Abstract:
:Seeking for simple, rapid, and environmental-friendly routes to produce metal nanoparticles is quite attractive for various biotechnological applications. Biological synthesis method of silver nanoparticles has been found very promising due to their non-toxicity and simplicity. Here, the spores of Bacillus stratosphericus isolated from soil enriched with 30 % H2O2 were used for the production of silver nanoparticles. Furthermore, the possible mechanism of silver nanoparticle synthesis by the spores was elucidated for the first time. In this regard, dipicolinic acid (DPA) was shown to play a critical role as a nanoparticle-producing agent. UV-Vis absorption spectroscopy, X-ray diffraction technique, energy-dispersive spectroscopy, and transmission electron microscopy were used to characterize the nanoparticles. Unlike vegetative cells of B. stratosphericus, the spores and the purified DPA were capable of producing nanoparticles from silver nitrate (AgNO3). These biogenic nanoparticles, which were highly toxic against different pathogenic bacteria, showed mixed structures including spherical, triangular, cubic, and hexagonal with the approximate size between 2 and 20 nm in diameter. Our results illustrated the role of dipicolinic acid as a main factor for the synthesis of nanoparticles by the bacterial spores.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Hosseini-Abari A,Emtiazi G,Lee SH,Kim BG,Kim JHdoi
10.1007/s12010-014-1055-3subject
Has Abstractpub_date
2014-09-01 00:00:00pages
270-82issue
1eissn
0273-2289issn
1559-0291journal_volume
174pub_type
杂志文章abstract::Reactive oxygen species (ROS) in plants, arising from various environmental stresses, impair the thiol-contained proteins that are susceptible to irregular oxidative formation of disulfide bonds, which might be alleviated by a relatively specific modifier called protein disulfide isomerase (PDI). From our previous dat...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1585-3
更新日期:2015-05-01 00:00:00
abstract::The production of biodiesel from Scenedesmus algal oil is one of the best alternative forms of liquid fuel production from biomass to petrol diesel. Biodiesel plays a significant role in the carbon sequestration process during cultivation. Scenedesmus algal species was isolated and cultured in a bold basal medium by u...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03149-0
更新日期:2020-04-01 00:00:00
abstract::This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9807-4
更新日期:2012-10-01 00:00:00
abstract::The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescenc...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-016-2242-1
更新日期:2017-02-01 00:00:00
abstract::Adenosine monophosphate-activated protein kinase (AMPK) is viewed as a privileged therapeutic target for several diseases such as cancer, diabetes, inflammation, obesity, etc. In addition, AMPK has entered the limelight of current drug discovery with its evolution as a key metabolic regulator. AMPK also plays a key ro...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s12010-015-1911-9
更新日期:2016-02-01 00:00:00
abstract::This work aimed at investigating both the tolerance and the phytodegradation potential of Erythrina crista-galli L. in petroleum-contaminated soil. It consisted in analyzing E. crista-galli germination, surviving, growth, and development when cultivated at different contaminant concentrations and pollutant degradation...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-009-8531-1
更新日期:2009-04-01 00:00:00
abstract::Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-009-8755-0
更新日期:2010-04-01 00:00:00
abstract::A mesophilic bacterial culture producing a novel thermostable alkaline lipase was isolated from oil rich soil sample and identified as Bacillus subtilis EH 37. The lipase was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography with 17.8-fold purification and 41.9 U/ml specif...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-009-8751-4
更新日期:2010-04-01 00:00:00
abstract::Iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ETS) enzymes which have heme and iron-sulfur centers. In the present study, ETS enzymes, namely, succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), activities ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0273-4
更新日期:2013-08-01 00:00:00
abstract::Strain M(438), deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-pro...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9372-2
更新日期:2011-12-01 00:00:00
abstract::Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scal...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-0761-1
更新日期:2014-03-01 00:00:00
abstract::Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:125:3:159
更新日期:2005-06-01 00:00:00
abstract::Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantificatio...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03179-8
更新日期:2020-05-01 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis heat shock protein 70 (MAPHsp70) is an immunodominant antigen, which can be used as a subunit vaccine against bovine paratuberculosis. In the present study, we evaluated the immunogenic activities of MAPHsp70 expressed by DNA vaccine in chicken and the use of prepared specif...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9648-1
更新日期:2012-05-01 00:00:00
abstract::The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1432-y
更新日期:2015-03-01 00:00:00
abstract::In this work, the catalytic conversion of natural polymer chitosan to 5-HMF under low temperature hydrothermal condition was investigated. The highest 5-HMF yield of 12.1 wt% was obtained under the following conditions: 174 °C reaction temperature, 2.2 % catalyst amount, and 36.9 min reaction time. In the effect of co...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1636-9
更新日期:2015-06-01 00:00:00
abstract::Chemical modification was evaluated to stabilize pig kidney D-amino acid oxidase (pkDAAO), which is required for analytical determination of D-amino acids. Optimization of modification conditions was performed to obtain high recovery yield and stability, and chemical modification at 30 degrees C for 12 h with a highly...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:112:3:123
更新日期:2004-03-01 00:00:00
abstract::The effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on pH and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus Pleurotus ostreatus was determined. The relat...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:110:1:33
更新日期:2003-07-01 00:00:00
abstract::A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-0918-y
更新日期:2014-07-01 00:00:00
abstract::A study was carried out to select the conditions for cultivation of Kluyveromyces marxianus CDBBL 278 in solid-state culture (SSC) using polyurethane foam (PUF) as an inert support. PUF was impregnated with culture media containing lactose (50 g/L) as the carbon and energy source. Evaluation of culture parameters duri...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-008-8268-2
更新日期:2008-12-01 00:00:00
abstract::An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0674-4
更新日期:2014-02-01 00:00:00
abstract::The emergence of nalidixic acid-resistant strains of Salmonella typhimurium remains to be a major public health problem. In particular, the substitution of Asn in place of Asp at the 87 loci in the GyrA of S. typhimurium was experimentally stated for nalidixic acid resistance. However, the data on the possible mechani...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1760-6
更新日期:2015-09-01 00:00:00
abstract::This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was perform...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2763-x
更新日期:2018-11-01 00:00:00
abstract::The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02921507
更新日期:1990-12-01 00:00:00
abstract::Anthracnose is a major disease in Florida hybrid bunch grapes, caused by a fungus viz. Elsinoe ampelina. Florida hybrid bunch grapes are grown in southeastern USA for their superior wine characteristics. However, the effect of anthracnose on grape productivity and wine quality is a major concern to grape growers. Our ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-008-8380-3
更新日期:2009-06-01 00:00:00
abstract::With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02920423
更新日期:1997-04-01 00:00:00
abstract::The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:133:1:1
更新日期:2006-04-01 00:00:00
abstract::Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysi...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-0037-6
更新日期:2013-02-01 00:00:00
abstract::Beta-xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x1), CTAB concentr...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:91-93:1-9:719
更新日期:2001-04-01 00:00:00
abstract::The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate w...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9206-2
更新日期:2011-08-01 00:00:00