Enzymatic Production of Glutathione by Bifunctional γ-Glutamylcysteine Synthetase/Glutathione Synthetase Coupled with In Vitro Acetate Kinase-Based ATP Generation.

abstract：:Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to for...

journal_title：Applied biochemistry and biotechnology

authors： Bhuvana M,Dharuman V

更新日期：2014-10-01 00:00:00

abstract：:An Aspergillus terreus, isolated from oil contaminated soil, could degrade a wide range of petroleum hydrocarbons including the immediate oxidation products of hydrocarbons, like alkanols and alkanals. Among all the linear chain carbon substrates, highest growth of 39.1 + or - 3.8 g l(-1) (wet weight) was observed whe...

journal_title：Applied biochemistry and biotechnology

authors： Kumar AK,Vatsyayan P,Goswami P

更新日期：2010-03-01 00:00:00

abstract：:An economic evaluation model was developed in the Laboratory of Agricultural Structures (LAS) of the Agricultural University of Athens-the Modified Basic Economic Evaluation Model (MBEEM). This model is an improved version of the original Basic Economic Evaluation Model, available in LAS, and it is used to assess the ...

journal_title：Applied biochemistry and biotechnology

authors： Georgakakis D,Christopoulou N,Chatziathanassiou A,Venetis T

更新日期：2003-04-01 00:00:00

abstract：:A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

journal_title：Applied biochemistry and biotechnology

authors： Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

更新日期：2013-05-01 00:00:00

abstract：:This study investigated the effects of flask-to-liquid volume ratio on the growth of Panax ginseng hairy root, transformed by Agrobacterium rhizogenes, in flask cultures and compared the characteristics of various bioreactors for scale-up. The flask-to-liquid volume ratio was optimum at 1.5 mL of air/mL of medium in f...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Hwang B,Woo JC

更新日期：2003-04-01 00:00:00

abstract：:The ability of poly(ethylene glycol) (PEG) to protect enzymatic peroxidase activity was determined for horseradish peroxidase (HRP), versatile peroxidase (VP), commercial Coprinus peroxidase (BP), and chloroperoxidase (CPO). The operational stability measured as the total turnover number was determined for the four pe...

journal_title：Applied biochemistry and biotechnology

authors： Juarez-Moreno K,Ayala M,Vazquez-Duhalt R

更新日期：2015-11-01 00:00:00

abstract：:Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in resp...

journal_title：Applied biochemistry and biotechnology

authors： Fei YY,Bhat JA,Gai JY,Zhao TJ

更新日期：2020-08-01 00:00:00

abstract：:Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, star...

journal_title：Applied biochemistry and biotechnology

authors： Nampoothiri KM,Tomes GJ,Roopesh K,Szakacs G,Nagy V,Soccol CR,Pandey A

更新日期：2004-07-01 00:00:00

abstract：:Oil palm empty fruit bunch (EFB) is abundantly available in Malaysia and it is a potential source of xylose for the production of high-value added products. This study aimed to optimize the hydrolysis of EFB using dilute sulfuric acid (H2SO4) and phosphoric acid (H3PO4) via response surface methodology for maximum xyl...

journal_title：Applied biochemistry and biotechnology

authors： Tan HT,Dykes GA,Wu TY,Siow LF

更新日期：2013-08-01 00:00:00

abstract：:Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of...

journal_title：Applied biochemistry and biotechnology

authors： Sukhumsirichart W,Deesukon W,Kawakami T,Matsumoto S,Seesom W,Sakamoto T

更新日期：2014-01-01 00:00:00

abstract：:Androstenedione (AD) is a steroid intermediate used in the pharmaceutical industry for the production of several important anabolic drugs. An important route for producing AD is by the microbial transformation of sterols. Microbes capable of efficiently transforming sterols to AD are few and newer strains need to be i...

journal_title：Applied biochemistry and biotechnology

authors： Malaviya A,Gomes J

更新日期：2009-08-01 00:00:00

abstract：:The epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties. It is believed that EMT is associated with initiation and completion of the invasion-metastasis cascade. In this study, an economic approach wa...

journal_title：Applied biochemistry and biotechnology

authors： Li Y,Gao A,Yu L

更新日期：2016-01-01 00:00:00

abstract：:Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on ...

journal_title：Applied biochemistry and biotechnology

authors： Yusof MFH,Hashim SNM,Zahari W,Chandra H,Noordin KBAA,Kannan TP,Hamid SSA,Mokhtar KI,Azlina A

更新日期：2020-05-01 00:00:00

abstract：:Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then appl...

journal_title：Applied biochemistry and biotechnology

authors： Baloch KA,Upaichit A,Cheirsilp B

更新日期：2021-01-01 00:00:00

abstract：:We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

journal_title：Applied biochemistry and biotechnology

authors： Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

更新日期：2014-10-01 00:00:00

abstract：:Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...

