Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).


:Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA ends (RACE) method. It was designated CcLHCB1 and encoded a 262 amino acid protein. The calculated molecular weight of the CcLHCB1 protein was 27.89 kDa, and the theoretical isoelectric point was 5.29. Homology search and sequence multi-alignment demonstrated that the CcLHCB1 protein sequence shared a high identity with LHCB from other plants. Bioinformatics analysis revealed that CcLHCB1 was a hydrophobic protein with three transmembrane domains. By fluorescent quantitative real-time polymerase chain reaction (PCR), CcLHCB1 mRNA transcripts were detectable in different tissues (leaf, stem, and root), with the highest level found in the leaf. The expression of CcLHCB1 mRNA in the leaves was up-regulated by drought stimulation and AM inoculation. Our results provide the basis for a better understanding of the molecular organization of LCHB and might be useful for understanding the interaction between plants and microbes in the future.


Qiao G,Wen XP,Zhang T




Has Abstract


2015-12-01 00:00:00














  • Sequential anaerobic/aerobic treatment of dye-containing wastewaters: colour and COD removals, and ecotoxicity tests.

    abstract::Colour and COD removals of the azo dyes Congo Red (CR) and Reactive Black 5 (RB5) were individually evaluated in a sequential anaerobic/aerobic treatment system. Additionally, dye toxicity was assessed by using acute ecotoxicity tests with Daphnia magna as the indicator-organism. The anaerobic reactor was operated at ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Silva ME,Firmino PI,Sousa MR,Santos AB

    更新日期:2012-02-01 00:00:00

  • Refolding of a staphylokinase variant y1-Sak by reverse dilution.

    abstract::To develop more potent thrombolytic agents with fibrinolytic and antiplatelet aggregation activity, staphylokinase (Sak) variant Y1-Sak, a recombinant mutant of the Staphylococcus aureus protein Sak, was constructed. Y1-Sak formed an insoluble inclusion body when overexpressed in Escherichia coli strain JF1125. To obt...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: He J,Wang G,Xu R,Feng J,Wang J,Su H,Song H

    更新日期:2008-10-01 00:00:00

  • Expression and characterization of a new thermostable esterase from Clostridium thermocellum.

    abstract::The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni(2+) affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang T,Chen H,Ni Z,Tian R,Jia J,Chen Z,Yang S

    更新日期:2015-12-01 00:00:00

  • Atmospheric pressure plasma pretreatment of sugarcane bagasse: the influence of moisture in the ozonation process.

    abstract::Sugarcane bagasse samples were pretreated with ozone via atmospheric O2 pressure plasma. A delignification efficiency of approximately 80 % was observed within 6 h of treatment. Some hemicelluloses were removed, and the cellulose was not affected by ozonolysis. The quantity of moisture in the bagasse had a large influ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Souza-Corrêa JA,Oliveira C,Wolf LD,Nascimento VM,Rocha GJ,Amorim J

    更新日期:2013-09-01 00:00:00

  • Fermentation performance and structure characteristics of xanthan produced by Xanthomonas campestris with a glucose/xylose mixture.

    abstract::The ability of Xanthomonas campestris to convert glucose and xylose to xanthan and the structure of xanthan derived from the glucose/xylose mixture media are important when the lignocelluloses hydrolysate was used in xanthan production. In this paper, the features related to xanthan fermentation in the glucose/xylose ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang Z,Chen H

    更新日期:2010-03-01 00:00:00

  • Effects of ELF magnetic field in combination with Iron(III) chloride (FeCl3) on cellular growth and surface morphology of Escherichia coli (E. coli).

    abstract::This study investigated the effects of extremely low frequency (ELF) magnetic field with/without iron(III) chloride (FeCl3) on bacterial growth and morphology. The ELF exposures were carried out using a pair of Helmholtz coil-based ELF exposure system which was designed to generate 50 Hz sinusoidal magnetic field. The...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Esmekaya MA,Acar SI,Kıran F,Canseven AG,Osmanagaoglu O,Seyhan N

    更新日期:2013-04-01 00:00:00

  • Biodegradation of chlorinated aliphatic hydrocarbon mixtures in a single-pass packed-bed reactor.

