Expression of recombinant human nerve growth factor beta in milk of goats by recombinant replication-defective adenovirus.

Abstract:

:For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expression level of recombinant hNGF-beta in the milk reached 196.8 mg/l. The biological activity of the recombinant hNGF-beta, compared with the commercial hNGF-beta, was tested using PC12 cells. The results demonstrate that instilling recombinant adenovirus directly into the mammary gland of goat is an efficient approach to producing a large quantity of hNGF-beta.

authors

Xiao B,Li Q,Feng B,Han Z,Gao D,Zhao R,Li J,Li K,Zhi X,Yang H,Liu Z

doi

10.1007/s12010-008-8346-5

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

357-66

issue

3

eissn

0273-2289

issn

1559-0291

journal_volume

157

pub_type

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