Engineering plant alternative oxidase function in mammalian cells: substitution of the motif-like sequence ENV for QDT diminishes catalytic activity of Arum concinnatum AOX1a expressed in HeLa cells.

abstract：:Thermomucor indicae-seudaticae, a glucoamylase-producing thermophilic mould, was mutagenised using nitrous acid and gamma ((60)Co) irradiation in a sequential manner to isolate deregulated mutants for enhanced production of glucoamylase. The mutants were isolated on Emerson YpSs agar containing a non-metabolisable glu...

journal_title：Applied biochemistry and biotechnology

authors： Kumar P,Satyanarayana T

更新日期：2009-07-01 00:00:00

abstract：:Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process ...

journal_title：Applied biochemistry and biotechnology

authors： Stanescu MD,Fogorasi M,Shaskolskiy BL,Gavrilas S,Lozinsky VI

更新日期：2010-04-01 00:00:00

abstract：:Kariba weed (Salvinia molesta) was used as biomass feedstock for ethanol production by separate hydrolysis and fermentation (SHF). Monosaccharides from Kariba weed hydrolysate were produced using thermal acid hydrolysis, sonication, and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis of ...

journal_title：Applied biochemistry and biotechnology

authors： Kityo MK,Sunwoo I,Kim SH,Park YR,Jeong GT,Kim SK

更新日期：2020-09-01 00:00:00

abstract：:Endoglucanase 5 (EG5) has been isolated from the strain of E. coli TG1 harboring recombinant plasmid pCU108, which contains the cel5 gene of C. thermocellum. The enzyme has been produced with 98-fold purification and a final yield of 27% by using subsequent twofold high performance ion-exchange chromatography on Mono ...

journal_title：Applied biochemistry and biotechnology

authors： Mosolova TP,Kalyuzhnyi SV,Varfolomeyev SD,Velikodvorskaya GA

更新日期：1993-07-01 00:00:00

abstract：:Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In the present study, the kinetic assay in air-saturated solutions and the kinetic behavior of PO from Pieris rapae (Lepidoptera) larvae ...

journal_title：Applied biochemistry and biotechnology

authors： Xue CB,Luo WC,Jiang L,Xie XY,Xiao T,Yan L

更新日期：2007-11-01 00:00:00

abstract：:To screen strains of halotolerant or halophile bacteria which are able to convert isoeugenol to vanillin, 36 different strains of bacteria isolated from the salty environments in Iran were investigated. During growth on isoeugenol, a moderately halotolerant Gram-negative coccobacil showed capability of converting isoe...

journal_title：Applied biochemistry and biotechnology

authors： Ashengroph M,Nahvi I,Zarkesh-Esfahani H,Momenbeik F

更新日期：2012-01-01 00:00:00

abstract：:The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...

journal_title：Applied biochemistry and biotechnology

authors： Errasti ME,Natalucci CL,Caffini NO,Rotelli AE,Brullo A,Maras B,Trejo SA,López LMI

更新日期：2018-09-01 00:00:00

abstract：:An endo-beta-1,4-xylanase-encoding gene, xyn11NX, was cloned from Nesterenkonia xinjiangensis CCTCC AA001025 and expressed in Escherichia coli. The gene encoded a 192-amino acid polypeptide and a putative 50-amino acid signal peptide. The deduced amino acid sequence exhibited a high degree of similarity with the xylan...

journal_title：Applied biochemistry and biotechnology

authors： Kui H,Luo H,Shi P,Bai Y,Yuan T,Wang Y,Yang P,Dong S,Yao B

更新日期：2010-10-01 00:00:00

abstract：:Protein-A or goat anti-mouse-Ig (GAMIg) covalently bound to agarose-polyacrolein microsphere beads (APAMB) were employed for the removal of T cells from human peripheral blood leukocytes (PBL) and bone marrow (BM). The cell suspensions were treated with a monoclonal anti-T cell antibody (Leu-1) or monoclonal antilymph...

