Heterologous Acidothermus cellulolyticus 1,4-beta-endoglucanase E1 produced within the corn biomass converts corn stover into glucose.

Abstract:

:Commercial conversion of lignocellulosic biomass to fermentable sugars requires inexpensive bulk production of biologically active cellulase enzymes, which might be achieved through direct production of these enzymes within the biomass crops. Transgenic corn plants containing the catalytic domain of Acidothermus cellulolyticus E1 endo-1,4-beta glucanase and the bar bialaphos resistance coding sequences were generated after Biolistic (BioRad Hercules, CA) bombardment of immature embryo-derived cells. E1 sequences were regulated under the control of the cauliflower mosaic virus 35S promoter and tobacco mosaic virus translational enhancer, and E1 protein was targeted to the apoplast using the signal peptide of tobacco pathogenesis-related protein to achieve accumulation of this enzyme. The integration, expression, and segregation of E1 and bar transgenes were demonstrated, respectively, through Southern and Western blotting, and progeny analyses. Accumulation of up to 1.13% of transgenic plant total soluble proteins was detected as biologically active E1 by enzymatic activity assay. The corn-produced heterologous E1 could successfully convert ammonia fiber explosion-pretreated corn stover polysaccharides into glucose as a fermentable sugar for ethanol production, confirming that the E1 enzyme is produced in its active form.

authors

Ransom C,Balan V,Biswas G,Dale B,Crockett E,Sticklen M

doi

10.1007/s12010-007-9053-3

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

207-19

issue

1-12

eissn

0273-2289

issn

1559-0291

journal_volume

137-140

pub_type

杂志文章
  • Evaluation of Lighting Systems, Carbon Sources, and Bacteria Cultures on Photofermentative Hydrogen Production.

    abstract::Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2655-5

    authors: Hu C,Choy SY,Giannis A

    更新日期:2018-05-01 00:00:00

  • Adenosine Monophosphate-Activated Protein Kinase (AMPK) as a Diverse Therapeutic Target: A Computational Perspective.

    abstract::Adenosine monophosphate-activated protein kinase (AMPK) is viewed as a privileged therapeutic target for several diseases such as cancer, diabetes, inflammation, obesity, etc. In addition, AMPK has entered the limelight of current drug discovery with its evolution as a key metabolic regulator. AMPK also plays a key ro...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-015-1911-9

    authors: Ramesh M,Vepuri SB,Oosthuizen F,Soliman ME

    更新日期:2016-02-01 00:00:00

  • Identification of potential markers related to neoadjuvant chemotherapy sensitivity of breast cancer by SELDI-TOF MS.

    abstract::Neoadjuvant chemotherapy (NACT) is known to be beneficial for patients with locally advanced breast cancer. However, there is still no unified standard on the evaluation of NACT. To identify the potential markers related to NACT sensitivity of breast cancer, in the present study, we examined the protein spectrum of br...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9464-z

    authors: Zhang K,Yuan K,Wu H,Li Q,Wang Y,Chen S,Zhang L,Gu H,Fu R

    更新日期:2012-02-01 00:00:00

  • Determining yields in high solids enzymatic hydrolysis of biomass.

    abstract::As technologies for utilizing biomass for fuel and chemical production continue to improve, enzymatic hydrolysis can be run at still higher solids concentrations. For hydrolyses that initially contain little or no free water (10-40% total solids, w/w), the saccharification of insoluble polymers into soluble sugars inv...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8375-0

    authors: Kristensen JB,Felby C,Jørgensen H

    更新日期:2009-05-01 00:00:00

  • Prevalence of genetically modified rice, maize, and soy in Saudi food products.

    abstract::Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified org...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0405-x

    authors: Elsanhoty RM,Al-Turki AI,Ramadan MF

    更新日期:2013-10-01 00:00:00

  • Construction of a genetically engineered microorganism that simultaneously degrades organochlorine and organophosphate pesticides.

    abstract::Field contamination with pesticide mixtures of organophosphates (OPs) and organochlorines (OCs) is becoming global issues to be solved urgently. The strategy of utilizing engineered microorganisms that have an ability to simultaneously degrade OPs and OCs has increasingly received great interest. In this work, an OP d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9450-5

    authors: Yang J,Liu R,Song W,Yang Y,Cui F,Qiao C

    更新日期:2012-02-01 00:00:00

  • Shoot organogenesis from root-derived callus of Rhinacanthus nasutus (L.) Kurz. and assessment of clonal fidelity of micropropagted plants using RAPD analysis.

    abstract::An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0598-z

    authors: Cheruvathur MK,Thomas TD

    更新日期:2014-02-01 00:00:00

  • Monitoring of TGF-β 1-Induced Human Lung Adenocarcinoma A549 Cells Epithelial-Mesenchymal Transformation Process by Measuring Cell Adhesion Force with a Microfluidic Device.

