Abstract：
:A comparative proteomic analysis was carried out to explore the molecular mechanisms of responses to cold stress in Phalaenopsis after treated by low temperature (13/8 °C day/night) for 15 days. Differentially expressed proteins were examined using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-TOF/MS). Among 85 differentially expressed proteins, 73 distinct proteins were identified. Comparative analysis revealed that the identified proteins mainly participate in photosynthesis, protein synthesis, folding and degradation, respiration, defense response, amino acid metabolism, energy pathway, cytoskeleton, transcription regulation, signal transduction, and seed storage protein, while the functional classification of the remaining four proteins was not determined. These data suggested that the proteins might work cooperatively to establish a new homeostasis under cold stress; 37 % of the identified cold-responsive proteins were associated with various aspects of chloroplast physiology, and 56 % of them were predicted to be located in the chloroplasts, implying that the cold stress tolerance of Phalaenopsis was achieved, at least partly, by regulation of chloroplast function. Moreover, the protein destination control, which was mediated by chaperones and proteases, plays an important role in tolerance to cold stress.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Yuan XY,Liang F,Jiang SH,Wan MF,Ma J,Zhang XY,Cui Bdoi
10.1007/s12010-014-1345-9subject
Has Abstractpub_date
2015-01-01 00:00:00pages
909-24issue
2eissn
0273-2289issn
1559-0291journal_volume
175pub_type
杂志文章abstract：:Chicken feathers are available in large quantities around the world causing environmental challenges. The feathers are composed of keratin that is a recalcitrant protein and is hard to degrade. In this work, chicken feathers were aerobically pretreated for 2-8 days at total solid concentrations of 5, 10, and 20 % by B...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2175-8
更新日期：2016-12-01 00:00:00
abstract：:This study was carried out to evaluate the capacity of anaerobic granular sludge for oxidizing ammonium and p-cresol with nitrate as terminal electron acceptor. Kinetics for the anaerobic oxidation of ammonium and p-cresol is described in this paper. The phenolic compound was very efficiently consumed, achieving 65 % ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1702-3
更新日期：2015-08-01 00:00:00
abstract：:A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), a...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-018-2906-0
更新日期：2019-05-01 00:00:00
abstract：:The aim of the present study was bioremediation of distillery sludge into a soil-enriching material. It was mixed with a complementary waste, cattle dung, and subjected to vermicomposting with (V) and without (T, control) Eisenia fetida in the ratio of 0:100 % (V1, T1), 10:90 (V2, T2), 25:75 (V3, T3), 50:50 (V4, T4), ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-1116-7
更新日期：2014-10-01 00:00:00
abstract：:A new bioprocess for production of sorbitol and gluconic acid from two low-cost feedstocks, inulin and cassava starch, using a commercially available enzyme was proposed in this study. The commercial glucoamylase GA-L NEW from Genencor was found to demonstrate a high inulinase activity for hydrolysis of inulin into fr...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0278-z
更新日期：2013-12-01 00:00:00
abstract：:We screened about a hundred microorganisms (including unidentified yeasts, fungi, and bacteria) for their ability to produce xylanolytic enzymes. About 40 of them were hemicellulolytic; among these, we selected Penicillium canescens 10-10c for detailed study because of its ability to produce an interesting enzymatic c...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02787840
更新日期：1997-07-01 00:00:00
abstract：:In this work, fibronectin purification from human plasma with the gelatin-immobilised poly(hydroxyethyl methacrylate) (PHEMA) cryogel has been evaluated. The PHEMA cryogel was prepared by cryo-polymerisation which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. The PHEMA cryogel contained i...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0352-6
更新日期：2013-09-01 00:00:00
abstract：:A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-012-9569-z
更新日期：2012-04-01 00:00:00
abstract：:In this study, starches obtained from wheat, potato, and corn were used to synthesize cationic starches (CS), and the flocculation efficiency of these materials was tested with Chlorella pyrenoidosa and Botryococcus braunii cultures under different conditions. Our results indicated that these three CS had differing de...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2202-9
更新日期：2017-01-01 00:00:00
abstract：:The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-012-9987-y
更新日期：2013-01-01 00:00:00
abstract：:The biosurfactant-producing Pseudomonas aeruginosa A11, with plant-growth-promoting (PGP) and multi-metal-resistant (MMR) features was isolated from the rhizosphere of a wild plant Parthenium hysterophorus. The strain A11 was able to utilize glycerol as a carbon source and produce 4,436.9 mg/L of biosurfactant after 1...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0244-9