    abstract::Aliphatic chlorinated compounds, such as trichloroethylene (TCE) and tetrachloroethylene (PCE), are major contaminants of ground water. A single-pass packed-bed bioreactor was utilized to study the biodegradation of organic waste mixtures consisting of PCE, TCE, and other short-chain chlorinated organics. The bioreact...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Lackey LW,Phelps TJ,Bienkowski PR,White DC

    更新日期:1993-04-01 00:00:00

  • Fusel oil as precursor for aroma generation by biotransformation using lipase: scientific note.

    abstract::The feasibility of using mixtures of C4 and C5 chain-length aliphatic alcohols from fusel oil as the bulk organic media for lipase-mediated synthesis of laurate esters was assessed. Reaction mixtures consisted of lauric acid, lipase, solvent (if added), and appropriate amount of fusel oil (previously dehydrated with i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: De Castro HF,Moriya RY,Oliveira PC,Soares CM

    更新日期:1999-04-01 00:00:00

  • Enhanced Enzymatic Hydrolysis of Sorghum Stalk by Supercritical Carbon Dioxide and Ultrasonic Pretreatment.

    abstract::Sorghum was pretreated by sole ultrasound or supercritical carbon dioxide (scCO2), as well as the method combining both to intensify enzymatic hydrolysis. The effect of the time (1-5 h) and temperature (30-70 °C) on ultrasonic pretreatment was investigated, and the best condition was determined as 5 h and 50 °C with t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang Q,Zhao M,Xu Q,Ren H,Yin J

    更新日期:2019-05-01 00:00:00

  • Composite Electrospun Scaffold Derived from Recombinant Fibroin of Weaver Ant (Oecophylla smaragdina) as Cell-Substratum.

    abstract::Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomateri...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Khamhaengpol A,Siri S

    更新日期:2017-09-01 00:00:00

  • Distribution of hydrocarbon-degrading bacteria in the soil environment and their contribution to bioremediation.

    abstract::A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

    更新日期:2013-05-01 00:00:00

  • Domain-Specific Monoclonal Antibodies Against Human Rev-erbβ.

    abstract::The nuclear receptor Rev-erbβ is a potent transcriptional factor whose functional study has been limited by the lack of suitable antibodies against it. To better understand Rev-erbβ's biological roles, we generated five hybridoma cell lines secreting antibodies against human Rev-erbβ in mice immunized with the purifie...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Chen F,Li Y,Zhao J,Mao Q,Xia H

    更新日期:2017-07-01 00:00:00

  • Modification of lysine residues of horseradish peroxidase and its effect on stability and structure of the enzyme.

    abstract::Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Hassani L,Nourozi R

    更新日期:2014-04-01 00:00:00

  • Anaerobic Co-Digestion of Vegetable and Fruit Market Waste in LBR + CSTR Two-Stage Process for Waste Reduction and Biogas Production.

    abstract::Vegetable and fruit waste (VFW) is becoming a heavy burden of municipal waste disposal because of its huge amount, but it is a potentially valuable resource that can be developed into high value products such as methane. Conventional anaerobic digestion processes are not suitable for solving the problem of easy acidif...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu WY,Liao B

    更新日期:2019-05-01 00:00:00

  • The Production of High Purity Phycocyanin by Spirulina platensis Using Light-Emitting Diodes Based Two-Stage Cultivation.

    abstract::Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Lee SH,Lee JE,Kim Y,Lee SY

    更新日期:2016-01-01 00:00:00

  • Production of exocellular polysaccharide by Azotobacter chroococcum.

    abstract::Environmental conditions affect the production of extracellular polysaccharide by Azotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored expopolysaccharide production, which reache...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: De la Vega MG,Cejudo FJ,Paneque A

    更新日期:1991-09-01 00:00:00

  • RNA interference--a silent but an efficient therapeutic tool.

    abstract::RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in com...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Ramachandran PV,Ignacimuthu S

    更新日期:2013-03-01 00:00:00

  • Production and characterization of a milk-clotting protease produced in submerged fermentation by the thermophilic fungus Thermomucor indicae-seudaticae N31.

    abstract::Proteases are some of the most important industrial enzymes, and one of their main applications is for the production of cheese in the dairy industry. Due to a shortage of animal rennet, microbial coagulant proteases are being sought. In this work, the production of microbial rennet from Thermomucor indicae-seudaticae...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Silva BL,Geraldes FM,Murari CS,Gomes E,Da-Silva R

