Ex vitro expansion of human placenta-derived mesenchymal stem cells in stirred bioreactor.

abstract：:Efficient degradation of cellulose needs a synergistic reaction of the cellulolytic enzymes, which include exoglucanases, endoglucanases, and β-1,4-glucosidase. In this study, we used an improved Bac-to-Bac/BmNPV baculovirus expression system, which lacks the virus-encoded chitinase cathepsin (v-cath) genes of Bombyx ...

journal_title：Applied biochemistry and biotechnology

authors： Li XH,Wang MX,Zhang P,Hu JB,Sun CG,Liu XJ,Zhou F,Niu YS,Malik FA,Bhaskar R,Yang HJ,Miao YG

更新日期：2011-09-01 00:00:00

abstract：:Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for...

journal_title：Applied biochemistry and biotechnology

authors： Kazeem MO,Shah UKM,Baharuddin AS,AbdulRahman NA

更新日期：2017-08-01 00:00:00

abstract：:Over the last two decades many strategies have been planned to design specific drugs for rare diseases to target their action at the DNA level. Advancements in our understanding of the interactions of small nonpeptide molecules with DNA have opened the doors for "rational" drug design. Special methods have now been de...

journal_title：Applied biochemistry and biotechnology

authors： Prabhakar P,Kayastha AM

更新日期：1994-04-01 00:00:00

abstract：:meso-2,3-Butanediol (meso-2,3-BDO) is essential for the synthesis of various economically valuable biosynthetic products; however, the production of meso-2,3-BDO from expensive carbon sources is an obstacle for industrial applications. In this study, genes involved in the synthesis of 2,3-BDO in Klebsiella pneumoniae ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Kim B,Park K,Um Y,Lee J

更新日期：2012-04-01 00:00:00

abstract：:This study deals with the utilization of chicken feather waste as a substrate for anaerobic digestion and improving biogas production by degradation of the compact structure of the feather keratin. In order to increase the digestibility of the feather, different pretreatments were investigated, including thermal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Forgács G,Lundin M,Taherzadeh MJ,Sárvári Horváth I

更新日期：2013-04-01 00:00:00

abstract：:γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC 4.1.1.15) is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...

journal_title：Applied biochemistry and biotechnology

authors： Xiong Q,Xu Z,Xu L,Yao Z,Li S,Xu H

更新日期：2017-12-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate w...

journal_title：Applied biochemistry and biotechnology

authors： Kumar K,Bhargava P,Roy U

更新日期：2011-08-01 00:00:00

abstract：:The separation and purification of biologically functional molecules (e.g., proteins, antibodies, peptides, hormones, low molecular weight biologicals) is of fundamental importance to biotechnology. Affinity separations have become a particularly attractive method for bioseparations due to their high degree of selecti...

journal_title：Applied biochemistry and biotechnology

authors： Dordick JS

更新日期：1991-01-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:A hyperthermophilic alpha-amylase encoding gene from Pyrococcus woesei was transferred and expressed in Xanthomonas campestris ATCC 13951. The heterologous alpha-amylase activity was detected in the intracellular fraction of X. campestris and presented similar thermostability and catalytic properties with the native P...

journal_title：Applied biochemistry and biotechnology

authors： Konsoula Z,Liakopoulou-Kyriakides M,Perysinakis A,Chira P,Afendra A,Drainas C,Kyriakidis DA

更新日期：2008-05-01 00:00:00

abstract：:The effects of oxygen limitation on xylose fermentation of Neurospora crassa AS3.1602 were studied using batch cultures. The maximum yield of ethanol was 0.34 g/g at oxygen transfer rate (OTR) of 8.4 mmol/L.h. The maximum yield of xylitol was 0.33 g/g at OTR of 5.1 mmol/L.h. Oxygen limitation greatly affected mycelia ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Z,Qu Y,Zhang X,Lin J

更新日期：2008-03-01 00:00:00

abstract：:Steviol glycosides (SVglys) and gibberellins are originated from the shared biosynthesis pathway in Stevia (Stevia rebaudiana Bertoni). In this research, two experiments were conducted to study the opposing effects of external gibberellin (GA3) and Daminozide (a gibberellin inhibitor) on Stevia growth and metabolites....

journal_title：Applied biochemistry and biotechnology

authors： Karimi M,Hashemi J,Ahmadi A,Abbasi A,Pompeiano A,Tavarini S,Guglielminetti L,Angelini LG

更新日期：2015-01-01 00:00:00

abstract：:Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

journal_title：Applied biochemistry and biotechnology

authors： Ramya LN,Doble M,Rekha VP,Pulicherla KK

更新日期：2012-08-01 00:00:00

abstract：:Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...

