当前位置： SCI文献检索 > APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY期刊下所有文献 > Purification and characterization of paraoxonase 1 (PON1) from Swiss Black, Holstein, and Montofon bovines.

Purification and characterization of paraoxonase 1 (PON1) from Swiss Black, Holstein, and Montofon bovines.

Abstract：

:Paraoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals and other vertebrates as well as in invertebrates. PONs exhibit a range of important activities, including drug metabolism and detoxification of organophosphates such as nerve agents. This study reports, for the first time, purification and biochemical characterization of serum PON1 from different bovine breeds namely Swiss Black, Holstein, and Montofon. Bovine serum PON1s were purified using ammonium sulfate precipitation followed by Sepharose-4B-L-tyrosine-1-naphthylamine hydrophobic interaction chromatography. SDS-polyacrylamide gel electrophoresis of the purified enzymes indicates a single band with an apparent MW of 43 kDa. The purified enzymes had a specific activity of 10.78, 27.00, and 22.38 U/mg for Swiss Black, Holstein, and Montofon bovines, respectively. The overall purification rates of our method were 262.47-, 2,476.90-, and 538.06-fold for Swiss Black, Holstein, and Montofon bovines, respectively. Furthermore, using phenyl acetate as a substrate, we determined the K M and V max values of the purified enzymes, as 0.80 mM, 1428.5 U/ml for Swiss Black; 0.40 mM, 714.3 U/ml for Holstein; and 0.50 mM, 1,111.1 U/ml for Montofon bovine. The present study has revealed that there is no substantial difference in PON1 activities among the studied bovine breeds.

journal_name

Appl Biochem Biotechnol

journal_title

Applied biochemistry and biotechnology

authors

Erzengin M,Demir D,Arslan M,Sinan S

doi

10.1007/s12010-014-0931-1

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

1597-606

issue

7

eissn

0273-2289

issn

1559-0291

journal_volume

173

pub_type

杂志文章

相关文献

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY文献大全
  • Mushroom polysaccharides and lipids synthesized in liquid agitated and static cultures. Part I: screening various mushroom species.

    abstract：:The effect of four synthetic media containing glucose (initial concentration 30 g l(-1)) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also ...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-012-9713-9

    authors： Diamantopoulou P,Papanikolaou S,Kapoti M,Komaitis M,Aggelis G,Philippoussis A

    更新日期：2012-06-01 00:00:00

  • A novel beta-mannanase with high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and enzymatic characterization.

    abstract：:A DNA fragment of 2,042 bp containing a novel beta-mannanase gene, man5A, was identified from the genome of the mannan-degrading bacterium Bacillus circulans CGMCC1554. The open reading frame of man5A comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kDa. The amino acid seq...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-008-8364-3

    authors： Yang P,Li Y,Wang Y,Meng K,Luo H,Yuan T,Bai Y,Zhan Z,Yao B

    更新日期：2009-10-01 00:00:00

  • A trypsin inhibitor from rambutan seeds with antitumor, anti-HIV-1 reverse transcriptase, and nitric oxide-inducing properties.

    abstract：:Nephelium lappaceum L., commonly known as "rambutan," is a typical tropical tree and is well known for its juicy and sweet fruit which has an exotic flavor. Chemical studies on rambutan have led to the identification of various components such as monoterpene lactones and volatile compounds. Here, a 22.5-kDa trypsin in...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-015-1550-1

    authors： Fang EF,Ng TB

    更新日期：2015-04-01 00:00:00

  • In silico characterization of alkaline proteases from different species of Aspergillus.

    abstract：:A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. The sequence level h...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-011-9420-y

    authors： Morya VK,Yadav S,Kim EK,Yadav D

    更新日期：2012-01-01 00:00:00

  • Production and characteristics of the whole-cell lipase from organic solvent tolerant Burkholderia sp. ZYB002.

    abstract：:The thermostable and organic solvent tolerant whole-cell lipase (WCL) was produced by Burkholderia sp. ZYB002 with broad spectrum organic solvent tolerance. The production medium of the WCL was primarily optimized, which resulted in the maximum activity of 22.8 U/mL and the 5.1-fold increase of the WCL yield. The opti...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-011-9446-1

    authors： Shu ZY,Wu JG,Cheng LX,Chen D,Jiang YM,Li X,Huang JZ

    更新日期：2012-02-01 00:00:00

  • Characteristics of oxidation-reduction potential, VFAs, SCOD, N, and P in an ATAD system under different thermophilic temperatures.

    abstract：:One-stage autoheated thermophilic aerobic digestion (ATAD) can stabilize sludge to meet class A standard. In this study, batch experiments were conducted to investigate the characteristics of oxidation-reduction potential (ORP), volatile fatty acids (VFAs), soluble chemical oxygen demand (SCOD), and nutrients at diffe...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-014-1241-3

    authors： Cheng J,Kong F,Zhu J,Wu X

    更新日期：2015-01-01 00:00:00

  • Detergent-compatible bacterial amylases.

