Isolation and functional characterization of a novel seed-specific promoter region from peanut.

Abstract:

:The importance of using tissue-specific promoters in the genetic transformation of plants has been emphasized increasingly. Here, we report the isolation of a novel seed-specific promoter region from peanut and its validation in Arabidopsis and tobacco seeds. The reported promoter region referred to as groundnut seed promoter (GSP) confers seed-specific expression in heterologous systems, which include putative promoter regions of the peanut (Arachis hypogaea L.) gene 8A4R19G1. This region was isolated, sequenced, and characterized using gel shift assays. Tobacco transgenics obtained using binary vectors carrying uidA reporter gene driven by GSP and/or cauliflower mosaic virus 35S promoters were confirmed through polymerase chain reaction (PCR), RT-PCR, and computational analysis of motifs which revealed the presence of TATA, CAAT boxes, and ATG signals. This seed-specific promoter region successfully targeted the reporter uidA gene to seed tissues in both Arabidopsis and tobacco model systems, where its expression was confirmed by histochemical analysis of the transgenic seeds. This promoter region is routinely being used in the genetic engineering studies in legumes aimed at targeting novel transgenes to the seeds, especially those involved in micronutrient enhancement, fungal resistance, and molecular pharming.

authors

Sunkara S,Bhatnagar-Mathur P,Sharma KK

doi

10.1007/s12010-013-0482-x

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

325-39

issue

1

eissn

0273-2289

issn

1559-0291

journal_volume

172

pub_type

杂志文章
  • Enhanced Enzymatic Hydrolysis of Sorghum Stalk by Supercritical Carbon Dioxide and Ultrasonic Pretreatment.

    abstract::Sorghum was pretreated by sole ultrasound or supercritical carbon dioxide (scCO2), as well as the method combining both to intensify enzymatic hydrolysis. The effect of the time (1-5 h) and temperature (30-70 °C) on ultrasonic pretreatment was investigated, and the best condition was determined as 5 h and 50 °C with t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2909-x

    authors: Zhang Q,Zhao M,Xu Q,Ren H,Yin J

    更新日期:2019-05-01 00:00:00

  • Temperature-dependent behavior of immobilized alkaline phosphatase. I. Role of working conditions and pH during coupling.

    abstract::Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798297

    authors: Pittner F

    更新日期:1982-05-01 00:00:00

  • Preferential lectin binding of cancer cells upon sialic acid treatment under nutrient deprivation.

    abstract::The terminal monosaccharide of glycoconjugates on a eukaryotic cell surface is typically a sialic acid (Neu5Ac). Increased sialylation usually indicates progression and poor prognosis of most carcinomas. Here, we utilize two human mammary epithelial cell lines, HB4A (breast normal cells) and T47D (breast cancer cells)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0409-6

    authors: Badr HA,Elsayed AI,Ahmed H,Dwek MV,Li CZ,Djansugurova LB

    更新日期:2013-10-01 00:00:00

  • Maximum production of fermentable sugars from barley straw using optimized soaking in aqueous ammonia (SAA) pretreatment.

    abstract::Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0154-x

    authors: Yoo CG,Nghiem NP,Hicks KB,Kim TH

    更新日期:2013-04-01 00:00:00

  • Crosslinked penicillin acylase aggregates for synthesis of beta-lactam antibiotics in organic medium.

    abstract::Crosslinked enzyme aggregates (CLEAs) of a partially purified penicillin acylase from a recombinant Escherichia coli strain have been produced as a novel type of biocatalysts well endowed to perform in organic media. Different protein precipitants were studied and glutaraldehyde was used as the crosslinking agent. Pre...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:133:3:189

    authors: Illanes A,Wilson L,Caballero E,Fernández-Lafuente R,Guisán JM

    更新日期:2006-06-01 00:00:00

  • Ex vitro expansion of human placenta-derived mesenchymal stem cells in stirred bioreactor.

    abstract::The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs proliferation has yet been reported. In the present study, the feas...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8556-5

    authors: Yu Y,Li K,Bao C,Liu T,Jin Y,Ren H,Yun W

    更新日期:2009-10-01 00:00:00

  • Patterns of Lignocellulosic Sugar Assimilation and Lipid Production by Newly Isolated Yeast Strains From Chilean Valdivian Forest.

    abstract::Three yeast strains were isolated from decaying wood of Chilean Valdivian forest and identified as Meyerozyma guilliermondii, Scheffersomyces coipomensis, and Sugiyamaella paludigena. These strains were able to efficiently grow on the major monomers contained in Pinus spp. and Eucalyptus spp. wood that includes glucos...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03398-4

    authors: Valdés G,Mendonça RT,Parra C,Aggelis G

    更新日期:2020-12-01 00:00:00

  • Purification and partial characterization of bacillomycin L produced by Bacillus amyloliquefaciens K103 from lemon.

