Abstract:
:The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58-64% cellulose, 2-16% hemicellulose, and 24-30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200 degrees C for 10 min at neutral pH.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Palonen H,Thomsen AB,Tenkanen M,Schmidt AS,Viikari Ldoi
10.1385/abab:117:1:01subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1-17issue
1eissn
0273-2289issn
1559-0291pii
ABAB:117:1:01journal_volume
117pub_type
杂志文章abstract::The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denatur...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9195-1
更新日期:2011-08-01 00:00:00
abstract::Copper (II) oxide nanoparticles (CuO-NPs) have been studied as potential antimicrobial agents, similar to silver or platinum nanoparticles. However, the use of excess NPs is limited by their safety and toxicity in beneficial microflora and human cells. In this study, we evaluated the cytotoxicity of CuO-NPs by coating...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03108-9
更新日期:2020-02-01 00:00:00
abstract::Seeking for simple, rapid, and environmental-friendly routes to produce metal nanoparticles is quite attractive for various biotechnological applications. Biological synthesis method of silver nanoparticles has been found very promising due to their non-toxicity and simplicity. Here, the spores of Bacillus stratospher...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1055-3
更新日期:2014-09-01 00:00:00
abstract::A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-0843-0
更新日期:2014-05-01 00:00:00
abstract::During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:95:2:103
更新日期:2001-08-01 00:00:00
abstract::The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1771-3
更新日期:2015-10-01 00:00:00
abstract::Corn fiber is the fibrous by-product of wet-mill corn processing. It typically consists of about 20% starch, 14% cellulose, and 30% hemicellulose in the form of arabinoxylan. Crude corn fiber (CCF) was fractionated into de-starched corn fiber (DSCF), corn fiber with cellulose (CFC) enriched, and corn fiber arabinoxyla...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then appl...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03404-9
更新日期:2021-01-01 00:00:00
abstract::Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-016-2037-4
更新日期:2016-07-01 00:00:00
abstract::A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02922612
更新日期:1991-04-01 00:00:00
abstract::To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02783752
更新日期:1984-01-01 00:00:00
abstract::Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03266-1
更新日期:2020-05-01 00:00:00
abstract::Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:87:2:95
更新日期:2000-05-01 00:00:00
abstract::An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0598-z
更新日期:2014-02-01 00:00:00
abstract::Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob. The immobilization is carried out by tosylation of primary alcohols of cellulose of corn cob. The immobilized benzonase is more stable vs pH changes than native benzonase, but the ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02922124
更新日期:1991-10-01 00:00:00
abstract::Biomass degradation compounds significantly inhibit biochemical conversion of biomass prehydrolysates to biofuels and chemicals, such as lactic acid. To characterize the structure-activity relationship of carbonyl inhibition on lactic acid fermentation, we examined effects of eight carbonyl compounds (furfural, 5-hydr...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1533-2
更新日期:2015-04-01 00:00:00
abstract::In this study, seedling, callus, cell, and adventitious root of Glycyrrhiza uralensis Fisch. have been established. In order to find the best one for producing G. uralensis active constituents, triterpenoid saponins and flavonoids in native root and tissue cultures were determined, and the contents in different G. ura...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-0012-2
更新日期:2013-01-01 00:00:00
abstract::In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02922595
更新日期:1991-04-01 00:00:00
abstract::Potassium acetate is currently made by reacting petroleum-based acetic acid with potassium hydroxide. An alternate process, anaerobic fermentation of dextrose with Clostridium thermoaceticum, could be used and could possibly be cheaper. Growth characteristics and productivity of the fermentation were optimized to maxi...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/978-1-4612-2312-2_37
更新日期:1997-04-01 00:00:00
abstract::Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02920153
更新日期:1998-04-01 00:00:00
abstract::Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-010-9070-5
更新日期:2011-03-01 00:00:00
abstract::γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC 4.1.1.15) is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2506-4
更新日期:2017-12-01 00:00:00
abstract::The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02920473
更新日期:1997-04-01 00:00:00
abstract::Termitomyces fungi associated with fungus-growing termites are the edible mushrooms and can produce useful chemicals, enzymes, and volatile organic compounds (VOCs) that have both fuel and biological potentials. To this purpose, we examined the Termitomyces mycelial growth performance on various substrates, clarified ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03376-w
更新日期:2020-12-01 00:00:00
abstract::The emergence of nalidixic acid-resistant strains of Salmonella typhimurium remains to be a major public health problem. In particular, the substitution of Asn in place of Asp at the 87 loci in the GyrA of S. typhimurium was experimentally stated for nalidixic acid resistance. However, the data on the possible mechani...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1760-6
更新日期:2015-09-01 00:00:00
abstract::The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, a...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/ABAB:131:1:738
更新日期:2006-03-01 00:00:00
abstract::Scheffersomyces stipitis was cultivated in an optimized, controlled fed-batch fermentation for production of ethanol from glucose-xylose mixture. Effect of feed medium composition was investigated on sugar utilization and ethanol production. Studying influence of specific cell growth rate on ethanol fermentation perfo...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0100-y
更新日期:2013-03-01 00:00:00
abstract::Development and application of a system for real-time quantitative assessment of individual cell activities in a mixed culture system was investigated. This was based on a concept that the activities of individual cells in a mixed culture can be assessed if the cells are physically separated (in separate compartments)...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:80:1:51
更新日期:1999-04-01 00:00:00
abstract::Fed-batch method has shown a great promise in debottlenecking the high-solid enzymatic hydrolysis for the commercialization of cellulosic biosugar conversion for biofuel/biochemical production. To further improve enzymatic hydrolysis efficiency at high solid loading, fed-batch methods of green liquor-pretreated hardwo...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2633-y
更新日期:2018-04-01 00:00:00
abstract::The feasibility of using mixtures of C4 and C5 chain-length aliphatic alcohols from fusel oil as the bulk organic media for lipase-mediated synthesis of laurate esters was assessed. Reaction mixtures consisted of lauric acid, lipase, solvent (if added), and appropriate amount of fusel oil (previously dehydrated with i...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:79:1-3:817
更新日期:1999-04-01 00:00:00