An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening.

abstract：:Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation. Presence of silic...

journal_title：Applied biochemistry and biotechnology

authors： Krishnamoorthy S,Iyer NR,Murthy AR

更新日期：2015-02-01 00:00:00

abstract：:The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect ...

journal_title：Applied biochemistry and biotechnology

authors： Richardson PJ,Luzio JP

更新日期：1986-10-01 00:00:00

abstract：:The batch culture of a newly isolated strain of a green microalga, Chlorella sorokiniana, was carried out using a conical helical tubular photobioreactor. The isolate was capable of good growth at 40 degrees C under an airstream enriched with 10% CO2. The maximum photosynthetic productivity was 34.4 g of dry biomass/(...

journal_title：Applied biochemistry and biotechnology

authors： Morita M,Watanabe Y,Saiki H

更新日期：2000-06-01 00:00:00

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:The potential market for lactic acid as the feedstock for biodegradable polymers, oxygenated chemicals, and specialty chemicals is significant. L-lactic acid is often the desired enantiomer for such applications. However, stereospecific lactobacilli do not metabolize starch efficiently. In this work, Argonne researche...

journal_title：Applied biochemistry and biotechnology

authors： Tsai SP,Moon SH

更新日期：1998-04-01 00:00:00

abstract：:The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

journal_title：Applied biochemistry and biotechnology

authors： Sharma S,Meena LS

更新日期：2017-02-01 00:00:00

abstract：:This study presents the production of bacterial cellulose (BC) by a bacterium isolated from a rotten fruit and its process optimization. Here, isolation and screening of potent cellulose producers were carried out from different natural sources, viz., soil, rotten fruits, and vegetables and vinegar. A total of 200 bac...

journal_title：Applied biochemistry and biotechnology

authors： Jahan F,Kumar V,Rawat G,Saxena RK

更新日期：2012-07-01 00:00:00

abstract：:As a part of a natural biological N-cycle, nitrification is one of the steps included in the conception of artificial ecosystems designed for extraterrestrial life support systems (LSS) such as Micro-Ecological Life Support System Alternative (MELiSSA) project, which is the LSS project of the European Space Agency. Ni...

journal_title：Applied biochemistry and biotechnology

authors： Farges B,Poughon L,Roriz D,Creuly C,Dussap CG,Lasseur C

更新日期：2012-07-01 00:00:00

abstract：:A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...

journal_title：Applied biochemistry and biotechnology

authors： Mohanraj S,Kodhaiyolii S,Rengasamy M,Pugalenthi V

更新日期：2014-05-01 00:00:00

abstract：:Solid-state fermentation using the microfungus Penicillium brevicompactum for the production of mycophenolic acid is reported in this paper. Of the initial substrates tested (whole wheat, cracked wheat, long grain Basmati rice, and short grain Parmal rice), Parmal rice proved to be the best. Under initial conditions, ...

journal_title：Applied biochemistry and biotechnology

authors： Patel G,Patil MD,Soni S,Chisti Y,Banerjee UC

更新日期：2017-05-01 00:00:00

abstract：:An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...

journal_title：Applied biochemistry and biotechnology

authors： Cheruvathur MK,Thomas TD

更新日期：2014-02-01 00:00:00

abstract：:Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

journal_title：Applied biochemistry and biotechnology

authors： Yan J,Gui X,Wang G,Yan Y

更新日期：2012-02-01 00:00:00

abstract：:Arabidopsis transcription factor APETALA2 (AP2) controls multiple aspects of plant growth and development, including seed development, stem cell maintenance, and specification of floral organ identity. Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species, degenerate RT-PCR and...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Jiang J,Yang Y,Li P,Chen S,Zhang Z

更新日期：2012-09-01 00:00:00

abstract：:The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal i...

journal_title：Applied biochemistry and biotechnology

authors： Mulay YR,Deopurkar RL

更新日期：2018-02-01 00:00:00

abstract：:The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran P,Narayanan GK,Gandhi S,Sethuraman S,Krishnan UM