    abstract::Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0424-7

    authors: Zhang B,Dong C,Shang Q,Cong Y,Kong W,Li P

    更新日期:2013-12-01 00:00:00

  • Process development for production of medium chain triglycerides using immobilized lipase in a solvent-free system.

    abstract::The synthesis of tricaprylin, tricaprin, trilaurin, and trimyristin in a solvent-free system was conducted by mixing a commercial immobilized lipase with the organic reagents (glycerol and fatty acid) in a 20-mL batch reactor with constant stirring. The effects of temperature, fatty acid/glycerol molar ratio, and enzy...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:98-100:1-9:997

    authors: Langone MA,Sant'Anna GL Jr

    更新日期:2002-04-01 00:00:00

  • Purification and partial characterization of lignin peroxidase from Acinetobacter calcoaceticus NCIM 2890 and its application in decolorization of textile dyes.

    abstract::Lignin peroxidase was purified (72-fold) from Acinetobacter calcoaceticus NCIM 2890. The purified lignin peroxidase (55-65 kDa) showed dimeric nature. The maximum enzyme activity was observed at pH 1.0, between a broad temperature range of 50 and 70 degrees C, at H2O2 concentration (40 mM) and the substrate concentrat...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8258-4

    authors: Ghodake GS,Kalme SD,Jadhav JP,Govindwar SP

    更新日期:2009-01-01 00:00:00

  • Evaluation of recombinant green fluorescent protein, under various culture conditions and purification with HiTrap hydrophobic interaction chromatography resins.

    abstract::To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:114:1-3:453

    authors: Penna TC,Ishii M,Junior AP,de Oliveira Nascimento L,de Souza LC,Cholewa O

    更新日期:2004-04-01 00:00:00

  • Co-expression of recombinant nucleoside phosphorylase from Escherichia coli and its application.

    abstract::The genes encoding purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase), and thymidine phosphorylase (TPase) from Escherichia coli K12 were cloned respectively into expression vector pET-11a or pET-28a. The recombinant plasmids were transformed into the host strain E. coli BL21(DE3) to construct fou...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8429-3

    authors: Ge C,OuYang L,Ding Q,Ou L

    更新日期:2009-10-01 00:00:00

  • Potentiometric response of lipid modified ISFET.

    abstract::The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02918526

    authors: Osa T

    更新日期:1993-04-01 00:00:00

  • Carbon Neutral Electricity Production from Municipal Solid Waste Landfill Leachate Using Algal-Assisted Microbial Fuel Cell.

    abstract::We demonstrate the feasibility of algal lipid production and bioelectricity generation with concurrent treatment of municipal solid waste (MSW) leachate in a double-chamber algal-assisted microbial fuel cell (AAMFC). The cathode chamber was loaded with Synechococcus sp. and MSW leachate whereas anode chamber was loade...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03160-5

    authors: Lakshmidevi R,Gandhi NN,Muthukumar K

    更新日期:2020-06-01 00:00:00

  • Molecular modification of Protein A to improve the elution pH and alkali resistance in affinity chromatography.

    abstract::Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0818-1

    authors: Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

    更新日期:2014-04-01 00:00:00

  • Ultrafiltration of thin stillage from conventional and e-mill dry grind processes.

    abstract::We used ultrafiltration (UF) to evaluate membrane filtration characteristics of thin stillage and determine solids and nutrient compositions of filtered streams. To obtain thin stillage, corn was fermented using laboratory methods. UF experiments were conducted in batch mode under constant temperature and flow rate co...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9114-x

    authors: Arora A,Dien BS,Belyea RL,Wang P,Singh V,Tumbleson ME,Rausch KD

    更新日期:2011-05-01 00:00:00

  • Lactase production by solid-state cultivation of Kluyveromyces marxianus CDBBL 278 on an inert support: effect of inoculum, buffer, and nitrogen source.

    abstract::A study was carried out to select the conditions for cultivation of Kluyveromyces marxianus CDBBL 278 in solid-state culture (SSC) using polyurethane foam (PUF) as an inert support. PUF was impregnated with culture media containing lactose (50 g/L) as the carbon and energy source. Evaluation of culture parameters duri...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8268-2

    authors: Tovar-Castro L,García-Garibay M,Saucedo-Castañeda G

    更新日期:2008-12-01 00:00:00

  • A thermostable alkaline lipase from a local isolate Bacillus subtilis EH 37: characterization, partial purification, and application in organic synthesis.

    abstract::A mesophilic bacterial culture producing a novel thermostable alkaline lipase was isolated from oil rich soil sample and identified as Bacillus subtilis EH 37. The lipase was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography with 17.8-fold purification and 41.9 U/ml specif...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8751-4

    authors: Ahmed EH,Raghavendra T,Madamwar D

    更新日期:2010-04-01 00:00:00

  • Characterization and performance of immobilized amylase and cellulase.