更新日期：2013-07-01 00:00:00
abstract：:The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:78:1-3:401
更新日期：1999-04-01 00:00:00
abstract：:The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y,...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1937-z
更新日期：2016-03-01 00:00:00
abstract：:MicroRNAs are small non-coding RNAs which play significant role in RNA interference. The present work deals with mining of the conserved miRNA and their target genes from the contigs, ESTs, and BAC end sequences of commercially important catfish, Clarias batrachus, from India. A total of 138, 1 and 1 conserved pre-miR...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2373-4
更新日期：2017-07-01 00:00:00
abstract：:The fermentative production of lactic acid from cheese whey and corn steep liquor (CSL) as cheap raw materials was investigated by using Lactobacillus sp. RKY2 in order to develop a cost-effective fermentation medium. Lactic acid yields based on consumed lactose were obtained at more than 0.98 g/g from the medium cont...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-1037-5
更新日期：2014-09-01 00:00:00
abstract：:Calotropis procera latex fractions possessing anti-inflammatory property were characterized for their biochemical properties, compared for their efficacy in ameliorating fever in rats and their mechanism of action was elucidated. Aqueous fraction and methanol extract (AqDL and MeDL) were derived from the dried latex (...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2395-y
更新日期：2017-07-01 00:00:00
abstract：:The characteristics of the new nitrilase-producing strain Rhodobacter sphaeroides LHS-305 were investigated. By investigating several parameters influencing nitrilase production, the specific cell activity was ultimately increased from 24.5 to 75.0 μmol g(-1) min(-1), and hereinto, the choice of inducer proved the mos...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-011-9375-z
更新日期：2011-12-01 00:00:00
abstract：:To develop more potent thrombolytic agents with fibrinolytic and antiplatelet aggregation activity, staphylokinase (Sak) variant Y1-Sak, a recombinant mutant of the Staphylococcus aureus protein Sak, was constructed. Y1-Sak formed an insoluble inclusion body when overexpressed in Escherichia coli strain JF1125. To obt...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-008-8150-2
更新日期：2008-10-01 00:00:00
abstract：:Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantificatio...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-019-03179-8
更新日期：2020-05-01 00:00:00
abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2037-4
更新日期：2016-07-01 00:00:00
abstract：:Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for b...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-020-03258-1
更新日期：2020-05-01 00:00:00
abstract：:Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-017-2439-y
更新日期：2017-09-01 00:00:00
abstract：:An integrated biodiesel process that combines enzymatic esterification and alkaline transesterification is suggested. With focus on the enzymatic step, the paper provides proof of concept and suggestions for further process development. Hence, palm fatty acid distillate (PFAD) has been enzymatically converted to fatty...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-010-9095-9
更新日期：2011-04-01 00:00:00
abstract：:The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid an...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:117:1:01
更新日期：2004-04-01 00:00:00
abstract：:Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectrosc...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-020-03337-3
更新日期：2020-10-01 00:00:00
abstract：:Beta-xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x1), CTAB concentr...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:91-93:1-9:719
更新日期：2001-04-01 00:00:00
abstract：:Magnetosomes are specialized organelles arranged in intracellular chains in magnetotactic bacteria. The superparamagnetic property of these magnetite crystals provides potential applications as contrast-enhancing agents for magnetic resonance imaging. In this study, we compared two different nanoparticles that are bac...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-017-2642-x
更新日期：2018-05-01 00:00:00
abstract：:This article demonstrates the potential of encapsulated, engineered Lactococcus lactis as a vehicle for the oral delivery of therapeutic proteins. Using alginate-poly-l-lysine-alginate membrane-encapsulated L. lactis engineered to secrete the reporter protein Staphylococcal aureus nuclease, we show comparable viabilit...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-007-0002-y
更新日期：2007-07-01 00:00:00
abstract：:Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob. The immobilization is carried out by tosylation of primary alcohols of cellulose of corn cob. The immobilized benzonase is more stable vs pH changes than native benzonase, but the ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02922124
更新日期：1991-10-01 00:00:00
