Maximizing the xylitol production from sugar cane bagasse hydrolysate by controlling the aeration rate.

abstract：:For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expressi...

journal_title：Applied biochemistry and biotechnology

authors： Xiao B,Li Q,Feng B,Han Z,Gao D,Zhao R,Li J,Li K,Zhi X,Yang H,Liu Z

更新日期：2009-06-01 00:00:00

abstract：:The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electroc...

journal_title：Applied biochemistry and biotechnology

authors： Singh S,Kaushal A,Khare S,Kumar A

更新日期：2014-05-01 00:00:00

abstract：:The increasing death rates related to anaplastic lymphoma kinase (ALK)-positive lung cancer culminated in a significant interest in the discovery of novel inhibitors for ALK. In the present research work, pharmacophore-based 3D QSAR modeling and virtual screening strategy have been carried out to address these issues....

journal_title：Applied biochemistry and biotechnology

authors： James N,Shanthi V,Ramanathan K

更新日期：2018-05-01 00:00:00

abstract：:An economic evaluation model was developed in the Laboratory of Agricultural Structures (LAS) of the Agricultural University of Athens-the Modified Basic Economic Evaluation Model (MBEEM). This model is an improved version of the original Basic Economic Evaluation Model, available in LAS, and it is used to assess the ...

journal_title：Applied biochemistry and biotechnology

authors： Georgakakis D,Christopoulou N,Chatziathanassiou A,Venetis T

更新日期：2003-04-01 00:00:00

abstract：:Artemisinin is an important drug commonly used in the treatment of malaria as a combination therapy. It is primarily produced by a plant Artemisia annua, however, its supply from plant is significantly lower than its huge demand and therefore alternative in vitro production routes are sought. Hairy root cultivation co...

journal_title：Applied biochemistry and biotechnology

authors： Patra N,Srivastava AK

更新日期：2014-11-01 00:00:00

abstract：:To develop more potent thrombolytic agents with fibrinolytic and antiplatelet aggregation activity, staphylokinase (Sak) variant Y1-Sak, a recombinant mutant of the Staphylococcus aureus protein Sak, was constructed. Y1-Sak formed an insoluble inclusion body when overexpressed in Escherichia coli strain JF1125. To obt...

journal_title：Applied biochemistry and biotechnology

authors： He J,Wang G,Xu R,Feng J,Wang J,Su H,Song H

更新日期：2008-10-01 00:00:00

abstract：:A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptase...

journal_title：Applied biochemistry and biotechnology

authors： Omidinia E,Shadjou N,Hasanzadeh M

更新日期：2014-02-01 00:00:00

abstract：:In this work, the catalytic conversion of natural polymer chitosan to 5-HMF under low temperature hydrothermal condition was investigated. The highest 5-HMF yield of 12.1 wt% was obtained under the following conditions: 174 °C reaction temperature, 2.2 % catalyst amount, and 36.9 min reaction time. In the effect of co...

journal_title：Applied biochemistry and biotechnology

authors： Lee SB,Jeong GT

更新日期：2015-06-01 00:00:00

abstract：:Lignocellulosic biomass has great potential as a cheap feedstock in biological processes to produce biofuels or chemicals; however, dilute acid pretreatment at high temperatures produces undesirable compounds. Toxicity tests were done with inhibitors in standard media, to predict the growth-limiting effects on thermop...

journal_title：Applied biochemistry and biotechnology

authors： Thomasser C,Danner H,Neureiter M,Saidi B,Braun R

更新日期：2002-04-01 00:00:00

abstract：:Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Al...

journal_title：Applied biochemistry and biotechnology

authors： Xiong J,Cao SL,Zong MH,Lou WY,Wu XL

更新日期：2020-10-01 00:00:00

abstract：:Prot-2 protease previously purified to homogeneity from Botrytis cinerea showed potentiality to be used in detergency and for production of bioactive peptides. To extend the characterization of Prot-2 protease, antifungal and antibacterial assays were performed in vitro using protein hydrolysates prepared from muscle ...

journal_title：Applied biochemistry and biotechnology

authors： Abidi F,Aissaoui N,Gaudin JC,Chobert JM,Haertlé T,Marzouki MN

更新日期：2013-05-01 00:00:00

abstract：:Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...

journal_title：Applied biochemistry and biotechnology

authors： Grunfeld H,Patel A,Shatzman A,Nishikawa AH

更新日期：1992-05-01 00:00:00

abstract：:Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...

journal_title：Applied biochemistry and biotechnology

authors： Kodrík D,Kludkiewicz B,Navrátil O,Skoková Habuštová O,Horáčková V,Svobodová Z,Vinokurov KS,Sehnal F

更新日期：2013-09-01 00:00:00

abstract：:Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

journal_title：Applied biochemistry and biotechnology

authors： Ximenes EA,Brandon SK,Doran-Peterson J

更新日期：2008-03-01 00:00:00

abstract：:Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to for...