    abstract：:Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of usi...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章,评审

    doi：10.1007/s12010-014-1144-3

    authors： Niyonzima FN,More SS

    更新日期：2014-10-01 00:00:00

  • Induction of a high-yield lovastatin mutant of Aspergillus terreus by ¹²C⁶⁺ heavy-ion beam irradiation and the influence of culture conditions on lovastatin production under submerged fermentation.

    abstract：:Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-011-9308-x

    authors： Li SW,Li M,Song HP,Feng JL,Tai XS

    更新日期：2011-10-01 00:00:00

  • Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

    abstract：:An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination ...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-015-1732-x

    authors： Fatima N,Ahmad N,Ahmad I,Anis M

    更新日期：2015-09-01 00:00:00

  • High-yield hypocrellin A production in solid-state fermentation by Shiraia sp. SUPER-H168.

    abstract：:Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-009-8728-3

    authors： Cai Y,Liang X,Liao X,Ding Y,Sun J,Li X

    更新日期：2010-04-01 00:00:00

  • An Innovator Support Material for Tyrosinase Immobilization: Antimony-Doped Tin Oxide Thin Films (ATO-TF).

    abstract：:Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectrosc...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-020-03337-3

    authors： Türkhan A,Kaya ED,Koçyiğit A

    更新日期：2020-10-01 00:00:00

  • Improvement of bioethanol productivity of immobilized Saccharomyces bayanus with using sodium alginate-graft-poly(N-vinyl-2-pyrrolidone) matrix.

    abstract：:In this study, immobilization conditions and bioethanol production characteristics of immobilized Saccharomyces bayanus were investigated into sodium alginate-graft-poly(N-vinyl-2-pyrrolidone; NaAlg-g-PVP) matrix. The matrix that crosslinked with calcium clorid was used for immobilization of S. bayanus. Bioethanol pro...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-012-9770-0

    authors： İnal M,Yiğitoğlu M

    更新日期：2012-09-01 00:00:00

  • Comparison of catalytic properties of free and immobilized cellobiase novozym 188.

    abstract：:The enzyme cellobiase from Novo was immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67 and 13.7%, respectively. The activity of the free enzyme (FE) and immobilized enzyme (IE) was determined with 2 g/L of cellobiose, from 40...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1385/abab:91-93:1-9:615

    authors： Calsavara LP,De Moraes FF,Zanin GM

    更新日期：2001-04-01 00:00:00

  • Proteomics and detection of uromodulin in first-time renal calculi patients and recurrent renal calculi patients.

    abstract：:Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnost...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-008-8503-x

    authors： Wai-Hoe L,Wing-Seng L,Ismail Z,Lay-Harn G

    更新日期：2009-10-01 00:00:00

  • Enabling Heterologous Synthesis of Lupulones in the Yeast Saccharomyces cerevisiae.

    abstract：:Lupulones, naturally produced by glandular trichomes of hop (Humulus lupulus), are prenylated phloroglucinol derivatives that contribute the bitter flavor of beer and demonstrate antimicrobial and anticancer activities. It is appealing to develop microbial cell factories such that lupulones may be produced via ferment...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-019-02957-8

    authors： Guo X,Shen H,Liu Y,Wang Q,Wang X,Peng C,Liu W,Zhao ZK

    更新日期：2019-07-01 00:00:00

  • Effect of Carbon Sources on the Catalytic Performance of Ni/β-Mo2C.

    abstract：:In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs). The carburized catalyst samples were characterized by X-ray diffraction (XRD), sc...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-015-1622-2

    authors： Zeng LZ,Zhao SF,Li WS

    更新日期：2015-06-01 00:00:00

  • Saccharification of marine microalgae using marine bacteria for ethanol production.

    abstract：:The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydra...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1385/abab:105:1-3:247

    authors： Matsumoto M,Yokouchi H,Suzuki N,Ohata H,Matsunaga T

    更新日期：2003-04-01 00:00:00

  • Co-aggregation of laccase and nature egg white: a simple method to prepare stable and recyclable biocatalyst.

    abstract：:Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-013-0697-x

    authors： Jiang Y,Wang Q,He Y,Zhou L,Gao J

    更新日期：2014-03-01 00:00:00

  • Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase.

    abstract：:Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob. The immobilization is carried out by tosylation of primary alcohols of cellulose of corn cob. The immobilized benzonase is more stable vs pH changes than native benzonase, but the ...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF02922124

    authors： Moreno JM,Sanchez-Montero JM,Ballesteros A,Sinisterra JV

    更新日期：1991-10-01 00:00:00

  • 6-Tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues: synthesis, characterization, MO calculation, and antibacterial activity.