    abstract::The performance of cellulase and amylase immobilized on siliceous supports was investigated. Enzyme uptake onto the support depended on the enzyme source and immobilization conditions. For amylase, the uptake ranged between 20 and 60%, and for cellulase, 7-10%. Immobilized amylase performance was assessed by batch kin...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:113:1-3:251

    authors: Saville BA,Khavkine M,Seetharam G,Marandi B,Zuo YL

    更新日期:2004-04-01 00:00:00

  • Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase.

    abstract::Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the format...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8557-4

    authors: Sørensen HP,Madsen LS,Petersen J,Andersen JT,Hansen AM,Beck HC

    更新日期:2010-03-01 00:00:00

  • An oxidant- and solvent-stable protease produced by Bacillus cereus SV1: application in the deproteinization of shrimp wastes and as a laundry detergent additive.

    abstract::The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obta...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8703-z

    authors: Manni L,Jellouli K,Ghorbel-Bellaaj O,Agrebi R,Haddar A,Sellami-Kamoun A,Nasri M

    更新日期:2010-04-01 00:00:00

  • Characteristics of Raw Starch-Digesting α-Amylase of Streptomyces badius DB-1 with Transglycosylation Activity and Its Applications.

    abstract::Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2284-4

    authors: Shivlata L,Satyanarayana T

    更新日期:2017-04-01 00:00:00

  • Importance of C-Terminal Extension in Thermophilic 1,4-α-Glucan Branching Enzyme from Geobacillus thermoglucosidans STB02.

    abstract::By sequence comparison, the majority of 1,4-α-glucan-branching enzymes (GBEs) consist of an N-terminal carbohydrate-binding domain, a TIM-barrel catalytic domain, and a C-terminal all-beta domain. Among these structures, the GBE from Geobacillus thermoglucosidans STB02 uniquely has a highly charged 26-amino-acid C-ter...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03150-7

    authors: Ban X,Li C,Zhang Y,Gu Z,Cheng L,Hong Y,Li Z

    更新日期:2020-03-01 00:00:00

  • Cloning, sequence, and association analysis of porcine OSAP gene.

    abstract::Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9788-3

    authors: Lei Y,Yonggang L

    更新日期:2012-09-01 00:00:00

  • Modification of lysine residues of horseradish peroxidase and its effect on stability and structure of the enzyme.

    abstract::Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0756-y

    authors: Hassani L,Nourozi R

    更新日期:2014-04-01 00:00:00

  • Construction of a genetically engineered microorganism that simultaneously degrades organochlorine and organophosphate pesticides.

    abstract::Field contamination with pesticide mixtures of organophosphates (OPs) and organochlorines (OCs) is becoming global issues to be solved urgently. The strategy of utilizing engineered microorganisms that have an ability to simultaneously degrade OPs and OCs has increasingly received great interest. In this work, an OP d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9450-5

    authors: Yang J,Liu R,Song W,Yang Y,Cui F,Qiao C

    更新日期:2012-02-01 00:00:00

  • Prevalence of genetically modified rice, maize, and soy in Saudi food products.

    abstract::Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified org...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0405-x

    authors: Elsanhoty RM,Al-Turki AI,Ramadan MF

    更新日期:2013-10-01 00:00:00

  • Regeneration of nicotinamide cofactors for use in organic synthesis.

    abstract::The high cost of nicotinamide cofactors requires that they be regenerated in situ when used in preparative enzymatic synthesis. Numerous strategies have been tested for in situ regeneration of reduced and oxidized cofactors. Regeneration of reduced cofactors is relatively straightforward; regeneration of oxidized cofa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF02798431

    authors: Chenault HK,Whitesides GM

    更新日期:1987-03-01 00:00:00

  • Bovine Lactoferrampin, Human Lactoferricin, and Lactoferrin 1-11 Inhibit Nuclear Translocation of HIV Integrase.

    abstract::This study aimed to investigate fragments derived from human and bovine lactoferrins for ability to inhibit nuclear translocation of HIV-1 integrase. It was shown that human lactoferricin, human lactoferrin 1-11, and bovine lactoferrampin reduced nuclear distribution of HIV-1 integrase. Bovine lactoferrampin could inh...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2059-y

    authors: Wang WY,Wong JH,Ip DT,Wan DC,Cheung RC,Ng TB

    更新日期:2016-08-01 00:00:00

  • pH-Based Detection of Target Analytes in Diluted Serum Samples Using Surface Plasmon Resonance Immunosensor.

    abstract::Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2883-3

    authors: Rath D,Kumar S,Panda S

    更新日期:2019-04-01 00:00:00