journal_title：Applied biochemistry and biotechnology

authors： Bhuvana M,Dharuman V

更新日期：2014-10-01 00:00:00

abstract：:The enzymatic hydrolysates of the lipid-extracted microalgal biomass residues (LMBRs) from biodiesel production were evaluated as nutritional sources for the mixotrophic growth of Chlorella vulgaris and lipid production at different temperature levels and substrate concentrations. Both parameters had a significant eff...

journal_title：Applied biochemistry and biotechnology

authors： Ma X,Zheng H,Huang H,Liu Y,Ruan R

更新日期：2014-10-01 00:00:00

abstract：:Methanol, acetone and diethyl ether extracts of Alpinia galanga have been evaluated against pathogens viz. Bacillus subtilis MTCC 2391, Enterobacter aerogene, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli MTCC 1563, Klebsiella pneumoniae, Pseudomonas aeruginosa MTCC 6642, Salmonella typhimurium, Staphy...

journal_title：Applied biochemistry and biotechnology

authors： Rao K,Ch B,Narasu LM,Giri A

更新日期：2010-10-01 00:00:00

abstract：:Two protocols for functionalization of glass supports with hexaethylene glycol (HEG)-linked oligonucleotides were developed. The first method (standard amidite protocol) made use of the 2-cyanoethyl-phosphoramidite derivative of 4,4'-dimethoxytrityl-protected HEG. This was first coupled to the support by standard soli...

journal_title：Applied biochemistry and biotechnology

authors： Sojka B,Piunno PAE,Wust CC,Krull UJ

更新日期：2000-10-01 00:00:00

abstract：:Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

journal_title：Applied biochemistry and biotechnology

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2005-03-01 00:00:00

abstract：:This study presents the production of biodiesel from algae oil by transesterification using thermophilic microorganism. The microorganism used in this study was isolated from the soil sample obtained near the furnace. The organism was identified as Bacillus sp., and the lipase obtained was purified by ammonium sulfate...

journal_title：Applied biochemistry and biotechnology

authors： Sivaramakrishnan R,Muthukumar K

更新日期：2012-02-01 00:00:00

abstract：:The importance of using tissue-specific promoters in the genetic transformation of plants has been emphasized increasingly. Here, we report the isolation of a novel seed-specific promoter region from peanut and its validation in Arabidopsis and tobacco seeds. The reported promoter region referred to as groundnut seed ...

journal_title：Applied biochemistry and biotechnology

authors： Sunkara S,Bhatnagar-Mathur P,Sharma KK

更新日期：2014-01-01 00:00:00

abstract：:Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnost...

journal_title：Applied biochemistry and biotechnology

authors： Wai-Hoe L,Wing-Seng L,Ismail Z,Lay-Harn G

更新日期：2009-10-01 00:00:00

abstract：:The amount of corn stover and wheat straw that can be sustainably collected in North Carolina was estimated to be 0.64 and 0.16 million dry t/yr, respectively. More than 80% of these crop residues are located in the coastal area. The bioethanol potential from corn stover and wheat straw was estimated to be about 238 m...

journal_title：Applied biochemistry and biotechnology

authors： Shahbazi A,Li Y

更新日期：2006-04-01 00:00:00

abstract：:γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC 4.1.1.15) is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...

journal_title：Applied biochemistry and biotechnology

authors： Xiong Q,Xu Z,Xu L,Yao Z,Li S,Xu H

更新日期：2017-12-01 00:00:00

abstract：:Lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. To provide chimeric Bacillus thermocatenulatus lipase (BTL2), the completely conserved pentapeptide (¹¹²Ala-His-Ser-Gln-Gly¹¹⁶) was replaced with similar sequences (²⁰⁷Gly-G...

journal_title：Applied biochemistry and biotechnology

authors： Hosseini M,Karkhane AA,Yakhchali B,Shamsara M,Aminzadeh S,Morshedi D,Haghbeen K,Torktaz I,Karimi E,Safari Z

更新日期：2013-02-01 00:00:00

abstract：:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

journal_title：Applied biochemistry and biotechnology

authors： Nakanishi K,Ikebukuro K,Karube I

更新日期：1996-08-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensa...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Yao M,Fang X,Yang Y,Cheng X

更新日期：2015-11-01 00:00:00

abstract：:We applied a ribosome display technique to a mouse single chain variable fragment (scFv) library to select scFvs specific for the inducible costimulator (ICOS). mRNA was isolated from the spleens of BALB/c mice immunized with ICOS protein. Heavy and κ chain genes (VH and κ) were amplified separately by reverse transcr...

journal_title：Applied biochemistry and biotechnology

authors： Pan Y,Mao W,Liu X,Xu C,He Z,Wang W,Yan H

更新日期：2012-11-01 00:00:00

abstract：:An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish peroxidase, 4-amino-phenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis, with free and immobilized enzymes. The system with free enzymes pe...

journal_title：Applied biochemistry and biotechnology

authors： Alhadeff EM,Salgado AM,Pereira N Jr,Valdman B

更新日期：2004-04-01 00:00:00