Enriched microbial community in bioaugmentation of petroleum-contaminated soil in the presence of wheat straw.


:The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denaturing gradient gel electrophoresis analysis of the amplification of V3 zone of 16S rDNA. Degradation process kinetics was characterized by the degradation ratio of nC17 to nC18. The dehydrogenase activity was also determined during the degradation process. An abrupt change in the microbial community after inoculation was illustrated as well as successive changes in response to degradation of the petroleum contaminants. Seeding with E. cloacae stimulated the growth of other degrading stains such as Pseudomonas sp. and Rhodothermus sp. The application of wheat straw as a representative lignin waste, at 5% (w/w), induced an increase in the total dehydrogenase activity from 0.50 to 0.79, an increase in the microbial content of 130% for bacteria and 84% for fungi, and an increase of the overall degradation ratio from 44% to 56% after 56 days of treatment. The above mentioned results have provided a microbial ecological insight being essential for the design and implementation of bioaugmentation processes.


Wu Z,Dong H,Zou L,Lu D,Liu Z




Has Abstract


2011-08-01 00:00:00












  • Evaluation of textile dye degradation due to the combined action of enzyme horseradish peroxidase and hydrogen peroxide.

    abstract::The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pereira AR,da Costa RS,Yokoyama L,Alhadeff EM,Teixeira LA

    更新日期:2014-12-01 00:00:00

  • Potential use of cutinase in enzymatic scouring of cotton fiber cuticle.

    abstract::The present study characterized the ability of a bacterial cutinase to improve the wettability of raw cotton fabrics by specific hydrolysis of the cutin structure of the cuticle. The effect of cutinase was studied alone and in coreaction with pectin lyase. The changes in both the fabric and the reaction fluid were mea...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Degani O,Gepstein S,Dosoretz CG

    更新日期:2002-07-01 00:00:00

  • Engineering plant alternative oxidase function in mammalian cells: substitution of the motif-like sequence ENV for QDT diminishes catalytic activity of Arum concinnatum AOX1a expressed in HeLa cells.

    abstract::Alternative oxidase (AOX) is a nonproton motive quinol-oxygen oxidoreductase which is a component of the mitochondrial respiratory chain in higher plants. In this study, we have characterized the catalytic activity and regulatory behaviors of Arum concinnatum AOX isoforms, namely AcoAOX1a and AcoAOX1b, and their artif...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kakizaki Y,Ito K

    更新日期:2013-07-01 00:00:00

  • Organic phase synthesis of ethyl oleate using lipases produced by solid-state fermentation.

    abstract::This paper reports a study of the enzymatic esterification of oleic acid and ethanol. The reaction was catalyzed by lipases produced by solid-state fermentation with Rhizopus sp. Olive oil and perlite were used as an inducer and inert support, respectively. Synthesis of ethyl oleate was carried out in a 10-mL batch re...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Martínez-Ruiz A,García HS,Saucedo-Castañeda G,Favela-Torres E

    更新日期:2008-12-01 00:00:00

  • Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation.

    abstract::Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jia J,Chen H,Wu B,Cui F,Fang H,Wang H,Ni Z

    更新日期:2019-01-01 00:00:00

  • Incorportation of Ethyl Esters of EPA and DHA in Soybean Lecithin Using Rhizomucor miehei Lipase: Effect of Additives and Solvent-Free Conditions.

    abstract::The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

    更新日期:2015-06-01 00:00:00

  • Fermentation performance and structure characteristics of xanthan produced by Xanthomonas campestris with a glucose/xylose mixture.

    abstract::The ability of Xanthomonas campestris to convert glucose and xylose to xanthan and the structure of xanthan derived from the glucose/xylose mixture media are important when the lignocelluloses hydrolysate was used in xanthan production. In this paper, the features related to xanthan fermentation in the glucose/xylose ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang Z,Chen H

    更新日期:2010-03-01 00:00:00

  • Enzymatic synthesis of amoxicillin via a one-pot enzymatic hydrolysis and condensation cascade process in the presence of organic co-solvents.

    abstract::A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Wu Q,Chen CX,Du LL,Lin XF

    更新日期:2010-04-01 00:00:00

  • Hydrolysis characteristics of tissue fractions resulting from mechanical separation of corn stover.

