Saccharification of marine microalgae using marine bacteria for ethanol production.

abstract：:Enzymes from thermophilic microorganisms, thermozymes, have unique characteristics such as temperature, chemical, and pH stability. They can be used in several industrial processes, in which they replace mesophilic enzymes or chemicals. Thermozymes are often used when the enzymatic process is compatible with existing ...

journal_title：Applied biochemistry and biotechnology

authors： Bruins ME,Janssen AE,Boom RM

更新日期：2001-02-01 00:00:00

abstract：:A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

journal_title：Applied biochemistry and biotechnology

authors： Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

更新日期：2008-04-01 00:00:00

abstract：:The ethanologenic bacteria Escherichia coli strains KO11 and LYO1, and Klebsiella oxytoca strain P2, were investigated for their ability to metabolize furfural. Using high performance liquid chromatography and 13C-nuclear magnetic resonance spectroscopy, furfural was found to be completely biotransformed into furfuryl...

journal_title：Applied biochemistry and biotechnology

authors： Gutiérrez T,Buszko ML,Ingram LO,Preston JF

更新日期：2002-04-01 00:00:00

abstract：:L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The im...

journal_title：Applied biochemistry and biotechnology

authors： Kim KR,Seo ES,Oh DK

更新日期：2014-01-01 00:00:00

abstract：:Batch fermentations of sugar cane bagasse hemicellulosic hydrolysate treated for removing the inhibitors of the fermentation were performed by Candida guilliermondii FTI20037 for xylitol production. The fermentative parameters agitation and aeration rate were studied aiming the maximization of xylitol production from ...

journal_title：Applied biochemistry and biotechnology

authors： Silva SS,Ribeiro JD,Felipe MG,Vitolo M

更新日期：1997-04-01 00:00:00

abstract：:Seven white rot fungal species were tested for growth as mycelia using swine wastewater (SW), an agro-waste with tremendous environmental footprint, as the sole nutrient source. The SW contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. Out...

journal_title：Applied biochemistry and biotechnology

authors： Lee J,Shin SG,Ahn J,Han G,Hwang K,Kim W,Hwang S

更新日期：2017-02-01 00:00:00

abstract：:Extractive fermentation is a widely preferred technique in which the products of fermentation are removed from the fermentation medium by a proper solvent, in order to avoid the inhibitory effects of the products. In this work, decanol, which has a high distribution coefficient with respect to the biocompatible solven...

journal_title：Applied biochemistry and biotechnology

authors： Kapucu H,Mehmetoğlu U

更新日期：1998-11-01 00:00:00

abstract：:Two DNA fragments containing the entire coding sequences of lactate dehydrogenase (LDH; ldhL1 and ldhD), whose enzymes have high activity for bioconversion of phenylpyruvate (PPA) to phenyllactate (PLA), were amplified from Lactobacillus plantarum SK002 using PCR. Sequencing showed open reading frames of 963 bp (ldhL1...

journal_title：Applied biochemistry and biotechnology

authors： Jia J,Mu W,Zhang T,Jiang B

更新日期：2010-09-01 00:00:00

abstract：:A protein-engineered beta-lactamase, constructed by site-directed mutagenesis in Escherichia coli (E104M/G238S), and having broadened specificity, was able to degrade cephalosporins of first, second, and third generations. Manipulations of culture conditions allowed an increase in beta-lactamase specific activity by u...

journal_title：Applied biochemistry and biotechnology

authors： García JL,Nuñez CJ,González EG,Osuna J,Soberón X,Galindo E

更新日期：1998-05-01 00:00:00

abstract：:The autoimmune repertoire is well known from previous studies to be capable of producing catalytic antibodies directed to self-antigens. In the present study, we explored the ability of 26 monoclonal light chains (L chains) from multiple myeloma patients to cleave radiolabeled gp120, a foreign protein. One L chain wit...

journal_title：Applied biochemistry and biotechnology

authors： Paul S,Kalaga RS,Gololobov G,Brenneman D

更新日期：2000-01-01 00:00:00

abstract：:The use of cheap, high-efficiency, and ecofriendly adsorbent has been studied as an alternative way for the removal of dyes from wastewater. This paper investigated the use of waste brewery's yeast for the removal of acid orange 7 from aqueous solution. The optimum removal of acid orange 7 was found to be 3.561 mg/g a...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Hu Y,Xie Z,Feng S,Li B,Mi X

更新日期：2011-04-01 00:00:00

abstract：:Production costs of cellulosic biofuels can be lowered if cellulases are recovered and reused using particulate carriers that can be extracted after biomass hydrolysis. Such enzyme recovery was recently demonstrated using enzymogel nanoparticles with grafted polymer brushes loaded with cellulases. In this work, cellul...

journal_title：Applied biochemistry and biotechnology

authors： Samaratunga A,Kudina O,Nahar N,Zakharchenko A,Minko S,Voronov A,Pryor SW

更新日期：2015-06-01 00:00:00

abstract：:Residue of chili plants left in the field after harvesting is a major lignocellulosic resource that is underexploited. India has over 0.6 million tons of this residue available as surplus annually which projects it as a potent feedstock for conversion to bioethanol. The cellulose, hemicellulose and lignin content of t...

journal_title：Applied biochemistry and biotechnology

authors： Preeti VE,Sandhya SV,Kuttiraja M,Sindhu R,Vani S,Kumar SR,Pandey A,Binod P

