Enriched microbial community in bioaugmentation of petroleum-contaminated soil in the presence of wheat straw.


:The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denaturing gradient gel electrophoresis analysis of the amplification of V3 zone of 16S rDNA. Degradation process kinetics was characterized by the degradation ratio of nC17 to nC18. The dehydrogenase activity was also determined during the degradation process. An abrupt change in the microbial community after inoculation was illustrated as well as successive changes in response to degradation of the petroleum contaminants. Seeding with E. cloacae stimulated the growth of other degrading stains such as Pseudomonas sp. and Rhodothermus sp. The application of wheat straw as a representative lignin waste, at 5% (w/w), induced an increase in the total dehydrogenase activity from 0.50 to 0.79, an increase in the microbial content of 130% for bacteria and 84% for fungi, and an increase of the overall degradation ratio from 44% to 56% after 56 days of treatment. The above mentioned results have provided a microbial ecological insight being essential for the design and implementation of bioaugmentation processes.


Wu Z,Dong H,Zou L,Lu D,Liu Z




Has Abstract


2011-08-01 00:00:00












  • Effects of oxygen limitation on xylose fermentation, intracellular metabolites, and key enzymes of Neurospora crassa AS3.1602.

    abstract::The effects of oxygen limitation on xylose fermentation of Neurospora crassa AS3.1602 were studied using batch cultures. The maximum yield of ethanol was 0.34 g/g at oxygen transfer rate (OTR) of 8.4 mmol/L.h. The maximum yield of xylitol was 0.33 g/g at OTR of 5.1 mmol/L.h. Oxygen limitation greatly affected mycelia ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang Z,Qu Y,Zhang X,Lin J

    更新日期:2008-03-01 00:00:00

  • Circulating IgSF proteins inhibit adhesion of antibody targeted microspheres to endothelial inflammatory ligands.

    abstract::Proposed methods for detecting circulatory system disease include targeting ultrasound contrast agents to inflammatory markers on vascular endothelial cells. For antibody-based therapies, soluble forms of the targeted adhesion proteins of the immunoglobulin superfamily (IgSF) reduce adhesion yet were left unaccounted ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kerby MB,Urban JC,Mouallem L,Tripathi A

    更新日期:2009-10-01 00:00:00

  • Characterization and performance of immobilized amylase and cellulase.

    abstract::The performance of cellulase and amylase immobilized on siliceous supports was investigated. Enzyme uptake onto the support depended on the enzyme source and immobilization conditions. For amylase, the uptake ranged between 20 and 60%, and for cellulase, 7-10%. Immobilized amylase performance was assessed by batch kin...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Saville BA,Khavkine M,Seetharam G,Marandi B,Zuo YL

    更新日期:2004-04-01 00:00:00

  • Improving stability and activity of cross-linked enzyme aggregates based on polyethylenimine in hydrolysis of fish oil for enrichment of polyunsaturated fatty acids.

    abstract::Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Yan J,Gui X,Wang G,Yan Y

    更新日期:2012-02-01 00:00:00

  • Biosorption of metal ions with Penicillium chrysogenum.

    abstract::Biosorption of metal ions with Penicillium chrysogenum mycelium is described in this article. Alkaline pretreatment was used to remove proteins and nucleic acids from cells, and this treatment increased the adsorption capacities, for Cr3+ from 18.6 mg g(-1) to 27.2 mg g(-1), for Ni2+ from 13.2 mg g(-1) to 19.2 mg g(-1...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Tan T,Cheng P

    更新日期:2003-02-01 00:00:00

  • Methods to enhance tolerances of Chlorella KR-1 to toxic compounds in flue gas.

    abstract::Possible methods to minimize the toxic effects of SOx and NOx on the growth of a highly CO2 tolerant and fast-growing microalga, Chlorella sp. KR-1, were investigated. Maintaining the pH of the culturing media at an adequate value was quite important to enhancing the tolerances of the microalgae to SOx and NOx. Contro...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Lee JN,Lee JS,Shin CS,Park SC,Kim SW

    更新日期:2000-04-01 00:00:00

  • In silico and experimental characterization of chimeric Bacillus thermocatenulatus lipase with the complete conserved pentapeptide of Candida rugosa lipase.

    abstract::Lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. To provide chimeric Bacillus thermocatenulatus lipase (BTL2), the completely conserved pentapeptide (¹¹²Ala-His-Ser-Gln-Gly¹¹⁶) was replaced with similar sequences (²⁰⁷Gly-G...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Hosseini M,Karkhane AA,Yakhchali B,Shamsara M,Aminzadeh S,Morshedi D,Haghbeen K,Torktaz I,Karimi E,Safari Z

