was investigated for aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2) analysis in milk products. The impedance change resulting from antigen-antibody interaction was studied using a two-electrode setup made up of silver (Ag) wire. Processed milk such as drinking yogurt and ...

journal_title：Applied biochemistry and biotechnology

authors： Kanungo L,Bacher G,Bhand S

更新日期：2014-10-01 00:00:00

abstract：:A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...

journal_title：Applied biochemistry and biotechnology

authors： Caudan C,Filali A,Lefebvre D,Spérandio M,Girbal-Neuhauser E

更新日期：2012-04-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:Jonesia denitrificans BN-13 produces six xylanases: Xyl1, Xyl2, Xyl3, Xyl4, Xyl5, and Xyl6; the Xyl4 was purified and characterized after two consecutive purification steps using ultrafiltration and anion exchange chromatography. The xylanase-specific activity was found to be 77 unit (U)/mg. The molecular weight of th...

journal_title：Applied biochemistry and biotechnology

authors： Boucherba N,Gagaoua M,Copinet E,Bettache A,Duchiron F,Benallaoua S

更新日期：2014-03-01 00:00:00

abstract：:In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increa...

journal_title：Applied biochemistry and biotechnology

authors： Wu XQ,Wang J,Lü ZR,Tang HM,Park D,Oh SH,Bhak J,Shi L,Park YD,Zou F

更新日期：2010-03-01 00:00:00

abstract：:This work is focused on the biotransformation of R-(+)-limonene and (-)-beta-pinene to bioflavor production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrates. From the isolated microorganisms, 193 were selected in the prescreening using mineral medium for li...

journal_title：Applied biochemistry and biotechnology

authors： Rottava I,Cortina PF,Grando CE,Colla AR,Martello E,Cansian RL,Toniazzo G,Treichel H,Antunes OA,Oestreicher EG,de Oliveira D

更新日期：2010-10-01 00:00:00

abstract：:The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...

journal_title：Applied biochemistry and biotechnology

authors： Thaz CJ,Jayaraman G

更新日期：2014-01-01 00:00:00

abstract：:Dual-responsive poly(N,N-diethylacrylamide) (PDEA) hydrogel films with entrapped horseradish peroxidase (HRP) and glucose oxidase (GOD) were successfully prepared on electrode surface with a simple one-step polymerization procedure under mild conditions, designated as PDEA-HRP-GOD. Cyclic voltammetric (CV) response of...

journal_title：Applied biochemistry and biotechnology

authors： Yao H,Lin L,Wang P,Liu H

更新日期：2014-08-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:We have developed a substrate-induced growth response (SIGR) method for quantifying activity and population dynamics of microorganisms involved in bioremediation processes in soil and bioreactors. The biomass of organisms that can mineralize a given chemical can be estimated based on the concentration of that chemical...

journal_title：Applied biochemistry and biotechnology

authors： Schmidt SK,Colores GM,Hess TF,Radehaus PM

更新日期：1995-07-01 00:00:00

abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

journal_title：Applied biochemistry and biotechnology

authors： Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

更新日期：2014-05-01 00:00:00

abstract：:Nanotechnology is an emerging technology which is an amalgamation of different aspects of science and technology that includes disciplines such as electrical engineering, mechanical engineering, biology, physics, chemistry, and material science. It has potential in the fields of information and communication technolog...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty M,Jain S,Rani V

更新日期：2011-11-01 00:00:00

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not ...

journal_title：Applied biochemistry and biotechnology

authors： Nübel C,Appel B,Hospach I,Mai M,Krasteva N,Nelles G,Petruschka L,Müller S

更新日期：2016-06-01 00:00:00

abstract：:Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...

journal_title：Applied biochemistry and biotechnology

authors： Hinge V,Patil H,Nadaf A

更新日期：2016-02-01 00:00:00

abstract：:Search for efficient low-cost substrate/additives are gaining significant impetus in bacterial cellulose (BC) production. Makgeolli sludge (a traditional Korean wine distillery waste) is enriched with organic acid, alcohol, and sugar. Using makgeolli sludge filtrate (MSF) and Hestrin-Schramm (HS) medium (g/l of distil...

journal_title：Applied biochemistry and biotechnology

authors： Hyun JY,Mahanty B,Kim CG

更新日期：2014-04-01 00:00:00

abstract：:The MixAlco process is a biorefinery that converts lignocellulose into useful chemicals and hydrocarbon fuels via mixed-acid fermentation. For a semi-continuous-staged fermentation train, during each transfer, discrete amounts of material are moved between fermentors and data are tabulated. Because of natural day-to-d...

journal_title：Applied biochemistry and biotechnology

authors： Smith AD,Holtzapple MT

更新日期：2011-04-01 00:00:00

abstract：:The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Jones F,Bailey R,Wilson S,Hiestand J

更新日期：2007-04-01 00:00:00

abstract：:Foot and mouth disease virus, (FMDV) from a crude cell lysate was purified in a single step by affinity chromatography with heparin as a ligand. The virus eluted from an Heparin-Ultrogel A4R column at 1M sodium chloride in 10 mM sodium phosphate buffer, pH 7.0, while most cell protein and albumin did so at lower conce...

journal_title：Applied biochemistry and biotechnology

authors： Navarro del Cañizo AA,Mazza M,Bellinzoni R,Cascone O

更新日期：1996-12-01 00:00:00

abstract：:Fuel ethanol can be produced from softwood through hydrolysis in an enzymatic process. Prior to enzymatic hydrolysis of the softwood, pretreatment is necessary. In this study, two-step steam pretreatment employing dilute H2SO4 impregnation in the first step and SO2 impregnation in the second step, to improve the overa...

journal_title：Applied biochemistry and biotechnology

authors： Söderström J,Pilcher L,Galbe M,Zacchi G

更新日期：2003-04-01 00:00:00

abstract：:An anaerobic sequencing batch reactor containing immobilized biomass (AnSBBR) was used to produce biomethane by treating the effluent from another AnSBBR used to produce biohydrogen from glucose- (AR-EPHG) and sucrose-based (AR-EPHS) wastewater. In addition, biomethane was also produced from sucrose-based synthetic wa...

journal_title：Applied biochemistry and biotechnology

authors： Lullio TG,Souza LP,Ratusznei SM,Rodrigues JA,Zaiat M

更新日期：2014-11-01 00:00:00

abstract：:In this study, Nostoc muscorum, a native cyanobacterial species isolated from a coal mining site, was employed to remove Cu(II), Zn(II), Pb(II) and Cd(II) from aqueous solution containing these metals in the mixture. In this multicomponent study, carried out as per the statistically valid Plackett-Burman design of exp...

journal_title：Applied biochemistry and biotechnology

authors： Roy AS,Hazarika J,Manikandan NA,Pakshirajan K,Syiem MB

更新日期：2015-04-01 00:00:00

abstract：:Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

journal_title：Applied biochemistry and biotechnology

authors： Ximenes EA,Brandon SK,Doran-Peterson J

更新日期：2008-03-01 00:00:00