Kinetics of ergothioneine inhibition of mushroom tyrosinase.

Abstract:

:The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompetitive tyrosinase inhibitor using a Lineweaver-Burk plot of experimental kinetic data. The IC(50) value for ergothioneine scavenging of 2,2-diphenyl-1-picrylhydrazyl was 6.110 ± 0.305 mg/ml, much higher than the IC(50) for inhibition of tyrosinase activity which indicating ergothioneine on tyrosinase shows a weak correlation to its antioxidative activity. The results demonstrated that ergothioneine has a potent inhibition effect on tyrosinase enzyme activity, resulting from the presence of the sulfur substituted imidazole ring in ergothioneine.

authors

Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

doi

10.1007/s12010-011-9421-x

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

259-67

issue

2

eissn

0273-2289

issn

1559-0291

journal_volume

166

pub_type

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