Highly Efficient and Rapid Detection of the Cleavage Activity of Cas9/gRNA via a Fluorescent Reporter.

abstract：:Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...

journal_title：Applied biochemistry and biotechnology

authors： Hinge V,Patil H,Nadaf A

更新日期：2016-02-01 00:00:00

abstract：:An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

journal_title：Applied biochemistry and biotechnology

authors： He X,Miao Y,Jiang X,Xu Z,Ouyang P

更新日期：2010-04-01 00:00:00

abstract：:Many lab-scale studies have been carried out regarding the effect of feed strategy on the performance of anaerobic sequencing batch reactors (ASBR); however, more detailed pilot-scale studies should be performed to assess the real applicability of this type of operation. Therefore, the objective of this work was to as...

journal_title：Applied biochemistry and biotechnology

authors： de Novaes LF,Borges LO,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-10-01 00:00:00

abstract：:Biomass degradation compounds significantly inhibit biochemical conversion of biomass prehydrolysates to biofuels and chemicals, such as lactic acid. To characterize the structure-activity relationship of carbonyl inhibition on lactic acid fermentation, we examined effects of eight carbonyl compounds (furfural, 5-hydr...

journal_title：Applied biochemistry and biotechnology

authors： Li J,Zhu C,Tu M,Han P,Wu Y

更新日期：2015-04-01 00:00:00

abstract：:To investigate the effect of pH and temperature on the cell growth and bacteriocin production of Pediococcus acidilactici PA003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of P. acidilactici PA003 was simulated in vitro during laboratory fermentations by making use of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Zhang Y,Liu SN,Han Y,Zhou ZJ

更新日期：2012-03-01 00:00:00

abstract：:To provide a mathematical basis for the design and operation of a continuous, packed-bed reactor for the interesterification of soybean oil, soybean oil that contains 22.7% oleoyl and 54.3% linoleoyl moieties as molar acyl moiety composition was interesterified in hexane with oleic acid, using an immobilized sn-1,3-sp...

journal_title：Applied biochemistry and biotechnology

authors： Akimoto M,Nagashima Y,Sato D

更新日期：1999-08-01 00:00:00

abstract：:The increased additive amount of adjuncts in the raw materials of Chinese beer requires the usage of protease to release more water-soluble proteins. Here, a metallo-neutral protease suited for brewing industry was purified from Bacillus amyloliquefaciens SYB-001. A 5.6-fold purification of the neutral protease was ac...

journal_title：Applied biochemistry and biotechnology

authors： Wang J,Xu A,Wan Y,Li Q

更新日期：2013-08-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...

journal_title：Applied biochemistry and biotechnology

authors： Xie R,Cui C,Chen B,Tan T

更新日期：2015-10-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a ...

journal_title：Applied biochemistry and biotechnology

authors： Wang X,Gong CS,Tsao GT

更新日期：1998-04-01 00:00:00

abstract：:An economic evaluation model was developed in the Laboratory of Agricultural Structures (LAS) of the Agricultural University of Athens-the Modified Basic Economic Evaluation Model (MBEEM). This model is an improved version of the original Basic Economic Evaluation Model, available in LAS, and it is used to assess the ...

journal_title：Applied biochemistry and biotechnology

authors： Georgakakis D,Christopoulou N,Chatziathanassiou A,Venetis T

更新日期：2003-04-01 00:00:00

abstract：:The unique carbohydrate-binding property of lectins makes them invaluable tools in biomedical research. Here, we report the purification, partial primary structure, carbohydrate affinity characterization, crystallization, and preliminary X-ray diffraction analysis of a lactose-specific lectin from Cymbosema roseum see...

journal_title：Applied biochemistry and biotechnology

authors： Rocha BA,Moreno FB,Delatorre P,Souza EP,Marinho ES,Benevides RG,Rustiguel JK,Souza LA,Nagano CS,Debray H,Sampaio AH,de Azevedo WF Jr,Cavada BS

更新日期：2009-03-01 00:00:00

abstract：:Human acidic fibroblast growth factor (haFGF) is a multifunctional protein involved in regulating a wide range of cellular processes. As a potent therapeutic agent, it is highly desirable to produce recombinant haFGF (r-haFGF) at low cost. However, the complex structure and formation of aggregation confines its high-l...

journal_title：Applied biochemistry and biotechnology

authors： Rahman IU,Liu W,Wei Z,Fang L,Zheng X,Huang L,Xu Z

更新日期：2020-08-01 00:00:00

abstract：:This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

journal_title：Applied biochemistry and biotechnology

authors： Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

更新日期：2000-11-01 00:00:00

abstract：:Endoglucanase 5 (EG5) has been isolated from the strain of E. coli TG1 harboring recombinant plasmid pCU108, which contains the cel5 gene of C. thermocellum. The enzyme has been produced with 98-fold purification and a final yield of 27% by using subsequent twofold high performance ion-exchange chromatography on Mono ...

journal_title：Applied biochemistry and biotechnology

authors： Mosolova TP,Kalyuzhnyi SV,Varfolomeyev SD,Velikodvorskaya GA

更新日期：1993-07-01 00:00:00

abstract：:Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditio...

