Abstract：
:This study aimed to evaluate the influence of the culture medium supplementation with mineral ions, focusing on the growth of Streptococcus zooepidemicus as well as on the production and average molecular weight (MW) of hyaluronic acid (HA). The ions were investigated in terms of individual absence from the totally supplemented medium (C+) or individual presence in the non-supplemented medium (C-), where C+ and C- were used as controls. Differences between the effects were analyzed using the Tukey's test at p < 0.05. The adopted criteria considered required the ions, whose individual absence attained at 80% or less of the C+ and their individual presence was 20% or more than the C-. The supplementation was either inhibitory or acted in synergy with other ions, when the individual absence or presence was 20% higher than C+ or 20% lower than C-, respectively. Results showed that the effects of C+ or C- were equal for both the production of HA and its yield from glucose. However, C+ showed to be beneficial to cell growth while the individual absence of Na+ was beneficial to the production of HA. The highest MW of HA (7.4 x 10⁷ Da) was observed in the individual presence of Na+ in spite of the lowest HA concentration (0.65 g x L⁻¹). These results suggest that the quality of HA can be modulated through the mineral ion supplementation.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Pires AM,Eguchi SY,Santana MHdoi
10.1007/s12010-010-8987-zsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
2125-35issue
8eissn
0273-2289issn
1559-0291journal_volume
162pub_type
杂志文章abstract：:Mycobacterium avium subsp. paratuberculosis heat shock protein 70 (MAPHsp70) is an immunodominant antigen, which can be used as a subunit vaccine against bovine paratuberculosis. In the present study, we evaluated the immunogenic activities of MAPHsp70 expressed by DNA vaccine in chicken and the use of prepared specif...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-012-9648-1
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-019-03165-0
更新日期：2020-09-01 00:00:00
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doi：10.1007/s12010-020-03484-7
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-020-03276-z
更新日期：2020-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-017-2625-y
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journal_title：Applied biochemistry and biotechnology
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02920453
更新日期：1997-04-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章,评审
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更新日期：1991-12-01 00:00:00
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更新日期：2017-04-01 00:00:00
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更新日期：2014-11-01 00:00:00
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doi：10.1007/s12010-008-8330-0
更新日期：2009-07-01 00:00:00
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更新日期：2005-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-011-9332-x
更新日期：2011-11-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0598-z
更新日期：2014-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-013-0235-x
更新日期：2013-07-01 00:00:00
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更新日期：2013-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-08-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2015-04-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/BF02798431
更新日期：1987-03-01 00:00:00