    更新日期:2013-02-01 00:00:00

  • Identification and expression of an APETALA2-like gene from Nelumbo nucifera.

    abstract::Arabidopsis transcription factor APETALA2 (AP2) controls multiple aspects of plant growth and development, including seed development, stem cell maintenance, and specification of floral organ identity. Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species, degenerate RT-PCR and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu Z,Gu C,Chen F,Jiang J,Yang Y,Li P,Chen S,Zhang Z

    更新日期:2012-09-01 00:00:00

  • Effect of elicitors and precursors on azadirachtin production in hairy root culture of Azadirachta indica.

    abstract::The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of prec...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Srivastava S,Srivastava AK

    更新日期:2014-02-01 00:00:00

  • Recombination system based on cre alpha complementation and leucine zipper fusions.

    abstract::In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Seidi A,Mie M,Kobatake E

    更新日期:2009-08-01 00:00:00

  • Isolation and screening of microorganisms for R-(+)-limonene and (-)-beta-pinene biotransformation.

    abstract::This work is focused on the biotransformation of R-(+)-limonene and (-)-beta-pinene to bioflavor production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrates. From the isolated microorganisms, 193 were selected in the prescreening using mineral medium for li...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Rottava I,Cortina PF,Grando CE,Colla AR,Martello E,Cansian RL,Toniazzo G,Treichel H,Antunes OA,Oestreicher EG,de Oliveira D

    更新日期:2010-10-01 00:00:00

  • Effects of ELF magnetic field in combination with Iron(III) chloride (FeCl3) on cellular growth and surface morphology of Escherichia coli (E. coli).

    abstract::This study investigated the effects of extremely low frequency (ELF) magnetic field with/without iron(III) chloride (FeCl3) on bacterial growth and morphology. The ELF exposures were carried out using a pair of Helmholtz coil-based ELF exposure system which was designed to generate 50 Hz sinusoidal magnetic field. The...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Esmekaya MA,Acar SI,Kıran F,Canseven AG,Osmanagaoglu O,Seyhan N

    更新日期:2013-04-01 00:00:00

  • Chitosan-modified carbon nanotubes-based platform for low-density lipoprotein detection.

    abstract::We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

    更新日期:2014-10-01 00:00:00

  • Sphaerotilus natans isolated from activated sludge and its production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate).

    abstract::Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu K,Chua H,Lo WH,Lawford H,Yu PH

    更新日期:2002-04-01 00:00:00

  • Continuous Production of Isomalto-oligosaccharides by Thermo-inactivated Cells of Aspergillus niger J2 with Coarse Perlite as an Immobilizing Material.

    abstract::The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixtur...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Huang Z,Li Z,Su Y,Zhu Y,Zeng W,Chen G,Liang Z

    更新日期:2018-08-01 00:00:00

  • Molecular Characterization of Xylobiose- and Xylopentaose-Producing β-1,4-Endoxylanase SCO5931 from Streptomyces coelicolor A3(2).

    abstract::Streptomyces coelicolor A3(2) sco5931 gene was predicted to encode a putative xylanase A, a 477 amino acid protein belonging to glycoside hydrolase family 10. The entire sco5931 coding region was cloned and overexpressed in Streptomyces lividans TK24. Mature SCO5931 protein comprising 436 amino acids (47 kDa) was puri...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Enkhbaatar B,Lee CR,Hong YS,Hong SK

    更新日期:2016-09-01 00:00:00

  • Ornithine carbamoyltransferase unfolding states in the presence of urea and guanidine hydrochloride.

    abstract::Ornithine carbamoyltransferase folding/unfolding is a complex and not completely understood process. Our experimental results suggest that ornithine carbamoyltransferase interacts in a completely different way with urea and guanidine hydrochloride. In fact, we noticed that, increasing concentration from 0.0 to 8.0 M o...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Barreca D,Laganà G,Ficarra S,Tellone E,Leuzzi U,Galtieri A,Bellocco E

    更新日期:2014-01-01 00:00:00

  • A trypsin inhibitor from rambutan seeds with antitumor, anti-HIV-1 reverse transcriptase, and nitric oxide-inducing properties.

    abstract::Nephelium lappaceum L., commonly known as "rambutan," is a typical tropical tree and is well known for its juicy and sweet fruit which has an exotic flavor. Chemical studies on rambutan have led to the identification of various components such as monoterpene lactones and volatile compounds. Here, a 22.5-kDa trypsin in...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Fang EF,Ng TB

    更新日期:2015-04-01 00:00:00

  • Influence of Altered NADH Metabolic Pathway on the Respiratory-deficient Mutant of Rhizopus oryzae and its L-lactate Production.