journal_title：Applied biochemistry and biotechnology

authors： Weiss L,Margel S,Slavin S

更新日期：1986-10-01 00:00:00

abstract：:The enzymatic hydrolysates of the lipid-extracted microalgal biomass residues (LMBRs) from biodiesel production were evaluated as nutritional sources for the mixotrophic growth of Chlorella vulgaris and lipid production at different temperature levels and substrate concentrations. Both parameters had a significant eff...

journal_title：Applied biochemistry and biotechnology

authors： Ma X,Zheng H,Huang H,Liu Y,Ruan R

更新日期：2014-10-01 00:00:00

abstract：:The increase of multidrug-resistant pathogens and the restriction on the use antibiotics due to its side effects have drawn attention to the search for possible alternatives. Bacteriocins are small antimicrobial peptides produced by numerous bacteria. Much interest has been focused on bacteriocins because they exhibit...

journal_title：Applied biochemistry and biotechnology

authors： Jena PK,Trivedi D,Chaudhary H,Sahoo TK,Seshadri S

更新日期：2013-04-01 00:00:00

abstract：:Biosorption of metal ions with Penicillium chrysogenum mycelium is described in this article. Alkaline pretreatment was used to remove proteins and nucleic acids from cells, and this treatment increased the adsorption capacities, for Cr3+ from 18.6 mg g(-1) to 27.2 mg g(-1), for Ni2+ from 13.2 mg g(-1) to 19.2 mg g(-1...

journal_title：Applied biochemistry and biotechnology

authors： Tan T,Cheng P

更新日期：2003-02-01 00:00:00

abstract：:A new process for enzymatic synthesis of biodiesel at high water content (10-20%) with 96% conversion by lipase from Candida sp. 99-125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized lipase could be used at least six times with high conve...

journal_title：Applied biochemistry and biotechnology

authors： Tan T,Nie K,Wang F

更新日期：2006-02-01 00:00:00

abstract：:The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

journal_title：Applied biochemistry and biotechnology

authors： Sousa CA,Hanselaer S,Soares EV

更新日期：2015-01-01 00:00:00

abstract：:The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran P,Narayanan GK,Gandhi S,Sethuraman S,Krishnan UM

更新日期：2015-03-01 00:00:00

abstract：:Batch fermentations of sugar cane bagasse hemicellulosic hydrolysate treated for removing the inhibitors of the fermentation were performed by Candida guilliermondii FTI20037 for xylitol production. The fermentative parameters agitation and aeration rate were studied aiming the maximization of xylitol production from ...

journal_title：Applied biochemistry and biotechnology

authors： Silva SS,Ribeiro JD,Felipe MG,Vitolo M

更新日期：1997-04-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:In this work, fibronectin purification from human plasma with the gelatin-immobilised poly(hydroxyethyl methacrylate) (PHEMA) cryogel has been evaluated. The PHEMA cryogel was prepared by cryo-polymerisation which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. The PHEMA cryogel contained i...

journal_title：Applied biochemistry and biotechnology

authors： Perçin I,Aksöz E,Denizli A

更新日期：2013-09-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:Paecilomyces variotii isolated from a broad range of habitats drives the diversification of new high-value-added secondary metabolites that could potentially play an important role in human and animal health. These metabolites include the anhydride metabolite of the nonadride family, as well as the following compounds...

journal_title：Applied biochemistry and biotechnology

authors： Mioso R,Toledo Marante FJ,Herrera Bravo de Laguna I

更新日期：2015-10-01 00:00:00

abstract：:Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on sub...

journal_title：Applied biochemistry and biotechnology

authors： Rao K,Chodisetti B,Gandi S,Giri A,Kishor PB

更新日期：2016-10-01 00:00:00

abstract：:Response surface methodology was employed to optimize culture medium for production of lipase with Rhodotorula sp. MTCC 8737. In the first step, a Plackett-Burman design was used to evaluate the effects of different inducers qualitatively. Of all the seven inducers tested, soybean oil showed significant influence on t...