    abstract::The epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties. It is believed that EMT is associated with initiation and completion of the invasion-metastasis cascade. In this study, an economic approach wa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1862-1

    authors: Li Y,Gao A,Yu L

    更新日期:2016-01-01 00:00:00

  • Effects of oxygen limitation on xylose fermentation, intracellular metabolites, and key enzymes of Neurospora crassa AS3.1602.

    abstract::The effects of oxygen limitation on xylose fermentation of Neurospora crassa AS3.1602 were studied using batch cultures. The maximum yield of ethanol was 0.34 g/g at oxygen transfer rate (OTR) of 8.4 mmol/L.h. The maximum yield of xylitol was 0.33 g/g at OTR of 5.1 mmol/L.h. Oxygen limitation greatly affected mycelia ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8038-6

    authors: Zhang Z,Qu Y,Zhang X,Lin J

    更新日期:2008-03-01 00:00:00

  • Laboratory method for high-solids countercurrent fermentations.

    abstract::Equipment and procedures were developed to study the conversion of lignocellulosic biomass to carboxylic acids using high-solids countercurrent fermentations. Countercurrent fermentations of cattle manure yielded a rapid fermentation (maximum 2.98 g of total acid/[L x d]) with high acid concentrations (maximum of 32.5...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:94:2:111

    authors: Ross MK,Holtzapple MT

    更新日期:2001-05-01 00:00:00

  • Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system.

    abstract::A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipett...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:91-93:1-9:109

    authors: Sato R,Takeyama H,Tanaka T,Matsunaga T

    更新日期:2001-04-01 00:00:00

  • ABCC subfamily vacuolar transporters are involved in Pb (lead) detoxification in Saccharomyces cerevisiae.

    abstract::The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1252-0

    authors: Sousa CA,Hanselaer S,Soares EV

    更新日期:2015-01-01 00:00:00

  • Aerosol-assisted plasma deposition of hydrophobic polycations makes surfaces highly antimicrobial.

    abstract::The currently used multistep chemical synthesis for making surfaces antimicrobial by attaching to them hydrophobic polycations is replaced herein by an aerosol-assisted plasma deposition procedure. To this end, N,N-hexyl,methyl-PEI (HMPEI) is directly plasma-coated onto a glass surface. The resultant immobilized HMPEI...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0593-4

    authors: Liu H,Kim Y,Mello K,Lovaasen J,Shah A,Rice N,Yim JH,Pappas D,Klibanov AM

    更新日期:2014-02-01 00:00:00

  • New Insights on Coffea miRNAs: Features and Evolutionary Conservation.

    abstract::Small RNAs influence the gene expression at the post-transcriptional level by guiding messenger RNA (mRNA) cleavage, translational repression, and chromatin modifications. In addition to model plants, the microRNAs (miRNAs) have been identified in different crop species. In this work, we developed a specific pipeline ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1785-x

    authors: Chaves SS,Fernandes-Brum CN,Silva GF,Ferrara-Barbosa BC,Paiva LV,Nogueira FT,Cardoso TC,Amaral LR,de Souza Gomes M,Chalfun-Junior A

    更新日期:2015-10-01 00:00:00

  • Sphaerotilus natans isolated from activated sludge and its production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate).

    abstract::Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:98-100:1-9:1061

    authors: Liu K,Chua H,Lo WH,Lawford H,Yu PH

    更新日期:2002-04-01 00:00:00

  • Global Transcriptome Profiling of Enterobacter Strain NRS-1 in Response to Hydrogen Peroxide Stress Treatment.

    abstract::Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in resp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03313-x

    authors: Fei YY,Bhat JA,Gai JY,Zhao TJ

    更新日期:2020-08-01 00:00:00

  • Effect of a nonmetabolizable analog of fructose-1,6-bisphosphate on glycolysis and ethanol production in strains of Saccharomyces cerevisiae and Escherichia coli.

    abstract::Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02729071

    authors: Nghiem NP,Cofer TM

    更新日期:2007-05-01 00:00:00

  • Characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I and its application to PCR.

    abstract::A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0918-y

    authors: Seo KJ,Cho SS,Ppyun HW,Youn MH,Kim SH,Seo BS,Kwon ST

    更新日期:2014-07-01 00:00:00

  • Affinity-based separations and purifications. Patents and literature.

    abstract::The separation and purification of biologically functional molecules (e.g., proteins, antibodies, peptides, hormones, low molecular weight biologicals) is of fundamental importance to biotechnology. Affinity separations have become a particularly attractive method for bioseparations due to their high degree of selecti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02921518

    authors: Dordick JS

    更新日期:1991-01-01 00:00:00

  • Transesterification of Waste Frying Oil and Soybean Oil by Combi-lipases Under Ultrasound-Assisted Reactions.

