Evaluation of recombinant green fluorescent protein, under various culture conditions and purification with HiTrap hydrophobic interaction chromatography resins.

abstract：:A new label--laccase from the fungus Coriolus hirsutus--was applied for solid-phase enzyme-linked immunosorbent assays of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D). Two proposed assays are based on (1) competitive binding of antibody-laccase conjugate with immobilized 2,4-D-protein conjugate and 2,4-D in te...

journal_title：Applied biochemistry and biotechnology

authors： Zherdev AV,Bizova NA,Yaropolov AI,Lyubimova NV,Morozova OV,Dzantiev BB

更新日期：1999-03-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

journal_title：Applied biochemistry and biotechnology

authors： MacCallum JD,Hefford MA,Omar S,Beauregard M

更新日期：1997-04-01 00:00:00

abstract：:An anaerobic sequencing batch reactor containing immobilized biomass (AnSBBR) was used to produce biomethane by treating the effluent from another AnSBBR used to produce biohydrogen from glucose- (AR-EPHG) and sucrose-based (AR-EPHS) wastewater. In addition, biomethane was also produced from sucrose-based synthetic wa...

journal_title：Applied biochemistry and biotechnology

authors： Lullio TG,Souza LP,Ratusznei SM,Rodrigues JA,Zaiat M

更新日期：2014-11-01 00:00:00

abstract：:Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

journal_title：Applied biochemistry and biotechnology

authors： Aldin S,Tu F,Nakhla G,Ray MB

更新日期：2011-08-01 00:00:00

abstract：CONCLUSION:Immobilization of the thermophilic lactase causes a decrease in the formation of intermediary products. This means that partially hydrolyzed lactose syrups contain less of these intermediary products when the immobilized thermophilic lactase is used in the hydrolysis process than when a soluble lactase is us...

journal_title：Applied biochemistry and biotechnology

authors： Rugh S

更新日期：1982-01-01 00:00:00

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...

journal_title：Applied biochemistry and biotechnology

authors： Xie R,Cui C,Chen B,Tan T

更新日期：2015-10-01 00:00:00

abstract：:Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

journal_title：Applied biochemistry and biotechnology

authors： Bresolin ITL,Bresolin IRAP,Bueno SMA

更新日期：2020-06-01 00:00:00

abstract：:Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

journal_title：Applied biochemistry and biotechnology

authors： Rodriguez C,Nam DH,Kruchowy E,Ge X

更新日期：2017-10-01 00:00:00

abstract：:Marinobacter sp. (MSI032) isolated from the marine sponge Dendrilla nigra was optimized for the production of extracellular cellulolytic enzyme (CMCase) by submerged fermentation. Initial experiments showed that the culture medium containing 1% maltose as carbon source and 1% peptone and casein as nitrogen source supp...

journal_title：Applied biochemistry and biotechnology

authors： Shanmughapriya S,Kiran GS,Selvin J,Thomas TA,Rani C

更新日期：2010-10-01 00:00:00

abstract：:Seven white rot fungal species were tested for growth as mycelia using swine wastewater (SW), an agro-waste with tremendous environmental footprint, as the sole nutrient source. The SW contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. Out...

journal_title：Applied biochemistry and biotechnology

authors： Lee J,Shin SG,Ahn J,Han G,Hwang K,Kim W,Hwang S

更新日期：2017-02-01 00:00:00

abstract：:Fuel ethanol can be produced from softwood through hydrolysis in an enzymatic process. Prior to enzymatic hydrolysis of the softwood, pretreatment is necessary. In this study, two-step steam pretreatment employing dilute H2SO4 impregnation in the first step and SO2 impregnation in the second step, to improve the overa...

journal_title：Applied biochemistry and biotechnology

authors： Söderström J,Pilcher L,Galbe M,Zacchi G

更新日期：2003-04-01 00:00:00

abstract：:The characteristics of the new nitrilase-producing strain Rhodobacter sphaeroides LHS-305 were investigated. By investigating several parameters influencing nitrilase production, the specific cell activity was ultimately increased from 24.5 to 75.0 μmol g(-1) min(-1), and hereinto, the choice of inducer proved the mos...

journal_title：Applied biochemistry and biotechnology

authors： Yang C,Wang X,Wei D

更新日期：2011-12-01 00:00:00

abstract：:Glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase is an enzyme that converts GL-7-ACA to 7-aminocephalosporanic acid, a starting material for semisynthetic cephalosporin antibiotics. In this study, optimal conditions for the immobilization of GL-7-ACA acylase were determined by experimental observations and stat...

journal_title：Applied biochemistry and biotechnology

authors： Park SW,Lee JW,Hong SI,Kim SW

更新日期：2003-03-01 00:00:00

abstract：:Cancer is one of the killer diseases in humans and needs alternate curative measures despite recent improvement in modern treatment modalities. Oncolytic virotherapy seems to be a promising nonconventional way to treat cancers. Newcastle disease virus (NDV), a poultry virus, is nonpathogenic to human and domestic anim...