journal_title：Applied biochemistry and biotechnology

authors： Chennupati S,Potumarthi R,Gopal Rao M,Manga PL,Sridevi M,Jetty A

更新日期：2009-11-01 00:00:00

abstract：:Current estimates indicate that hepatocarcinoma is the leading cause of death globally. There is interest in utilizing nanomedicine for cancer therapy to overcome side effects of chemo-interventions. Ribavirin, an antiviral nucleoside inhibitor, accumulates inside red blood cells, causing anemia. Its analog, viramidin...

journal_title：Applied biochemistry and biotechnology

authors： Abd-Rabou AA,Bharali DJ,Mousa SA

更新日期：2020-01-01 00:00:00

abstract：:A full factorial design leading to 20 sets of sorption runs was conducted to study the influence of four variables (bleaching earth and biomass concentrations, pH, and sorption time) on the iron, nickel, and chromium removal from stainless steel effluent using waste biomass from a beverage industry. Similar factor eff...

journal_title：Applied biochemistry and biotechnology

authors： Dias MA,Rosa CA,Linardi VR,Conte RA,De Castro HF

更新日期：2001-04-01 00:00:00

abstract：:This study investigated methane production in an anaerobic sequencing batch biofilm reactor (AnSBBR) by co-digesting sugarcane vinasse and cheese whey. The assessment was based on the influence of feed strategy, interaction between cycle time and influent concentration, applied volumetric organic load (OLRA), and temp...

journal_title：Applied biochemistry and biotechnology

authors： Sousa SP,Lovato G,Albanez R,Ratusznei SM,Rodrigues JAD

更新日期：2019-11-01 00:00:00

abstract：:The potential market for lactic acid as the feedstock for biodegradable polymers, oxygenated chemicals, and specialty chemicals is significant. L-lactic acid is often the desired enantiomer for such applications. However, stereospecific lactobacilli do not metabolize starch efficiently. In this work, Argonne researche...

journal_title：Applied biochemistry and biotechnology

authors： Tsai SP,Moon SH

更新日期：1998-04-01 00:00:00

abstract：:Electrokinetic-microbial remediation (EMR) has emerged as a promising option for the removal of polycyclic aromatic hydrocarbons (PAHs) from contaminated soils. The aim of this study was to enhance degradation of phenanthrene (Phe)-contaminated soils using EMR combined with biosurfactants. The electrokinetic (EK) reme...

journal_title：Applied biochemistry and biotechnology

authors： Lin W,Guo C,Zhang H,Liang X,Wei Y,Lu G,Dang Z

更新日期：2016-04-01 00:00:00

abstract：:Foam fractionation is one of the low operating-cost techniques for removing proteins from a dilute solution. The initial bulk solution pH and air superficial velocity play an important role in the foam-fractionation process. Denaturation of proteins (enzymes) can occur, however, during the foam-fractionation process f...

journal_title：Applied biochemistry and biotechnology

authors： Loha V,Prokop A,Du L,Tanner RD

更新日期：1999-04-01 00:00:00

abstract：:The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...

journal_title：Applied biochemistry and biotechnology

authors： Selma VC,Cotrim LH,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-12-01 00:00:00

abstract：:The factors affecting decolorization of anthraquinone dye represented by Reactive Blue 4 (RB4) and azo dye represented by Methyl Orange (MO) were studied in batch experiments under mesophilic (35 degrees C) and thermophilic (55 degrees C) anaerobic conditions. The results indicated differences in decolorization proper...

journal_title：Applied biochemistry and biotechnology

authors： Boonyakamol A,Imai T,Chairattanamanokorn P,Higuchi T,Sekine M

更新日期：2009-07-01 00:00:00