Properties of a thermostable nonspecific fructofuranosidase produced by Cladosporium cladosporioides cells for hydrolysis of Jerusalem artichoke extract.

abstract：:Sorghum was pretreated by sole ultrasound or supercritical carbon dioxide (scCO2), as well as the method combining both to intensify enzymatic hydrolysis. The effect of the time (1-5 h) and temperature (30-70 °C) on ultrasonic pretreatment was investigated, and the best condition was determined as 5 h and 50 °C with t...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zhao M,Xu Q,Ren H,Yin J

更新日期：2019-05-01 00:00:00

abstract：:A comparative proteomic analysis was carried out to explore the molecular mechanisms of responses to cold stress in Phalaenopsis after treated by low temperature (13/8 °C day/night) for 15 days. Differentially expressed proteins were examined using two-dimensional electrophoresis (2-DE) and matrix assisted laser desor...

journal_title：Applied biochemistry and biotechnology

authors： Yuan XY,Liang F,Jiang SH,Wan MF,Ma J,Zhang XY,Cui B

更新日期：2015-01-01 00:00:00

abstract：:In this study, immobilization conditions and bioethanol production characteristics of immobilized Saccharomyces bayanus were investigated into sodium alginate-graft-poly(N-vinyl-2-pyrrolidone; NaAlg-g-PVP) matrix. The matrix that crosslinked with calcium clorid was used for immobilization of S. bayanus. Bioethanol pro...

journal_title：Applied biochemistry and biotechnology

authors： İnal M,Yiğitoğlu M

更新日期：2012-09-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00

abstract：:A newly isolated bacterial strain, Bacillus sp. MX47, was actively producing extracellular xylanase only in xylan-containing medium. The xylanase was purified from the culture broth by two chromatographic steps. The xylanase had an apparent molecular weight of 26.4 kDa with an NH(2)-terminal sequence (Gln-Gly-Gly-Asn-...

journal_title：Applied biochemistry and biotechnology

authors： Chi WJ,Park DY,Chang YK,Hong SK

更新日期：2012-10-01 00:00:00

abstract：:The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixtur...

journal_title：Applied biochemistry and biotechnology

authors： Huang Z,Li Z,Su Y,Zhu Y,Zeng W,Chen G,Liang Z

更新日期：2018-08-01 00:00:00

abstract：:Candida boidinii produces significant amounts of xylitol from xylose, and assays of crude homogenates for aldose (xylose) reductase (XYL1p) have been reported to show relatively high activity with NADH as a cofactor even though XYL1p purified from this yeast does not have such activity. A gene coding for XYL1p from C....

journal_title：Applied biochemistry and biotechnology

authors： Kang MH,Ni H,Jeffries TW

更新日期：2003-04-01 00:00:00

abstract：:Light oil from pyrolysis, which accounts for ∼10 % carbon yield of the starting biomass, is a complex aqueous product that is difficult to utilize and usually discarded. This work presents the feasibility of light oil as a sole carbon source to support the growth of Rhodococcus opacus (R. opacus) that in turn accumula...

journal_title：Applied biochemistry and biotechnology

authors： Wei Z,Zeng G,Kosa M,Huang D,Ragauskas AJ

更新日期：2015-01-01 00:00:00

abstract：:Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

journal_title：Applied biochemistry and biotechnology

authors： Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

更新日期：2014-04-01 00:00:00

abstract：:Crosslinked enzyme aggregates (CLEAs) of a partially purified penicillin acylase from a recombinant Escherichia coli strain have been produced as a novel type of biocatalysts well endowed to perform in organic media. Different protein precipitants were studied and glutaraldehyde was used as the crosslinking agent. Pre...

journal_title：Applied biochemistry and biotechnology

authors： Illanes A,Wilson L,Caballero E,Fernández-Lafuente R,Guisán JM

更新日期：2006-06-01 00:00:00

abstract：:The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...

journal_title：Applied biochemistry and biotechnology

authors： Selma VC,Cotrim LH,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-12-01 00:00:00

abstract：:The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate...

journal_title：Applied biochemistry and biotechnology

authors： Chaubey A,Pande KK,Pandey MK,Singh VS

更新日期：2001-10-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:The semi-continuous anaerobic digestion of food waste was investigated in 1-L and 20-L continuously stirred tank reactors (CSTRs), to identify the optimum operation condition and the methane production of the semi-continuous anaerobic process. Results from a 1-L digester indicated that the optimum organic loading rate...

journal_title：Applied biochemistry and biotechnology

authors： Zhang C,Su H,Wang Z,Tan T,Qin P

更新日期：2015-04-01 00:00:00

abstract：:Cell-penetrating peptides are molecules with the ability to cross membranes and enter cells. Attention has been put on these peptides as a tool for drug delivery research, as they are able to serve as delivery vectors for large molecules. Intracellular delivery of bioactive peptides is a very promising research area f...

journal_title：Applied biochemistry and biotechnology

authors： Rodríguez V,Lascani J,Asenjo JA,Andrews BA

更新日期：2015-03-01 00:00:00

abstract：:In the present work, assays were performed to compare the efficacy of hypochlorous acid, chlorine dioxide, and ozone in the inactivation of Cryptosporidium oocyst in public water supply from Brazilian South conditions. Experiments were carried out in samples containing 2 x 10(4) oocysts/ml of C. parvum purified from f...