    abstract::Corn stover has potential as a resource for both fiber and chemical needs if separation strategies can be developed to deal with its heterogeneity. Relative hydrolysis characteristics were assessed for pith (sclerenchyma and parenchyma) and fiber (collenchyma) tissue fractions derived from mechanical separation of cor...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Bootsma JA,Shanks BH

    更新日期:2005-04-01 00:00:00

  • Effect of temperature and yeast extract on microbial respiration of sediments from a shallow coastal subsurface and vadose zone.

    abstract::As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

    更新日期:1996-04-01 00:00:00

  • Hydrogen and methane production, energy recovery, and organic matter removal from effluents in a two-stage fermentative process.

    abstract::This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Peixoto G,Pantoja-Filho JL,Agnelli JA,Barboza M,Zaiat M

    更新日期:2012-10-01 00:00:00

  • Drug Design for ALK-Positive NSCLC: an Integrated Pharmacophore-Based 3D QSAR and Virtual Screening Strategy.

    abstract::The increasing death rates related to anaplastic lymphoma kinase (ALK)-positive lung cancer culminated in a significant interest in the discovery of novel inhibitors for ALK. In the present research work, pharmacophore-based 3D QSAR modeling and virtual screening strategy have been carried out to address these issues....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: James N,Shanthi V,Ramanathan K

    更新日期:2018-05-01 00:00:00

  • Fourier transform infrared quantitative analysis of sugars and lignin in pretreated softwood solid residues.

    abstract::Hydrolysates were obtained from dilute sulfuric acid pretreatment of whole-tree softwood forest thinnings and softwood sawdust. Mid-infrared (IR) spectra were obtained on sample sets of wet washed hydrolysates, and 45 degrees C vacuum-dried washed hydrolysates, using a Fourier transform infrared (FTIR) spectrophotomet...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Tucker MP,Nguyen QA,Eddy FP,Kadam KL,Gedvilas LM,Webb JD

    更新日期:2001-04-01 00:00:00

  • Bioprocess-Technological Potential of Irradiation-Based Fungal Pretreatment Platform Relevant to Lignocellulolytic Biocascade.

    abstract::Lignocellulose-decaying fungal bioplatforms available are not commercially accessible and are limited to short-term use. In this study, those limitations were overcome by developing a platform using water-soaked rice straw (RS) biodegraded by irradiation-based fungal pretreatment (IBFP). This eco-friendly system incre...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Bak JS

    更新日期:2015-12-01 00:00:00

  • Understanding the Impacts of AFEX™ Pretreatment and Densification on the Fast Pyrolysis of Corn Stover, Prairie Cord Grass, and Switchgrass.

    abstract::Lignocellulosic feedstocks corn stover, prairie cord grass, and switchgrass were subjected to ammonia fiber expansion (AFEX™) pretreatment and densified using extrusion pelleting and ComPAKco densification technique. The effects of AFEX™ pretreatment and densification were studied on the fast pyrolysis product yields....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Sundaram V,Muthukumarappan K,Gent S

    更新日期:2017-03-01 00:00:00

  • Decoding Selection Bias Imparted by Unpaired Cysteines: a Tug of War Between Expression and Affinity.

    abstract::In a recombinant antibody scFv format, the presence of an unpaired cysteine (Cys) is implicated in reduced soluble expression and inefficient presentation in phage display. Compared to other species, antibodies derived from rabbits are more likely to contain this unpaired Cys residue at position 80 (Cys80), when gener...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ayyar BV,Hearty S,O'Kennedy R

    更新日期:2018-07-01 00:00:00

  • Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures.

    abstract::An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde L

    更新日期:2010-03-01 00:00:00

  • Mushroom polysaccharides and lipids synthesized in liquid agitated and static cultures. Part I: screening various mushroom species.

    abstract::The effect of four synthetic media containing glucose (initial concentration 30 g l(-1)) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Diamantopoulou P,Papanikolaou S,Kapoti M,Komaitis M,Aggelis G,Philippoussis A

    更新日期:2012-06-01 00:00:00

  • The constitutive production of pectinase by the CT1 mutant of Penicillium occitainis is modulated by pH.

