Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae.

Abstract:

:A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively, whereas transcription and translation in P stipitis were inducible. S. cerevisiae carrying the xylose reductase gene could not, however, grow on xylose medium, and could not produce ethanol from xylose. Since xylose uptake and accumulation of xylitol by S. cerevisiae were observed, the conversion of xylitol to xylulose seemed to be limited.

authors

Takuma S,Nakashima N,Tantirungkij M,Kinoshita S,Okada H,Seki T,Yoshida T

doi

10.1007/BF02922612

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

327-40

eissn

0273-2289

issn

1559-0291

journal_volume

28-29

pub_type

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