    更新日期:2014-02-01 00:00:00

  • Effector-assisted refolding of recombinant tissue-plasminogen activator produced in Escherichia coli.

    abstract::Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Grunfeld H,Patel A,Shatzman A,Nishikawa AH

    更新日期:1992-05-01 00:00:00

  • Compositional changes in sugarcane bagasse on low temperature, long-term diluted ammonia treatment.

    abstract::Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kim M,Aita G,Day DF

    更新日期:2010-05-01 00:00:00

  • Isolation and Characterization of Bacteria That Use Furans as the Sole Carbon Source.

    abstract::Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

    更新日期:2016-01-01 00:00:00

  • Molecular Cloning and Characterization of a Novel Cold-Adapted Alkaline 1,3-α-3,6-Anhydro-L-galactosidase, Ahg558, from Gayadomonas joobiniege G7.

    abstract::Agar, a major polysaccharide of red algal cells, is degraded by β-agarases into neoagarobiose, which is further hydrolyzed into the monomers, D-galactose and 3,6-anhydro-L-galactose, by 1,3-α-3,6-anhydro-L-galactosidases including α-1,3-L-neoagarooligasaccharide hydrolase (α-NAOSH). A novel cold-adapted alkaline α-NAO...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Asghar S,Lee CR,Chi WJ,Kang DK,Hong SK

    更新日期:2019-08-01 00:00:00

  • Adaptive evolution of Escherichia coli inactivated in the phosphotransferase system operon improves co-utilization of xylose and glucose under anaerobic conditions.

    abstract::Modification of the phosphoenolpyruvate/sugar phosphotransferase system (PTS) has shown improvement in sugar coassimilation in Escherichia coli production strains. However, in preliminary experiments under anaerobic conditions, E. coli strains with an inactive PTS and carrying pLOI1594, which encodes pyruvate decarbox...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Balderas-Hernández VE,Hernández-Montalvo V,Bolívar F,Gosset G,Martínez A

    更新日期:2011-02-01 00:00:00

  • Ultrafiltration of thin stillage from conventional and e-mill dry grind processes.

    abstract::We used ultrafiltration (UF) to evaluate membrane filtration characteristics of thin stillage and determine solids and nutrient compositions of filtered streams. To obtain thin stillage, corn was fermented using laboratory methods. UF experiments were conducted in batch mode under constant temperature and flow rate co...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Arora A,Dien BS,Belyea RL,Wang P,Singh V,Tumbleson ME,Rausch KD

    更新日期:2011-05-01 00:00:00

  • Statistical analysis and modeling of pelletized cultivation of Mucor circinelloides for microbial lipid accumulation.

    abstract::Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Xia C,Wei W,Hu B

    更新日期:2014-04-01 00:00:00

  • Elucidation of fluoranthene degradative characteristics in a newly isolated Achromobacter xylosoxidans DN002.

    abstract::Strain DN002 isolated from petroleum-contaminated soil was identified as Achromobacter xylosoxidans based on morphological and biochemical properties and 16S rRNA phylogeny, and investigated for its potential to utilize numerous polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene and pyrene as sole carbon and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ma YL,Lu W,Wan LL,Luo N

    更新日期:2015-02-01 00:00:00

  • In vitro refolding of triosephosphate isomerase from L. donovani.

    abstract::The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate w...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kumar K,Bhargava P,Roy U

    更新日期:2011-08-01 00:00:00

  • Production of fumaric acid from L-malic acid by solvent engineering using a recombinant thermostable fumarase from Thermus thermophilus HB8.

    abstract::Currently, fumaric acid is produced by catalytic isomerization of maleic acid in aqueous solutions at low pH. Being petroleum based, requiring catalyst, and producing vast amounts of by-products and wastewater, the production of fumaric acid from renewable resources by a "green" process is increasingly attractive. In ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu Y,Song J,Tan T,Liu L

    更新日期:2015-03-01 00:00:00

  • Modeling a protein foam fractionation process.

    abstract::A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liq...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Du L,Loha V,Tanner RD

    更新日期:2000-04-01 00:00:00

  • Potentiometric response of lipid modified ISFET.

    abstract::The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Osa T

    更新日期:1993-04-01 00:00:00