    abstract：:A series of 6-tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues are synthesized by conventional techniques and characterized by elemental analysis, IR, MS, (1)H, and (13)C NMR. These are tested for their antibacterial activity against Bacillus subtilis (abbreviated as BS), Staphylococcus aureus (ab...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-014-1399-8

    authors： Doshi H,Thakkar S,Khirsariya P,Thakur MC,Ray A

    更新日期：2015-02-01 00:00:00

  • Modulatory Effect of Indoles on the Expression of miRNAs Regulating G1/S Cell Cycle Phase in Breast Cancer Cells.

    abstract：:Indole-3-carbinol (I3C) is a naturally occurring glucosinolate found in Brassica vegetables that is usually converted in gastric acidic environment to the efficient metabolite 3,3'-diindolylmethane (DIM). Both indoles (I3C and DIM) are known chemopreventive agents for various cancers including breast cancer. This stud...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-020-03378-8

    authors： El-Daly SM,Gamal-Eldeen AM,Gouhar SA,Abo-Elfadl MT,El-Saeed G

    更新日期：2020-12-01 00:00:00

  • Optimizing carbon/nitrogen ratio for biosurfactant production by a Bacillus subtilis strain.

    abstract：:A Bacillus subtilis strain isolated from contaminated soil from a refinery has been screened for biosurfactant production in crystal sugar (sucrose) with different nitrogen sources (NaNO(3), (NH(4))(2)SO(4), urea, and residual brewery yeast). The highest reduction in surface tension was achieved with a 48-h fermentati...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-007-9073-z

    authors： Fonseca RR,Silva AJ,De França FP,Cardoso VL,Sérvulo EF

    更新日期：2007-04-01 00:00:00

  • Expression, purification, and antibacterial activity of bovine lactoferrampin-lactoferricin in Pichia pastoris.

    abstract：:Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N(1) domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical syn...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-011-9455-0

    authors： Tang XS,Tang ZR,Wang SP,Feng ZM,Zhou D,Li TJ,Yin YL

    更新日期：2012-02-01 00:00:00

  • A mathematical analysis of the selective enrichment of NECEEM-based non-SELEX.

    abstract：:Non-Systematic Evolution of Ligands by EXponential enrichment (SELEX)selection of aptamers, a novel technology for aptamer selection from libraries of random DNA (or RNA) sequences, involves repetitive steps of partitioning without polymerase chain reaction (PCR) amplification between them. This selection is based on ...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-014-0989-9

    authors： Yu X,Yu Y

    更新日期：2014-08-01 00:00:00

  • Performance testing of Zymomonas mobilis metabolically engineered for cofermentation of glucose, xylose, and arabinose.

    abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1385/abab:98-100:1-9:429

    authors： Lawford HG,Rousseau JD

    更新日期：2002-04-01 00:00:00

  • Polysaccharide hydrolase folds diversity of structure and convergence of function.

    abstract：:Polysaccharide glycosyl hydrolases are a group of enzymes that hydrolyze the glycosidic bond between carbohydrates or between a carbohydrate and a noncarbohydrate moiety. Here we illustrate that traditional schemes for grouping enzymes, such as by substrate specificity or by organism of origin, are not appropriate whe...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/BF02920433

    authors： Himmel ME,Karplus PA,Sakon J,Adney WS,Baker JO,Thomas SR

    更新日期：1997-04-01 00:00:00

  • Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures.

    abstract：:An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-009-8682-0

    authors： de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde L

    更新日期：2010-03-01 00:00:00

  • Use of corncob for endoxylanase production by thermophilic fungus Thermomyces lanuginosus IOC-4145.

    abstract：:The production of cellulase-free endoxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated in semisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid fermentation, employing corncob as the carbon source. The best results were with the following ...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1385/abab:84-86:1-9:821

    authors： Damaso MC,Andrade CM,Pereira Júnior N

    更新日期：2000-04-01 00:00:00

  • Construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production.

    abstract：:A novel expression system of Klebsiella pneumoniae was developed in order to improve 1,3-propanediol (1,3-PD) production using a K. pneumoniae-Escherichia coli shuttle vector pET28a consisting of the kanamycin-resistance gene promoter Pkan. The recombinant plasmid pETPkan-cat carrying the chloramphenicol acetyltransfe...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-009-8743-4

    authors： Ma Z,Rao Z,Zhuge B,Fang H,Liao X,Zhuge J

    更新日期：2010-09-01 00:00:00

  • Production of lipid and fatty acids during growth of Aspergillus terreus on hydrocarbon substrates.

    abstract：:An Aspergillus terreus, isolated from oil contaminated soil, could degrade a wide range of petroleum hydrocarbons including the immediate oxidation products of hydrocarbons, like alkanols and alkanals. Among all the linear chain carbon substrates, highest growth of 39.1 + or - 3.8 g l(-1) (wet weight) was observed whe...

    journal_title：Applied biochemistry and biotechnology

    pub_type： 杂志文章

    doi：10.1007/s12010-009-8669-x

    authors： Kumar AK,Vatsyayan P,Goswami P

    更新日期：2010-03-01 00:00:00