Immunoassay for native enzyme quantification in biological samples.

abstract：:Over the last two decades many strategies have been planned to design specific drugs for rare diseases to target their action at the DNA level. Advancements in our understanding of the interactions of small nonpeptide molecules with DNA have opened the doors for "rational" drug design. Special methods have now been de...

journal_title：Applied biochemistry and biotechnology

authors： Prabhakar P,Kayastha AM

更新日期：1994-04-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:The dye decolorization potential of the white-rot fungus Phlebia brevispora TMIC33929 when grown alone or in coculture with its growth-promoting bacterium Enterobacter sp. TN3W-14 was evaluated in low nitrogen liquid medium at different pHs. Axenic fungus removed a similar amount of Congo red and crystal violet at pH ...

journal_title：Applied biochemistry and biotechnology

authors： Harry-Asobara JL,Kamei I

更新日期：2019-12-01 00:00:00

abstract：:This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...

journal_title：Applied biochemistry and biotechnology

authors： Peixoto G,Pantoja-Filho JL,Agnelli JA,Barboza M,Zaiat M

更新日期：2012-10-01 00:00:00

abstract：:The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denatur...

journal_title：Applied biochemistry and biotechnology

authors： Wu Z,Dong H,Zou L,Lu D,Liu Z

更新日期：2011-08-01 00:00:00

abstract：:6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of D-glucose. They serve as precursors of the antimicrobial α-methylene-γ-butyrolactones tulipalins A (PaA) and B (PaB). The conversions of 6-PosA/6-PosB to PaA/PaB are catalyz...

journal_title：Applied biochemistry and biotechnology

authors： Nomura T,Ogita S,Kato Y

更新日期：2019-05-01 00:00:00

abstract：:The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, a...

journal_title：Applied biochemistry and biotechnology

authors： Kálmán G,Recseg K,Gáspár M,Réczey K

更新日期：2006-03-01 00:00:00

abstract：:To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Ishii M,Junior AP,de Oliveira Nascimento L,de Souza LC,Cholewa O

更新日期：2004-04-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:Pretreatment based on aqueous ammonia was investigated under two different modes of operation: soaking in aqueous ammonia and ammonia recycle percolation. These processes were applied to three different feedstocks with varied composition: corn stover, high lignin (HL), and low lignin (LL) hybrid poplars. One of the im...

journal_title：Applied biochemistry and biotechnology

authors： Gupta R,Kim TH,Lee YY

更新日期：2008-03-01 00:00:00

abstract：:Thermomucor indicae-seudaticae, a glucoamylase-producing thermophilic mould, was mutagenised using nitrous acid and gamma ((60)Co) irradiation in a sequential manner to isolate deregulated mutants for enhanced production of glucoamylase. The mutants were isolated on Emerson YpSs agar containing a non-metabolisable glu...

journal_title：Applied biochemistry and biotechnology

authors： Kumar P,Satyanarayana T

更新日期：2009-07-01 00:00:00

abstract：:The potential market for lactic acid as the feedstock for biodegradable polymers, oxygenated chemicals, and specialty chemicals is significant. L-lactic acid is often the desired enantiomer for such applications. However, stereospecific lactobacilli do not metabolize starch efficiently. In this work, Argonne researche...

journal_title：Applied biochemistry and biotechnology

authors： Tsai SP,Moon SH

更新日期：1998-04-01 00:00:00

abstract：:The increased additive amount of adjuncts in the raw materials of Chinese beer requires the usage of protease to release more water-soluble proteins. Here, a metallo-neutral protease suited for brewing industry was purified from Bacillus amyloliquefaciens SYB-001. A 5.6-fold purification of the neutral protease was ac...

journal_title：Applied biochemistry and biotechnology

authors： Wang J,Xu A,Wan Y,Li Q

更新日期：2013-08-01 00:00:00

abstract：:Events from the past growth conditions influence the course of physiology, and it gets reflected in the present cell behaviour. During this process, cells acquire a metabolic option which carries signature of the past, and it dictates the performance in the present situation. Study uses Escherichia coli as a sample or...

journal_title：Applied biochemistry and biotechnology

authors： Puranik S,Purohit HJ

更新日期：2014-11-01 00:00:00

abstract：:A novel β-1,3-glucanase gene, designated Ccglu17A, was cloned from the biological control fungus Chaetomium cupreum Ame. Its 1626-bp open reading frame encoded 541 amino acids. The corresponding amino acid sequence showed highest identity (67 %) with a glycoside hydrolase family 17 β-1,3-glucanase from Chaetomium glob...