journal_title：Applied biochemistry and biotechnology

authors： Deliormanlı AM,Atmaca H

更新日期：2018-12-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

journal_title：Applied biochemistry and biotechnology

authors： Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

更新日期：2019-10-01 00:00:00

abstract：:Here, we demonstrate the micropropagation protocol of Argyrolobium roseum (Camb.), an endangered herb exhibiting anti-diabetic and immune-suppressant properties, and antioxidant enzymes pattern is evaluated. Maximum callogenic response (60 %) was observed from leaf explant at 1.0 mg L(-1) 1-nephthalene acetic acid (NA...

journal_title：Applied biochemistry and biotechnology

authors： Habib D,Chaudhary MF,Zia M

更新日期：2014-01-01 00:00:00

abstract：:In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increa...

journal_title：Applied biochemistry and biotechnology

authors： Wu XQ,Wang J,Lü ZR,Tang HM,Park D,Oh SH,Bhak J,Shi L,Park YD,Zou F

更新日期：2010-03-01 00:00:00

abstract：:UDPG pyrophosphatase (UGPase) plays an important role in carbohydrate metabolism, catalyzing a reversible production of uridine diphosphate glucose (UDPG) and pyrophosphate (PPi) from Glc-1-P and UTP. UGPase gene from Aureobasidium pullulans NRRL Y-12974 was cloned, overexpressed in Escherichia coli. The recombinant U...

journal_title：Applied biochemistry and biotechnology

authors： Li H,Zhang Y,Gao Y,Lan Y,Yin X,Huang L

更新日期：2016-03-01 00:00:00

abstract：:Three yeast strains were isolated from decaying wood of Chilean Valdivian forest and identified as Meyerozyma guilliermondii, Scheffersomyces coipomensis, and Sugiyamaella paludigena. These strains were able to efficiently grow on the major monomers contained in Pinus spp. and Eucalyptus spp. wood that includes glucos...

journal_title：Applied biochemistry and biotechnology

authors： Valdés G,Mendonça RT,Parra C,Aggelis G

更新日期：2020-12-01 00:00:00

abstract：:Proteins that are important indicators of physiological or pathological states may contribute to the early diagnosis of disease, which may provide a basis for identifying the underlying mechanism of disease development. Serum, contains an abundance of proteins, offers an easy and inexpensive approach for disease detec...

journal_title：Applied biochemistry and biotechnology

authors： Zhang AH,Sun H,Yan GL,Han Y,Wang XJ

更新日期：2013-06-01 00:00:00

abstract：:The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Jones F,Bailey R,Wilson S,Hiestand J

更新日期：2007-04-01 00:00:00

abstract：:Fatty acid methyl esters (FAMEs) show large potential applications as diesel substitutes, also known as biodiesel fuel. Biodiesel fuel as renewable energy is an alternative that can reduce energy dependence on petroleum as well as air pollution. Several processes for the production of biodiesel fuel have been develope...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Kang CH,Lee WT,Sunwoo CS,Yoon CH,Choi BC,Kim HS,Kim SW,Lee UT

更新日期：2004-04-01 00:00:00

abstract：:In this paper, Ni/β-Mo2C(S) and Ni/β-Mo2C(G) were prepared from solution-derived precursor with two different carbon sources (starch and glucose) and tested as anodic noble-metal-free catalysts in air-cathode microbial fuel cells (MFCs). The carburized catalyst samples were characterized by X-ray diffraction (XRD), sc...

journal_title：Applied biochemistry and biotechnology

authors： Zeng LZ,Zhao SF,Li WS

更新日期：2015-06-01 00:00:00

abstract：:The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...

journal_title：Applied biochemistry and biotechnology

authors： Nguyen H,Pham T,Nguyen HL,Phan T

更新日期：2018-12-01 00:00:00

abstract：:The glpX gene from Francisella tularensis encodes for the class II fructose 1,6-bisphosphatase (FBPaseII) enzyme. The glpX gene has been verified to be essential in F. tularensis, and the inactivation of this gene leads to impaired bacterial growth on gluconeogenic substrates. In the present work, we have complemented...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Wolf NM,Bondoc JMG,Movahedzadeh F

更新日期：2017-12-01 00:00:00

abstract：:Fresh-harvested, air-dried rice straw was pretreated at a water content of 5 g H(2)O/g straw using sodium hydroxide (NaOH) and compared to pretreatment at 10 g H(2)O/g straw by hydrated lime (Ca(OH)(2)). Full factorial experiments including parallel wash-only treatments were completed with both sources of alkali. The ...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YS,Zheng Y,Yu CW,Dooley TM,Jenkins BM,VanderGheynst JS

更新日期：2010-11-01 00:00:00

abstract：:The effect of temperature on the performance of an anaerobic sequencing biofilm batch reactor (ASBBR) with liquid-phase recirculation was assessed. Assays were performed using a recirculation velocity of 0.20 cm/s, 8-h cycles, and an average treated synthetic wastewater volume of 2 L/cycle with a concentration of 500 ...

journal_title：Applied biochemistry and biotechnology

authors： Agibert SA,Moreira MB,Ratusznei SM,Rodrigues JA,Zaiat M,Foresti E

更新日期：2007-02-01 00:00:00

abstract：:Six yeast strains of the commercial brewing yeasts CICC1391 and CICC1471, the commercial baker yeasts CICC1339 and CICC1447, and the commercial alcohol yeasts CICC1286 and CICC1291 have been cultured under 1.0 MPa of pressure with N(2) and CO(2) as pressure media. The concentration of intracellular trehalose and the a...

journal_title：Applied biochemistry and biotechnology

authors： Qiao C,Jia S,Dai Y,Wang R,Sun A

更新日期：2010-01-01 00:00:00