Abstract:
:For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expression level of recombinant hNGF-beta in the milk reached 196.8 mg/l. The biological activity of the recombinant hNGF-beta, compared with the commercial hNGF-beta, was tested using PC12 cells. The results demonstrate that instilling recombinant adenovirus directly into the mammary gland of goat is an efficient approach to producing a large quantity of hNGF-beta.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Xiao B,Li Q,Feng B,Han Z,Gao D,Zhao R,Li J,Li K,Zhi X,Yang H,Liu Zdoi
10.1007/s12010-008-8346-5subject
Has Abstractpub_date
2009-06-01 00:00:00pages
357-66issue
3eissn
0273-2289issn
1559-0291journal_volume
157pub_type
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