journal_title：Applied biochemistry and biotechnology

authors： Jiang C,Song J,Cong H,Zhang J,Yang Q

更新日期：2017-05-01 00:00:00

abstract：:Production costs of cellulosic biofuels can be lowered if cellulases are recovered and reused using particulate carriers that can be extracted after biomass hydrolysis. Such enzyme recovery was recently demonstrated using enzymogel nanoparticles with grafted polymer brushes loaded with cellulases. In this work, cellul...

journal_title：Applied biochemistry and biotechnology

authors： Samaratunga A,Kudina O,Nahar N,Zakharchenko A,Minko S,Voronov A,Pryor SW

更新日期：2015-06-01 00:00:00

abstract：:Lipase-catalyzed esterification of selected phenolic acids with linolenyl alcohols was investigated in selected organic solvent media. The enzyme activity for the esterification of dihydrocaffeic acid with linolenyl alcohol in solvent mixtures of hexane/2-butanone of 75:25 (v/v) and 65:35 (v/v) was 0.88 and 0.47 micro...

journal_title：Applied biochemistry and biotechnology

authors： Sabally K,Karboune S,Yeboah FK,Kermasha S

更新日期：2005-10-01 00:00:00

abstract：:A mesophilic bacterial culture producing a novel thermostable alkaline lipase was isolated from oil rich soil sample and identified as Bacillus subtilis EH 37. The lipase was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography with 17.8-fold purification and 41.9 U/ml specif...

journal_title：Applied biochemistry and biotechnology

authors： Ahmed EH,Raghavendra T,Madamwar D

更新日期：2010-04-01 00:00:00

abstract：:RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not ...

journal_title：Applied biochemistry and biotechnology

authors： Nübel C,Appel B,Hospach I,Mai M,Krasteva N,Nelles G,Petruschka L,Müller S

更新日期：2016-06-01 00:00:00

abstract：:This study aimed to evaluate the influence of the culture medium supplementation with mineral ions, focusing on the growth of Streptococcus zooepidemicus as well as on the production and average molecular weight (MW) of hyaluronic acid (HA). The ions were investigated in terms of individual absence from the totally su...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Eguchi SY,Santana MH

更新日期：2010-12-01 00:00:00

abstract：:Bioreduction of 1-phenyl-2-propanone to prepare (S)-1-phenyl-2-propanol, a useful pharmaceutical intermediate, was performed with growing cells of Rhodococcus erythropolis JX-021, giving 14 mM (1.9 g/L) product in 99% e.e. at 5 h in the catalysis of 15 mM substrate. The reduction stopped afterwards due to strong inhib...

journal_title：Applied biochemistry and biotechnology

authors： Jin JZ,Li H,Zhang J

更新日期：2010-11-01 00:00:00

abstract：:Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

journal_title：Applied biochemistry and biotechnology

authors： Rondags E,Halliday E,Marc I

更新日期：1998-02-01 00:00:00

abstract：:Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified org...

journal_title：Applied biochemistry and biotechnology

authors： Elsanhoty RM,Al-Turki AI,Ramadan MF

更新日期：2013-10-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The p...

journal_title：Applied biochemistry and biotechnology

authors： Shivlata L,Satyanarayana T

更新日期：2017-04-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs which play significant role in RNA interference. The present work deals with mining of the conserved miRNA and their target genes from the contigs, ESTs, and BAC end sequences of commercially important catfish, Clarias batrachus, from India. A total of 138, 1 and 1 conserved pre-miR...

journal_title：Applied biochemistry and biotechnology

authors： Agarwal S,Nagpure NS,Srivastava P,Kumar R,Pandey M,Srivastava S,Jena JK,Das P,Kushwaha B

更新日期：2017-07-01 00:00:00

abstract：:The main goal of this paper was to elaborate the possibility of industrial application of biosorption properties of Enteromorpha prolifera (production of mineral feed additives for livestock). In this study, biosorption process was used in the binding of chromium(III) ions from aqueous solution by the green macroalga....

journal_title：Applied biochemistry and biotechnology

authors： Michalak I,Chojnacka K

更新日期：2010-03-01 00:00:00

abstract：:A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...

journal_title：Applied biochemistry and biotechnology

authors： Seo KJ,Cho SS,Ppyun HW,Youn MH,Kim SH,Seo BS,Kwon ST

更新日期：2014-07-01 00:00:00

abstract：:In our previous study, chitosan-alginate microcapsules were developed to protect egg yolk immunoglobulin (IgY) from gastric inactivation. The present study was undertaken to determine the effect of chitosan concentration (0-0.8%; w/v) on various properties of the microcapsules in order to produce the optimum chitosan-...

journal_title：Applied biochemistry and biotechnology

authors： Li XY,Jin LJ,Lu YN,Zhen YH,Li SY,Wang LH,Xu YP

更新日期：2009-12-01 00:00:00

abstract：:The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

journal_title：Applied biochemistry and biotechnology

authors： Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

更新日期：2019-10-01 00:00:00