journal_title：Applied biochemistry and biotechnology

authors： Karadas O,Mese G,Ozcivici E

更新日期：2019-06-01 00:00:00

abstract：:Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the format...

journal_title：Applied biochemistry and biotechnology

authors： Sørensen HP,Madsen LS,Petersen J,Andersen JT,Hansen AM,Beck HC

更新日期：2010-03-01 00:00:00

abstract：:Cell-penetrating peptides are molecules with the ability to cross membranes and enter cells. Attention has been put on these peptides as a tool for drug delivery research, as they are able to serve as delivery vectors for large molecules. Intracellular delivery of bioactive peptides is a very promising research area f...

journal_title：Applied biochemistry and biotechnology

authors： Rodríguez V,Lascani J,Asenjo JA,Andrews BA

更新日期：2015-03-01 00:00:00

abstract：:Microalgae are photosynthetic microorganisms that produce several bioactive molecules that have received considerable attention in scientific and industrial communities. Today, many plant biostimulants including seaweed extracts and polysaccharides are used in agriculture. However, microalgae have not been largely exp...

journal_title：Applied biochemistry and biotechnology

authors： Farid R,Mutale-Joan C,Redouane B,Mernissi Najib EL,Abderahime A,Laila S,Arroussi Hicham EL

更新日期：2019-05-01 00:00:00

abstract：:A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

journal_title：Applied biochemistry and biotechnology

authors： Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

更新日期：2008-04-01 00:00:00

abstract：:The effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and c...

journal_title：Applied biochemistry and biotechnology

authors： Andrade MM,Barbosa AM,Bofinger MR,Dekker RF,Messias JM,Guedes CL,Zaminelli T,de Oliveira BH,de Lima VM,Dall'antonia LH

更新日期：2013-08-01 00:00:00

abstract：:Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, th...

journal_title：Applied biochemistry and biotechnology

authors： Zakaria II,Rahman RN,Salleh AB,Basri M

更新日期：2011-09-01 00:00:00

abstract：:This research describes the development of a multi-analyte lateral-flow immunoassay intended for the simultaneous detection of three anti-protozoan drugs (coccidiostats). These drugs, namely, halofuginone, toltrazuril and diclazuril, are used in the treatment of Eimeria spp. infections in cattle, pigs, chickens and tu...

journal_title：Applied biochemistry and biotechnology

authors： Fitzgerald J,Leonard P,Danaher M,O'Kennedy R

更新日期：2015-05-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:Batch pressure vessels commonly used for hydrothermal liquefaction have typical heating times in the range of 30 to 60 min. Thermodynamically, the complex set of reactions are path dependent, so that the heating rate can possibly affect yields and the composition of the resultant liquid products. It is postulated that...

journal_title：Applied biochemistry and biotechnology

authors： Zhang B,von Keitz M,Valentas K

更新日期：2008-03-01 00:00:00

abstract：:Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Four...

journal_title：Applied biochemistry and biotechnology

authors： Singh S,Singh AN,Verma A,Dubey VK

更新日期：2013-12-01 00:00:00

abstract：:Aldehyde dehydrogenase (E.C. 1.2.1.x) can catalyze detoxification of acetaldehydes. A novel acetaldehyde dehydrogenase (istALDH) from the non-Saccharomyces yeast Issatchenkia terricola strain XJ-2 has been previously characterized. In this work, Lactococcus lactis with the NIsin Controlled Expression (NICE) System was...

journal_title：Applied biochemistry and biotechnology

authors： Lyu Y,LaPointe G,Zhong L,Lu J,Zhang C,Lu Z

更新日期：2018-02-01 00:00:00

abstract：:Palm kernel press cake (PKC) is a residue from palm oil extraction presently only used as a low protein feed supplement. PKC contains 50% fermentable hexose sugars present in the form of glucan and mainly galactomannan. This makes PKC an interesting feedstock for processing into bioethanol or in other biorefinery proc...

journal_title：Applied biochemistry and biotechnology

authors： Jørgensen H,Sanadi AR,Felby C,Lange NE,Fischer M,Ernst S

更新日期：2010-05-01 00:00:00

abstract：:Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA...

journal_title：Applied biochemistry and biotechnology

authors： Qiao G,Wen XP,Zhang T

更新日期：2015-12-01 00:00:00

abstract：:In the present investigation, thermophilic fungus Rhizomucor pusillus was used to study biotransformation of antihelmintic drug albendazole to produce its active metabolite, albendazole sulfoxide and novel metabolites of commercial interest. A two-stage fermentation procedure was followed for biotransformation of albe...

journal_title：Applied biochemistry and biotechnology

authors： Prasad GS,Girisham S,Reddy SM

更新日期：2011-11-01 00:00:00