Implantable enzyme capsules for cancer chemotherapy from bakers' yeast cytosine deaminase immobilized on epoxy-acrylic resin and urethane prepolymer.

abstract：:The glpX gene from Francisella tularensis encodes for the class II fructose 1,6-bisphosphatase (FBPaseII) enzyme. The glpX gene has been verified to be essential in F. tularensis, and the inactivation of this gene leads to impaired bacterial growth on gluconeogenic substrates. In the present work, we have complemented...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Wolf NM,Bondoc JMG,Movahedzadeh F

更新日期：2017-12-01 00:00:00

abstract：:A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been d...

journal_title：Applied biochemistry and biotechnology

authors： Wu Q,Chen CX,Du LL,Lin XF

更新日期：2010-04-01 00:00:00

abstract：:The effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Vitex trifolia. Multiple shoots were induced in nodal explants obtained from a mature tree on Murashige and Skoog (MS) medium ...

journal_title：Applied biochemistry and biotechnology

authors： Ahmed MR,Anis M

更新日期：2012-11-01 00:00:00

abstract：:An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination ...

journal_title：Applied biochemistry and biotechnology

authors： Fatima N,Ahmad N,Ahmad I,Anis M

更新日期：2015-09-01 00:00:00

abstract：:Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol ferment...

journal_title：Applied biochemistry and biotechnology

authors： Sun L,Wang L,Chen H

更新日期：2020-10-01 00:00:00

abstract：:This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

journal_title：Applied biochemistry and biotechnology

authors： Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

更新日期：2000-11-01 00:00:00

abstract：:Androstenedione (AD) is a steroid intermediate used in the pharmaceutical industry for the production of several important anabolic drugs. An important route for producing AD is by the microbial transformation of sterols. Microbes capable of efficiently transforming sterols to AD are few and newer strains need to be i...

journal_title：Applied biochemistry and biotechnology

authors： Malaviya A,Gomes J

更新日期：2009-08-01 00:00:00

abstract：:This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

journal_title：Applied biochemistry and biotechnology

authors： Sun ZL,Sun LQ,Chen GZ

更新日期：2019-04-01 00:00:00

abstract：:Euryale ferox Salisbury is an important aquatic food plant cultivated largely in eastern India. E. ferox is a monotypic genus, and breeding programmes have mostly relied on the variability present in the primary gene pool. Knowledge of the genetic structure of the population is limited, and there are very few reports ...

journal_title：Applied biochemistry and biotechnology

authors： Kumar H,Priya P,Singh N,Kumar M,Choudhary BK,Kumar L,Singh IS,Kumar N

更新日期：2016-12-01 00:00:00

abstract：:Oil palm empty fruit bunch (EFB) is abundantly available in Malaysia and it is a potential source of xylose for the production of high-value added products. This study aimed to optimize the hydrolysis of EFB using dilute sulfuric acid (H2SO4) and phosphoric acid (H3PO4) via response surface methodology for maximum xyl...

journal_title：Applied biochemistry and biotechnology

authors： Tan HT,Dykes GA,Wu TY,Siow LF

更新日期：2013-08-01 00:00:00

abstract：:Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectrosc...

journal_title：Applied biochemistry and biotechnology

authors： Türkhan A,Kaya ED,Koçyiğit A

更新日期：2020-10-01 00:00:00

abstract：:A novel mannan-specific lectin was isolated from the roots of a traditional Chinese herbal medicine, Ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. With a molecular mass of 19,835.7 Da demonstrated by MALDI-TOF analysis, this novel agglutinin was designated as O. pedunculosum aggluti...

journal_title：Applied biochemistry and biotechnology

authors： He XM,Ji N,Xiang XC,Luo P,Bao JK

更新日期：2011-12-01 00:00:00

abstract：:Potassium acetate is currently made by reacting petroleum-based acetic acid with potassium hydroxide. An alternate process, anaerobic fermentation of dextrose with Clostridium thermoaceticum, could be used and could possibly be cheaper. Growth characteristics and productivity of the fermentation were optimized to maxi...

journal_title：Applied biochemistry and biotechnology

authors： Shah MM,Akanbi F,Cheryan M

更新日期：1997-04-01 00:00:00

abstract：:The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompeti...