    abstract::Respiratory-deficient mutants of Rhizopus oryzae (R. oryzae) AS 3.3461 were acquired by ultraviolet (UV) irradiation to investigate changes in intracellular NADH metabolic pathway and its influence on the fermentation characteristics of the strain. Compared with R. oryzae AS 3.3461, the intracellular ATP level of the ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Shu C,Guo C,Luo S,Jiang S,Zheng Z

    更新日期:2015-08-01 00:00:00

  • Polykaryon formation using a swollen conidium of Trichoderma reesei.

    abstract::The cellulolytic fungus, Trichoderma has oval and mononucleate conidia. When these conidia are incubated in a liquid medium, they begin to swell and their shape becomes spherical followed by an increase in inner space. In such swollen conidia, it is possible to produce a larger autopolyploid nucleus using a mitotic ar...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Toyama H,Yano M,Hotta T

    更新日期:2004-04-01 00:00:00

  • Dependency of cellular decision making in physiology and influence of preceding growth conditions.

    abstract::Events from the past growth conditions influence the course of physiology, and it gets reflected in the present cell behaviour. During this process, cells acquire a metabolic option which carries signature of the past, and it dictates the performance in the present situation. Study uses Escherichia coli as a sample or...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Puranik S,Purohit HJ

    更新日期:2014-11-01 00:00:00

  • Sensitive immunodetection through impedance measurements onto gold functionalized electrodes.

    abstract::This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

    更新日期:2000-11-01 00:00:00

  • Development of a Loop-Mediated Isothermal Amplification Assay for Rapid and Specific Identification of ACT Producing Alternaria alternata, the Agent of Brown Spot Disease in Tangerine.

    abstract::Rapid, accurate, and easy identification of pathogenic agents has always been important in medicine, veterinary, and agriculture. The brown spot infection is among the most common diseases in tangerine caused by Alternaria alternata. Due to the existence of seven various pathotypes of A. alternata species, it is chall...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Moghimi H,Moradi A,Hamedi J,Basiri M

    更新日期:2016-03-01 00:00:00

  • New potential biocatalysts by laccase immobilization in PVA Cryogel type carrier.

    abstract::Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Stanescu MD,Fogorasi M,Shaskolskiy BL,Gavrilas S,Lozinsky VI

    更新日期:2010-04-01 00:00:00

  • Purification of a PHA-like chitin-binding protein from Acacia farnesiana seeds: a time-dependent oligomerization protein.

    abstract::A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) follow...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Santi-Gadelha T,Rocha BA,Oliveira CC,Aragão KS,Marinho ES,Gadelha CA,Toyama MH,Pinto VP,Nagano CS,Delatorre P,Martins JL,Galvani FR,Sampaio AH,Debray H,Cavada BS

    更新日期:2008-07-01 00:00:00

  • BTX removal from polluted water through bioleaching processes.

    abstract::In this study, benzene, toluene, and xylenes (BTX) removal from contaminated water by physical, chemical, and biological processes was studied. Results showed that air sparging in polluted water can reduce monoaromatic compounds from 140,000 to about 5 microg/l in only 1 h process with a gassing rate of 0.33 VVM. This...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Farhadian M,Duchez D,Vachelard C,Larroche C

    更新日期:2008-12-01 00:00:00

  • Relationship between coffee husk caffeine degradation and respiration of Aspergillus sp. LPBx in solid-state fermentation.

    abstract::Studies were carried out in a packed-bed column fermentor using coffee husk as substrate in order to verify a relationship between caffeine degradation and the respiration of Aspergillus sp. LPBx. Fermentation conditions were optimized by using factorial design experiments. The kinetic study showed that the caffeine d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Brand D,Pandey A,Rodriguez-Leon JA,Roussos S,Brand I,Soccol CR

    更新日期:2002-07-01 00:00:00

  • The effect of composition of parenteral solution on the thermal resistance of Bacillus stearothermophilus and Bacillus subtilis spores.

    abstract::Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Penna TC,Marques M,Machoshvili IA,Ishii M

    更新日期:2002-04-01 00:00:00

  • Production of insoluble exopolysaccharide of Agrobacterium sp. (ATCC 31749 and IFO 13140).

    abstract::Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Portilho M,Matioli G,Zanin GM,de Moraes FF,Scamparini AR

    更新日期:2006-03-01 00:00:00

  • Effect of VIB Gene on Cellulase Production of Trichoderma orientalis EU7-22.

    abstract::The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Han J,Xue Y,Li M,Li Y,Liu J,Gan L,Long M

    更新日期:2020-08-01 00:00:00