Improved synthesis of (S)-1-phenyl-2-propanol in high concentration with coupled whole cells of Rhodococcus erythropolis and Bacillus subtilis on preparative scale.

Abstract:

:Bioreduction of 1-phenyl-2-propanone to prepare (S)-1-phenyl-2-propanol, a useful pharmaceutical intermediate, was performed with growing cells of Rhodococcus erythropolis JX-021, giving 14 mM (1.9 g/L) product in 99% e.e. at 5 h in the catalysis of 15 mM substrate. The reduction stopped afterwards due to strong inhibition of substrate and formed product, a problem that is often encountered in biotransformation. While the substrate inhibition was solved by stepwise feeding, product inhibition was tackled by different methods: repeated removal of the product by centrifugation, by absorption with Amberlite XAD-7 resin, and by the use of dodecanol as the second phase gave the final product in 58, 68, and 61 mM in the catalysis of 80 mM substrate, respectively. The inhibition was caused by the partial permeabilization of cell membrane of R. erythropolis JX-021, and addition of NADPH or glucose 6-phosphate to such cell culture retained the reduction activity. Therefore, higher productivity in the reduction of 1 with resting cells of R. erythropolis JX-021 was achieved through cofactor regeneration and recycling by the addition of glucose and permeabilized cells of Bacillus subtilis BGSC 1A1 containing a glucose dehydrogenase, giving the product in 62 mM without addition of cofactor and 78 mM with the addition of 0.01 mM NADP(+) in the catalysis of 120 mM substrate. The product e.e. retained 99% during the process which showed industrial possibility.

authors

Jin JZ,Li H,Zhang J

doi

10.1007/s12010-010-8983-3

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

2075-86

issue

7

eissn

0273-2289

issn

1559-0291

journal_volume

162

pub_type

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