journal_title：Applied biochemistry and biotechnology

authors： Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

更新日期：2012-01-01 00:00:00

abstract：:S-Adenosylhomocysteine hydrolase (SAHase) encoded by sahase gene is a determinant when catalyzing the reversible conversion of adenosine and homocysteine to S-adenosylhomocysteine in most living organisms. The sahase gene was isolated from the genome of the highly thermostable anaerobic bacteria Thermotoga maritima, a...

journal_title：Applied biochemistry and biotechnology

authors： Lozada-Ramírez JD,Sánchez-Ferrer A,García-Carmona F

更新日期：2013-06-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:Environmental conditions affect the production of extracellular polysaccharide by Azotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored expopolysaccharide production, which reache...

journal_title：Applied biochemistry and biotechnology

authors： De la Vega MG,Cejudo FJ,Paneque A

更新日期：1991-09-01 00:00:00

abstract：:Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This ...

journal_title：Applied biochemistry and biotechnology

authors： Jonas R,Silveira MM

更新日期：2004-07-01 00:00:00

abstract：:A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...

journal_title：Applied biochemistry and biotechnology

authors： Webb OF,Phelps TJ,Bienkowski PR,DiGrazia PM,Reed GD,Applegate B,White DC,Sayler GS

更新日期：1991-04-01 00:00:00

abstract：:The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

journal_title：Applied biochemistry and biotechnology

authors： Sousa CA,Hanselaer S,Soares EV

更新日期：2015-01-01 00:00:00

abstract：:The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obta...

journal_title：Applied biochemistry and biotechnology

authors： Manni L,Jellouli K,Ghorbel-Bellaaj O,Agrebi R,Haddar A,Sellami-Kamoun A,Nasri M

更新日期：2010-04-01 00:00:00

abstract：:A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

journal_title：Applied biochemistry and biotechnology

authors： Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

更新日期：2008-04-01 00:00:00

abstract：:Pretreatment based on aqueous ammonia was investigated under two different modes of operation: soaking in aqueous ammonia and ammonia recycle percolation. These processes were applied to three different feedstocks with varied composition: corn stover, high lignin (HL), and low lignin (LL) hybrid poplars. One of the im...

journal_title：Applied biochemistry and biotechnology

authors： Gupta R,Kim TH,Lee YY

更新日期：2008-03-01 00:00:00

abstract：:The native Celluclast BG cellulase enzyme complex consists of different enzymes which can also degrade great substrate molecules as native celluloses. This enzyme complex has been covered by a very thin, a few nanometers thick, polymer layer, in order to improve its stability. It has been proved that the polymer layer...

journal_title：Applied biochemistry and biotechnology

authors： Hegedüs I,Hancsók J,Nagy E

更新日期：2012-11-01 00:00:00

abstract：:Ethanol from corn is produced using dry grind corn process in which simultaneous saccharification and fermentation (SSF) is one of the most critical unit operations. In this work an optimal controller based on a previously validated SSF model was developed by formulating the SSF process as a Bolza problem and using gr...

journal_title：Applied biochemistry and biotechnology

authors： Murthy GS,Johnston DB,Rausch KD,Tumbleson ME,Singh V

更新日期：2012-01-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00

abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

journal_title：Applied biochemistry and biotechnology

authors： Tadolini B,Hakim G

更新日期：1981-09-01 00:00:00

abstract：:The feasibility of using mixtures of C4 and C5 chain-length aliphatic alcohols from fusel oil as the bulk organic media for lipase-mediated synthesis of laurate esters was assessed. Reaction mixtures consisted of lauric acid, lipase, solvent (if added), and appropriate amount of fusel oil (previously dehydrated with i...

journal_title：Applied biochemistry and biotechnology

authors： De Castro HF,Moriya RY,Oliveira PC,Soares CM

更新日期：1999-04-01 00:00:00

abstract：:Thermoacidophiles are microorganisms capable of optimum growth under a combination of high temperature and low pH. These microorganisms are a rich source of thermo- and acid- active/stable glycosyl hydrolases. Such enzymes could find use as novel biocatalysts in industrial processes, as operation at elevated temperatu...

journal_title：Applied biochemistry and biotechnology

authors： Murphy J,Ryan MP,Walsh G

更新日期：2020-07-01 00:00:00

abstract：:In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

journal_title：Applied biochemistry and biotechnology

authors： Seidi A,Mie M,Kobatake E

更新日期：2009-08-01 00:00:00