New method showing the influence of matrix components in Leuconostoc mesenteroides biofilm formation.

Abstract:

:Studying biofilm formation and influence of the matrix composition was heavy because only old and long methods were employed up to now: confocal microscopy, fluorescent chemical markers, and/or dying techniques. In this context, an innovative tool, the BioFilm Ring Test, was here employed to explore the role of exopolysaccharides, proteins, and nucleic acids in the formation of biofilm by Leuconostoc mesenteroides. The principle is to add magnetic particles in the culture medium. When a biofilm is formed, particles are unable to migrate in the media to form a ring when a magnet is brought nearer to the well. Therefore, culture media supplemented with proteases, glycanases, and/or nucleases allowed us to identify the involvement of these substances in L. mesenteroides biofilm formation. The results permitted to demonstrate that dextran, proteins, and nucleic acids are implied in biofilm formation.

authors

Badel S,Laroche C,Gardarin C,Bernardi T,Michaud P

doi

10.1007/s12010-008-8199-y

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

364-70

issue

2-3

eissn

0273-2289

issn

1559-0291

journal_volume

151

pub_type

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