journal_title：Applied biochemistry and biotechnology

authors： Kumar R,Tiwari AK,Chaturvedi U,Kumar GR,Sahoo AP,Rajmani RS,Saxena L,Saxena S,Tiwari S,Kumar S

更新日期：2012-08-01 00:00:00

abstract：:Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

journal_title：Applied biochemistry and biotechnology

authors： Clark KJ,Harcum SW,Griffiths J,Bailey KM

更新日期：2005-06-01 00:00:00

abstract：:In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

journal_title：Applied biochemistry and biotechnology

authors： Silva-Jiménez H,Zazueta-Sandoval R

更新日期：2005-04-01 00:00:00

abstract：:The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical...

journal_title：Applied biochemistry and biotechnology

authors： Gou L,Zhan Y,Lee J,Li X,Lü ZR,Zhou HM,Lu H,Wang XY,Park YD,Yang JM

更新日期：2015-02-01 00:00:00

abstract：:Microalgae have tremendous potential as a renewable feedstock for the production of liquid transportation fuels. In natural waters, the importance of physical associations and biochemical interactions between microalgae and bacteria is generally well appreciated, but the significance of these interactions to algal bio...

journal_title：Applied biochemistry and biotechnology

authors： Bagwell CE,Piskorska M,Soule T,Petelos A,Yeager CM

更新日期：2014-08-01 00:00:00

abstract：:This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

journal_title：Applied biochemistry and biotechnology

authors： Sun ZL,Sun LQ,Chen GZ

更新日期：2019-04-01 00:00:00

abstract：:Asphaltenes from a crude oil rich in heavy metals (Castilla crude oil) were fractionated and partially characterized. Biocatalytic modifications of these fractionated asphaltenes by three different hemoproteins: chloro-peroxidase (CPO), cytochrome C peroxidase (Cit-C), and lignin peroxidase (LPO) were evaluated in bot...

journal_title：Applied biochemistry and biotechnology

authors： Mogolloń L,Rodríguez R,Larrota W,Ortiz C,Torres R

更新日期：1998-04-01 00:00:00

abstract：:Prostate apoptosis response-4 (Par-4), an anticancer protein that interacts with cell surface receptor GRP78, can selectively suppress proliferation and induce apoptosis of cancer cells. The core domain of Par-4 (aa 137-195), designated as SAC, is sufficient to inhibit tumor growth and metastasis without harming norma...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Sun A,Dong Y,Wei D

更新日期：2018-04-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liq...

journal_title：Applied biochemistry and biotechnology

authors： Du L,Loha V,Tanner RD

更新日期：2000-04-01 00:00:00

abstract：:Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

journal_title：Applied biochemistry and biotechnology

authors： Ferreira MS,De Andrade AV,Kennedy JF

更新日期：1991-10-01 00:00:00

abstract：:Gene expression pattern of two important regulatory proteins, salt overly sensitive 2 (SOS2) and plasma membrane protein 3-1 (PMP3-1), involved in ion homeostasis, was analyzed in two salinity-contrasting sunflower (Helianthus annuus L.) lines, Hysun-38 (salt tolerant) and S-278 (moderately salt tolerant). The pattern...

journal_title：Applied biochemistry and biotechnology

authors： Saadia M,Jamil A,Ashraf M,Akram NA

更新日期：2013-06-01 00:00:00

abstract：:Using microalgae to capture CO2 from flue gas is an ideal way to reduce CO2 emission, but this is challenged by the high cost of carbon capture and transportation. To address this problem, a bicarbonate-based integrated carbon capture and algae production system (BICCAPS) has been proposed, in which bicarbonate is use...

journal_title：Applied biochemistry and biotechnology

authors： Chi Z,Elloy F,Xie Y,Hu Y,Chen S

更新日期：2014-01-01 00:00:00

abstract：:Adenosine monophosphate-activated protein kinase (AMPK) is viewed as a privileged therapeutic target for several diseases such as cancer, diabetes, inflammation, obesity, etc. In addition, AMPK has entered the limelight of current drug discovery with its evolution as a key metabolic regulator. AMPK also plays a key ro...

journal_title：Applied biochemistry and biotechnology

authors： Ramesh M,Vepuri SB,Oosthuizen F,Soliman ME

更新日期：2016-02-01 00:00:00

abstract：:Growth kinetics and red pigment production of Monascus purpureus CCT 3802 was studied. A reproducible inoculum with extremely dispersed hyphae for bioreactor runs was obtained through a two-step cultivation in a shaker. First, the spores were cultivated in a complex medium rendering a suspension of vegetative cells. I...

journal_title：Applied biochemistry and biotechnology

authors： Pereira DG,Kilikian BV

更新日期：2001-04-01 00:00:00