更新日期：2012-07-01 00:00:00

abstract：:The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompeti...

journal_title：Applied biochemistry and biotechnology

authors： Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

更新日期：2012-01-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...

journal_title：Applied biochemistry and biotechnology

authors： Nguyen H,Pham T,Nguyen HL,Phan T

更新日期：2018-12-01 00:00:00

abstract：:RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not ...

journal_title：Applied biochemistry and biotechnology

authors： Nübel C,Appel B,Hospach I,Mai M,Krasteva N,Nelles G,Petruschka L,Müller S

更新日期：2016-06-01 00:00:00

abstract：:The RNA-guided endonuclease clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) derived from CRISPR systems is a simple and efficient genome-editing technology applied to various cell types and organisms. So far, the extensive approach to detect the cleavage activity of custo...

journal_title：Applied biochemistry and biotechnology

authors： Yang Y,Liu S,Cheng Y,Nie L,Lv C,Wang G,Zhang Y,Hao L

更新日期：2016-10-01 00:00:00

abstract：:Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This ...

journal_title：Applied biochemistry and biotechnology

authors： Jonas R,Silveira MM

更新日期：2004-07-01 00:00:00

abstract：:The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs proliferation has yet been reported. In the present study, the feas...

journal_title：Applied biochemistry and biotechnology

authors： Yu Y,Li K,Bao C,Liu T,Jin Y,Ren H,Yun W

更新日期：2009-10-01 00:00:00

abstract：:Six yeast strains of the commercial brewing yeasts CICC1391 and CICC1471, the commercial baker yeasts CICC1339 and CICC1447, and the commercial alcohol yeasts CICC1286 and CICC1291 have been cultured under 1.0 MPa of pressure with N(2) and CO(2) as pressure media. The concentration of intracellular trehalose and the a...

journal_title：Applied biochemistry and biotechnology

authors： Qiao C,Jia S,Dai Y,Wang R,Sun A

更新日期：2010-01-01 00:00:00

abstract：:The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which...

journal_title：Applied biochemistry and biotechnology

authors： Moayedi A,Mora L,Aristoy MC,Hashemi M,Safari M,Toldrá F

更新日期：2017-01-01 00:00:00

abstract：:Phosphoinositide 3-kinase (PI3K) enzyme plays a vital role in the insulin signaling pathway as well as in other pathways that are involved in the growth, migration, and survival of cells. In the insulin signaling pathway, PI3K proteins that include p50α, p85α, p85β, p55γ, p110α, p110β, and p110γ are associated with th...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty C,Doss CG,Bhatia R,Agoramoorthy G

更新日期：2015-04-01 00:00:00

abstract：:Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

更新日期：2018-01-01 00:00:00

abstract：:Ras-related domain family member 1 transcript variant A (RASSF1A) controls apoptosis and cell proliferation while p14/ARF gene has a regulatory role in cellular senescence. Failure of apoptosis and cellular senescence occurs during inflammatory bowel disease (IBD) and colorectal cancer (CRC). To reveal the role of per...

journal_title：Applied biochemistry and biotechnology

authors： Salama RH,Sayed ZEA,Ashmawy AM,Elsewify WA,Ezzat GM,Mahmoud MA,Alsanory AA,Alsanory TA

更新日期：2019-09-01 00:00:00

abstract：:The first step involved in the degradation of phthalate isomers (phthalate, isophthalate and terephthalate) is the double hydroxylation by respective aromatic-ring hydroxylating dioxygenases. These are two component enzymes consisting of 'oxygenase' and 'reductase' components. Soil isolate Pseudomonas aeruginosa strai...

journal_title：Applied biochemistry and biotechnology

authors： Karandikar R,Badri A,Phale PS

更新日期：2015-09-01 00:00:00

abstract：:Indole-3-carbinol (I3C) is a naturally occurring glucosinolate found in Brassica vegetables that is usually converted in gastric acidic environment to the efficient metabolite 3,3'-diindolylmethane (DIM). Both indoles (I3C and DIM) are known chemopreventive agents for various cancers including breast cancer. This stud...

journal_title：Applied biochemistry and biotechnology

authors： El-Daly SM,Gamal-Eldeen AM,Gouhar SA,Abo-Elfadl MT,El-Saeed G

更新日期：2020-12-01 00:00:00

abstract：:RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in com...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran PV,Ignacimuthu S

更新日期：2013-03-01 00:00:00

abstract：:The currently used multistep chemical synthesis for making surfaces antimicrobial by attaching to them hydrophobic polycations is replaced herein by an aerosol-assisted plasma deposition procedure. To this end, N,N-hexyl,methyl-PEI (HMPEI) is directly plasma-coated onto a glass surface. The resultant immobilized HMPEI...

journal_title：Applied biochemistry and biotechnology

authors： Liu H,Kim Y,Mello K,Lovaasen J,Shah A,Rice N,Yim JH,Pappas D,Klibanov AM

更新日期：2014-02-01 00:00:00

abstract：:An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish peroxidase, 4-amino-phenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis, with free and immobilized enzymes. The system with free enzymes pe...

journal_title：Applied biochemistry and biotechnology

authors： Alhadeff EM,Salgado AM,Pereira N Jr,Valdman B

更新日期：2004-04-01 00:00:00