    abstract::The aim of the present study was to investigate pectinases production by CT1 mutant of Penicillium occitanis on glucose based media. Two main groups of pectinases were followed: lyases (pectin and pectate lyases) and hydrolases (polygalacturonases and polymethylgalacturonases). When cultivated in different liquid medi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Romdhane ZB,Tounsi H,Hadj-Sassi A,Hadj-Taieb N,Gargouri A

    更新日期:2013-01-01 00:00:00

  • Evaluation of a Hypocrea jecorina enzyme preparation for hydrolysis of Tifton 85 bermudagrass.

    abstract::Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ximenes EA,Brandon SK,Doran-Peterson J

    更新日期:2008-03-01 00:00:00

  • Extraction of co-products from biomass: example of thermal degradation of silymarin compounds in subcritical water.

    abstract::In an effort to increase revenues from a given feedstock, valuable co-products could be extracted prior to biochemical or thermochemical conversion with subcritical water. Although subcritical water shows significant promise in replacing organic solvents as an extraction solvent, compound degradation has been observed...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Duan L,Wallace SN,Engelberth A,Lovelady JK,Clausen EC,King JW,Carrier DJ

    更新日期:2009-08-01 00:00:00

  • Continuous production of butanol from starch-based packing peanuts.

    abstract::Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and final starch concentrations were 69.6 and 11.1 g/L, respectively. I...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ezeji TC,Groberg M,Qureshi N,Blaschek HP

    更新日期:2003-04-01 00:00:00

  • Enzymatic biodiesel synthesis in semi-pilot continuous process in near-critical carbon dioxide.

    abstract::A semi-pilot continuous process (SPCP) for enzymatic biodiesel synthesis utilizing near-critical carbon dioxide (NcCO₂) as the reaction medium was developed with the aim of reducing the reaction time and alleviating the catalyst inhibition by methanol. Biodiesel synthesis was evaluated in both lab-scale and semi-pilot...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Lee M,Lee D,Cho J,Kim S,Park C

    更新日期:2013-11-01 00:00:00

  • A novel phosphoramidite method for automated synthesis of oligonucleotides on glass supports for biosensor development.

    abstract::Two protocols for functionalization of glass supports with hexaethylene glycol (HEG)-linked oligonucleotides were developed. The first method (standard amidite protocol) made use of the 2-cyanoethyl-phosphoramidite derivative of 4,4'-dimethoxytrityl-protected HEG. This was first coupled to the support by standard soli...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Sojka B,Piunno PAE,Wust CC,Krull UJ

    更新日期:2000-10-01 00:00:00

  • Comparison of One-Stage Batch and Fed-Batch Enzymatic Hydrolysis of Pretreated Hardwood for the Production of Biosugar.

    abstract::Fed-batch method has shown a great promise in debottlenecking the high-solid enzymatic hydrolysis for the commercialization of cellulosic biosugar conversion for biofuel/biochemical production. To further improve enzymatic hydrolysis efficiency at high solid loading, fed-batch methods of green liquor-pretreated hardwo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: He L,Han Q,Jameel H,Chang HM,Phillips R,Wang Z

    更新日期:2018-04-01 00:00:00

  • Enhancing the enzymatic hydrolysis of corn stover by an integrated wet-milling and alkali pretreatment.

    abstract::An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: He X,Miao Y,Jiang X,Xu Z,Ouyang P

    更新日期:2010-04-01 00:00:00

  • Potential of Ca2+ in Mycobacterium tuberculosis H37Rv Pathogenesis and Survival.

    abstract::The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Sharma S,Meena LS

    更新日期:2017-02-01 00:00:00

  • Isolation and characterization of dimethyl sulfide (DMS)-degrading bacteria from soil and biofilter treating waste gas containing DMS from the laboratory and pulp and paper industry.

    abstract::Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

    更新日期:2012-07-01 00:00:00

  • Diacetyl production mechanism by a strain of Lactococcus lactis spp. lactis bv. diacetylactis. Study of alpha-acetolactic acid extracellular accumulation under anaerobiosis.

    abstract::Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Rondags E,Halliday E,Marc I

    更新日期:1998-02-01 00:00:00

  • Laccase of Coriolus zonatus: isolation, purification, and some physicochemical properties.

    abstract::Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Koroljova OV,Stepanova EV,Gavrilova VP,Biniukov VI,Jaropolov AI,Varfolomeyev SD,Scheller F,Makower A,Otto A

    更新日期:1999-02-01 00:00:00