journal_title：Applied biochemistry and biotechnology

authors： Pereira JT,Costa AO,de Oliveira Silva MB,Schuchard W,Osaki SC,de Castro EA,Paulino RC,Soccol VT

更新日期：2008-12-01 00:00:00

abstract：:Bioreduction of 1-phenyl-2-propanone to prepare (S)-1-phenyl-2-propanol, a useful pharmaceutical intermediate, was performed with growing cells of Rhodococcus erythropolis JX-021, giving 14 mM (1.9 g/L) product in 99% e.e. at 5 h in the catalysis of 15 mM substrate. The reduction stopped afterwards due to strong inhib...

journal_title：Applied biochemistry and biotechnology

authors： Jin JZ,Li H,Zhang J

更新日期：2010-11-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs which play significant role in RNA interference. The present work deals with mining of the conserved miRNA and their target genes from the contigs, ESTs, and BAC end sequences of commercially important catfish, Clarias batrachus, from India. A total of 138, 1 and 1 conserved pre-miR...

journal_title：Applied biochemistry and biotechnology

authors： Agarwal S,Nagpure NS,Srivastava P,Kumar R,Pandey M,Srivastava S,Jena JK,Das P,Kushwaha B

更新日期：2017-07-01 00:00:00

abstract：:Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research. Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transfo...

journal_title：Applied biochemistry and biotechnology

authors： Dang X,Shan Z,Chen H

更新日期：2016-11-01 00:00:00

abstract：:Aspergillus niger CTBU isolated from local decayed bamboo shoot residue was employed to solid-state fermentation (SSF) of bamboo shoot residue to elevate the content of phytosterols. Strain acclimatization was carried out under the fermentation condition using bamboo shoot as substrate for fermentation performance imp...

journal_title：Applied biochemistry and biotechnology

authors： Zheng XX,Chen RS,Shen Y,Yin ZY

更新日期：2014-04-01 00:00:00

abstract：:The thermostable and organic solvent tolerant whole-cell lipase (WCL) was produced by Burkholderia sp. ZYB002 with broad spectrum organic solvent tolerance. The production medium of the WCL was primarily optimized, which resulted in the maximum activity of 22.8 U/mL and the 5.1-fold increase of the WCL yield. The opti...

journal_title：Applied biochemistry and biotechnology

authors： Shu ZY,Wu JG,Cheng LX,Chen D,Jiang YM,Li X,Huang JZ

更新日期：2012-02-01 00:00:00

abstract：:An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...

journal_title：Applied biochemistry and biotechnology

authors： de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde L

更新日期：2010-03-01 00:00:00

abstract：:An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...

journal_title：Applied biochemistry and biotechnology

authors： Yin C,Zheng L,Chen L,Tan Q,Shang X,Ma A

更新日期：2014-02-01 00:00:00

abstract：:Chicken feathers are available in large quantities around the world causing environmental challenges. The feathers are composed of keratin that is a recalcitrant protein and is hard to degrade. In this work, chicken feathers were aerobically pretreated for 2-8 days at total solid concentrations of 5, 10, and 20 % by B...

journal_title：Applied biochemistry and biotechnology

authors： Patinvoh RJ,Feuk-Lagerstedt E,Lundin M,Sárvári Horváth I,Taherzadeh MJ

更新日期：2016-12-01 00:00:00

abstract：:Nephelium lappaceum L., commonly known as "rambutan," is a typical tropical tree and is well known for its juicy and sweet fruit which has an exotic flavor. Chemical studies on rambutan have led to the identification of various components such as monoterpene lactones and volatile compounds. Here, a 22.5-kDa trypsin in...

journal_title：Applied biochemistry and biotechnology

authors： Fang EF,Ng TB

更新日期：2015-04-01 00:00:00

abstract：:An extracellular low molecular weight xylanase (Xyn628) from Streptomyces sp. CS628 was isolated from Korean soil sample, produced in wheat bran medium, purified, and biochemically characterized. Xyn628 was purified 4.8-fold with a 33.78 % yield using Sepharose CL-6B column chromatography. The purified xylanase was ~1...

journal_title：Applied biochemistry and biotechnology

authors： Rahman MA,Choi YH,Pradeep GC,Choi YS,Choi EJ,Cho SS,Yoo JC

更新日期：2014-07-01 00:00:00

abstract：:The aim of this work was to establish optimal conditions for the maximum production of endo-beta-1,4 mannanases using cheaper sources. Eight thermotolerant fungal strains were isolated from garden soil and compost samples collected in and around the Gulbarga University campus, India. Two strains were selected based on...

journal_title：Applied biochemistry and biotechnology

authors： Kote NV,Patil AG,Mulimani VH

更新日期：2009-02-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:More than two decades ago, a group of research engineers and applied scientists with interest in energy applications of bioprocessing initiated an annual symposium series ultimately entitled "Biotechnology for Fuels and Chemicals." The Department of Energy, several of the national laboratories, and various industrial ...

journal_title：Applied biochemistry and biotechnology

authors： Scott CD

更新日期：2004-04-01 00:00:00

abstract：:Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. Despite the wealth of information on abiotic stress and stress tolerance in plants, many aspects still remain unclear. Prosopis juliflora is a hardy plant reported to be tolerant to drought, salinity, extremes of soil pH...

journal_title：Applied biochemistry and biotechnology

authors： George S,Usha B,Parida A

更新日期：2009-05-01 00:00:00