journal_title：Applied biochemistry and biotechnology

authors： Cai Y,Liang X,Liao X,Ding Y,Sun J,Li X

更新日期：2010-04-01 00:00:00

abstract：:Hydrolysates were obtained from dilute sulfuric acid pretreatment of whole-tree softwood forest thinnings and softwood sawdust. Mid-infrared (IR) spectra were obtained on sample sets of wet washed hydrolysates, and 45 degrees C vacuum-dried washed hydrolysates, using a Fourier transform infrared (FTIR) spectrophotomet...

journal_title：Applied biochemistry and biotechnology

authors： Tucker MP,Nguyen QA,Eddy FP,Kadam KL,Gedvilas LM,Webb JD

更新日期：2001-04-01 00:00:00

abstract：:We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of tyrosinase. TFE increased the degree of secondary structure of tyrosinase, which directly resulted in enzyme inactivation. A reciprocal study showed that TFE inhibited tyrosinase in a slope-parabolic mixed-type inhibition m...

journal_title：Applied biochemistry and biotechnology

authors： Lü ZR,Shi L,Wang J,Park D,Bhak J,Yang JM,Park YD,Zhou HW,Zou F

更新日期：2010-04-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:Polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. In this study, specific bacterial strains produced specific copolymers from food waste. Copolymers of HB and HV (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]...

journal_title：Applied biochemistry and biotechnology

authors： Wong PA,Chua H,Lo W,Lawford HG,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

journal_title：Applied biochemistry and biotechnology

authors： Rondags E,Halliday E,Marc I

更新日期：1998-02-01 00:00:00

abstract：:The cellulolytic fungus, Trichoderma has oval and mononucleate conidia. When these conidia are incubated in a liquid medium, they begin to swell and their shape becomes spherical followed by an increase in inner space. In such swollen conidia, it is possible to produce a larger autopolyploid nucleus using a mitotic ar...

journal_title：Applied biochemistry and biotechnology

authors： Toyama H,Yano M,Hotta T

更新日期：2004-04-01 00:00:00

abstract：:A new colorimetric chemosensor for naked-eye detection and determination of cysteine (Cys) based on indicator displacement assay (IDA) was designed using 1-(2-pyridylazo)-2-naphthol (PAN). The indicator exchange occurred between PAN and Cys by the addition of Cys to the Cu(PAN)2 complex, which is accomplished by an im...

journal_title：Applied biochemistry and biotechnology

authors： Deilamy-Rad G,Asghari K,Tavallali H

更新日期：2020-09-01 00:00:00

abstract：:A new lipase from seeds of Pachira aquatica was purified to homogeneity by SDS-PAGE obtaining an enzyme with a molecular weight of approximately 55 kDa. The purified lipase exhibited maximum activity at 40 degrees C and pH 8.0, for an incubation time of 90 min. Concerning temperature stability, at the range from 4 to ...

journal_title：Applied biochemistry and biotechnology

authors： Polizelli PP,Facchini FD,Cabral H,Bonilla-Rodriguez GO

更新日期：2008-09-01 00:00:00

abstract：:Environmental conditions affect the production of extracellular polysaccharide by Azotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored expopolysaccharide production, which reache...

journal_title：Applied biochemistry and biotechnology

authors： De la Vega MG,Cejudo FJ,Paneque A

更新日期：1991-09-01 00:00:00

abstract：:In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high ac...

journal_title：Applied biochemistry and biotechnology

authors： Zhou HY,Pan HY,Rao LQ,Wu YY

更新日期：2011-01-01 00:00:00

abstract：:Ethanol from corn is produced using dry grind corn process in which simultaneous saccharification and fermentation (SSF) is one of the most critical unit operations. In this work an optimal controller based on a previously validated SSF model was developed by formulating the SSF process as a Bolza problem and using gr...

journal_title：Applied biochemistry and biotechnology

authors： Murthy GS,Johnston DB,Rausch KD,Tumbleson ME,Singh V

更新日期：2012-01-01 00:00:00

abstract：:The unique carbohydrate-binding property of lectins makes them invaluable tools in biomedical research. Here, we report the purification, partial primary structure, carbohydrate affinity characterization, crystallization, and preliminary X-ray diffraction analysis of a lactose-specific lectin from Cymbosema roseum see...

journal_title：Applied biochemistry and biotechnology

authors： Rocha BA,Moreno FB,Delatorre P,Souza EP,Marinho ES,Benevides RG,Rustiguel JK,Souza LA,Nagano CS,Debray H,Sampaio AH,de Azevedo WF Jr,Cavada BS

更新日期：2009-03-01 00:00:00

abstract：:Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

更新日期：2018-01-01 00:00:00

abstract：:The polysaccharides extracted from the achenes of jelly fig, Ficus awkeotsang Makino, were mainly composed of low methyl pectin and used as a novel shell material for encapsulating lipophilic bioactives in the core of microcapsule. The polysaccharide microcapsules with oil core were prepared using a novel acrylic-base...

journal_title：Applied biochemistry and biotechnology

authors： Ponrasu T,Yang RF,Chou TH,Wu JJ,Cheng YS

更新日期：2020-05-01 00:00:00