更新日期：2015-03-01 00:00:00

abstract：:Glutathione (γ-glutamyl-L-cysteinylglycine, GSH) is a pharmaceutical compound often used in food additives and the cosmetics industry. GSH can be produced biologically from L-glutamic acid, L-cysteine, and glycine through an enzymatic process traditionally involving two sequential adenosine triphosphate (ATP)-dependen...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Tao R,Shen Z,Sun L,Zhu F,Yang S

更新日期：2016-12-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:Alpha-lactalbumin hydrolysate is of significant interest, due to its potential application as a source of bioactive peptides in nutraceutical and pharmaceutical domains. This study was focused on the cholecystokinin (CCK) family compounds which are small peptides involved in the satiety control. The action of chymotry...

journal_title：Applied biochemistry and biotechnology

authors： Catiau L,Delval-Dubois V,Guillochon D,Nedjar-Arroume N

更新日期：2011-11-01 00:00:00

abstract：:Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnost...

journal_title：Applied biochemistry and biotechnology

authors： Wai-Hoe L,Wing-Seng L,Ismail Z,Lay-Harn G

更新日期：2009-10-01 00:00:00

abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

更新日期：2016-07-01 00:00:00

abstract：:Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified org...

journal_title：Applied biochemistry and biotechnology

authors： Elsanhoty RM,Al-Turki AI,Ramadan MF

更新日期：2013-10-01 00:00:00

abstract：:Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a ...

journal_title：Applied biochemistry and biotechnology

authors： Lee SH,Lee JE,Kim Y,Lee SY

更新日期：2016-01-01 00:00:00

abstract：:The autoimmune repertoire is well known from previous studies to be capable of producing catalytic antibodies directed to self-antigens. In the present study, we explored the ability of 26 monoclonal light chains (L chains) from multiple myeloma patients to cleave radiolabeled gp120, a foreign protein. One L chain wit...

journal_title：Applied biochemistry and biotechnology

authors： Paul S,Kalaga RS,Gololobov G,Brenneman D

更新日期：2000-01-01 00:00:00

abstract：:Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated...

journal_title：Applied biochemistry and biotechnology

authors： Hu C,Choy SY,Giannis A

更新日期：2018-05-01 00:00:00

abstract：:A spectrophotometric method of measuring oxygenase activity in cell extracts or in zymograms was developed. It is an easy and cheap method that allows spectrophotometric measurement of activity by a colored reaction and reveals activity bands in a polyacrylamide gel electrophoresis (PAGE) gel as brown bands. To prove ...

journal_title：Applied biochemistry and biotechnology

authors： Zazueta-Sandoval R,Novoa VZ,Jiménez HS,Ortiz RC

更新日期：2003-04-01 00:00:00

abstract：:Sequencing batch reactors (SBRs) including aerobic SBRs and anaerobic SBRs (ASBRs) are partially emptied batch reactors that are widely used as bioprocesses in pollution control. We present dilution and biochemical materials balance modeling equations and simulation results for the partially emptied batch reactors, es...

journal_title：Applied biochemistry and biotechnology

authors： Ndon UJ,Zhao JC,Siguan ML

更新日期：2007-01-01 00:00:00

abstract：:Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of usi...

journal_title：Applied biochemistry and biotechnology

authors： Niyonzima FN,More SS

更新日期：2014-10-01 00:00:00

abstract：:The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Vincenti S,Mariani M,Brunini-Bronzini de Caraffa V,Gambotti C,Desjobert JM,Muselli A,Costa J,Tomi F,Berti L,Maury J

更新日期：2017-07-01 00:00:00

abstract：:Since the two reports published in 1986 by the laboratories of R. Lerner and P. G. Schultz, it has been clearly established that antibodies may be induced to act as catalysts in numerous chemical reactions. In all cases, catalytic antibodies were elicited using a substrate-based approach. In the present article, we pr...

journal_title：Applied biochemistry and biotechnology

authors： Friboulet A,Izadyar L,Avalle B,Roseto A,Thomas D

更新日期：1994-05-01 00:00:00

abstract：:We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polya...

journal_title：Applied biochemistry and biotechnology

authors： Okamura Y,Takeyama H,Matsunaga T

更新日期：2000-04-01 00:00:00