    abstract::This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was perform...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2763-x

    authors: Poppe JK,Matte CR,Fernandez-Lafuente R,Rodrigues RC,Ayub MAZ

    更新日期:2018-11-01 00:00:00

  • Biodiesel residual glycerol metabolism by Klebsiella pneumoniae: pool of metabolites under anaerobiosis and oxygen limitation as a function of feeding rates.

    abstract::The metabolism of residual glycerol from biodiesel synthesis by Klebsiella pneumoniae BLh-1 was investigated in this study. Batch and fed-batch cultivations were performed in bioreactors under anaerobic and oxygen limitation conditions. Results of batch cultivations showed that the main product was 1,3-propanediol (1,...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0114-5

    authors: Rossi DM,de Souza EA,Ayub MA

    更新日期:2013-03-01 00:00:00

  • White rot fungus Inonotus obliquus pretreatment to improve tran-1,4-polyisoprene extraction and enzymatic saccharification of Eucommia ulmoides leaves.

    abstract::This study proposes an innovative strategy of lignocellulose biodegradation by Inonotus obliquus under solid-state fermentation in extracting Eucommia ulmoides trans-1,4-polyisoprene (EUG) and producing reducing sugars efficiently. EUG and sugars were obtained through the white rot fungal pretreatment of E. ulmoides l...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03347-1

    authors: Qian S,Zhang C,Zhu Z,Huang P,Xu X

    更新日期:2020-11-01 00:00:00

  • High-yield hypocrellin A production in solid-state fermentation by Shiraia sp. SUPER-H168.

    abstract::Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8728-3

    authors: Cai Y,Liang X,Liao X,Ding Y,Sun J,Li X

    更新日期:2010-04-01 00:00:00

  • Tannase production by Penicillium purpurogenum PAF6 in solid state fermentation of tannin-rich plant residues following OVAT and RSM.

    abstract::Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the mo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9547-5

    authors: Jana A,Maity C,Halder SK,Mondal KC,Pati BR,Mohapatra PK

    更新日期:2012-07-01 00:00:00

  • Continuous production of butanol from starch-based packing peanuts.

    abstract::Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and final starch concentrations were 69.6 and 11.1 g/L, respectively. I...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:106:1-3:375

    authors: Ezeji TC,Groberg M,Qureshi N,Blaschek HP

    更新日期:2003-04-01 00:00:00

  • Signal amplification by substrate recycling on polyaniline/lactate oxidase/lactate dehydrogenase bienzyme electrodes.

    abstract::The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:96:1-3:239

    authors: Chaubey A,Pande KK,Pandey MK,Singh VS

    更新日期:2001-10-01 00:00:00

  • Purification and biochemical characterization of a highly thermostable xylanase from Actinomadura sp. strain Cpt20 isolated from poultry compost.

    abstract::An extracellular thermostable xylanase from a newly isolated thermophilic Actinomadura sp. strain Cpt20 was purified and characterized. Based on matrix-assisted laser desorption-ionization time-of-flight mass spectrometry analysis, the purified enzyme is a monomer with a molecular mass of 20,110.13 Da. The 19 residue ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9457-y

    authors: Taibi Z,Saoudi B,Boudelaa M,Trigui H,Belghith H,Gargouri A,Ladjama A

    更新日期:2012-02-01 00:00:00

  • Environmental effects on transglutaminase production and cell sporulation in submerged cultivation of Bacillus circulans.

    abstract::In this research, the effects of pH, temperature, and oxygen on growth kinetics of a newly isolated strain of Bacillus circulans from the Amazon and their correlations with transglutaminase (TGase) production and cell sporulation were investigated. Statistical experimental methods were used to optimize these parameter...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8338-5

    authors: de Souza CF,de Matos GS,Flôres SH,Ayub MA

    更新日期:2009-08-01 00:00:00

  • Novel approaches to the purification of penicillin acylase.

    abstract::Penicillin acylase of E. coli NCIM 2400 has been purified to homogeneity using a combination of hydrophobic interaction chromatography and DEAE-cellulose treatment. A variety of substituted matrices were synthesized using D- or DL-phenylglycine, norleucine, ampicillin, or amoxycillin as ligands, all of which retained ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798397

    authors: Mahajan PB,Borkar PS

    更新日期:1984-10-01 00:00:00

  • Characteristics of oxidation-reduction potential, VFAs, SCOD, N, and P in an ATAD system under different thermophilic temperatures.

    abstract::One-stage autoheated thermophilic aerobic digestion (ATAD) can stabilize sludge to meet class A standard. In this study, batch experiments were conducted to investigate the characteristics of oxidation-reduction potential (ORP), volatile fatty acids (VFAs), soluble chemical oxygen demand (SCOD), and nutrients at diffe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1241-3

    authors: Cheng J,Kong F,Zhu J,Wu X

    更新日期:2015-01-01 00:00:00