Site-directed mutagenesis improves the thermostability and catalytic efficiency of Aspergillus niger N25 phytase mutated by I44E and T252R.

abstract：:An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination ...

journal_title：Applied biochemistry and biotechnology

authors： Fatima N,Ahmad N,Ahmad I,Anis M

更新日期：2015-09-01 00:00:00

abstract：:A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...

journal_title：Applied biochemistry and biotechnology

authors： Caudan C,Filali A,Lefebvre D,Spérandio M,Girbal-Neuhauser E

更新日期：2012-04-01 00:00:00

abstract：:The ability of non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment to adsorb As (III) from aqueous solution in batch experiments was investigated as a function of contact time, initial As (III) concentration, pH, temperature and biomass dosage. Langmuir mod...

journal_title：Applied biochemistry and biotechnology

authors： Mohd Bahari Z,Ali Hamood Altowayti W,Ibrahim Z,Jaafar J,Shahir S

更新日期：2013-12-01 00:00:00

abstract：:This study presents the production of biodiesel from algae oil by transesterification using thermophilic microorganism. The microorganism used in this study was isolated from the soil sample obtained near the furnace. The organism was identified as Bacillus sp., and the lipase obtained was purified by ammonium sulfate...

journal_title：Applied biochemistry and biotechnology

authors： Sivaramakrishnan R,Muthukumar K

更新日期：2012-02-01 00:00:00

abstract：:Urease has been immobilized and layered onto the walls of manufactured silicon microchannels. Enzyme immobilization was performed using layer-by-layer nano self-assembly. Alternating layers of oppositely charged polyelectrolytes, with enzyme layers "encased" between them, were deposited onto the walls of the silicon m...

journal_title：Applied biochemistry and biotechnology

authors： Forrest SR,Elmore BB,Palmer JD

更新日期：2005-04-01 00:00:00

abstract：:Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPE...

journal_title：Applied biochemistry and biotechnology

authors： Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen W

更新日期：2014-05-01 00:00:00

abstract：:During the aging of yeast culture, Kluyveromyces marxianus undergoes a number of changes in physiology and these changes play a significant role during fermentation. Aged stationary phase cells were found to contain more reactive oxygen species. Additionally, the level of oxidant is counteracted by the antioxidant def...

journal_title：Applied biochemistry and biotechnology

authors： Saini P,Beniwal A,Vij S

更新日期：2017-09-01 00:00:00

abstract：:The short supply of edible vegetable oils is the limiting factor in the progression of biodiesel technology; thus, in this study, we applied response surface methodology in order to optimize the reaction factors for biodiesel synthesis from inedible castor oil. Specifically, we evaluated the effects of multiple parame...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH

更新日期：2009-05-01 00:00:00

abstract：:As a part of a natural biological N-cycle, nitrification is one of the steps included in the conception of artificial ecosystems designed for extraterrestrial life support systems (LSS) such as Micro-Ecological Life Support System Alternative (MELiSSA) project, which is the LSS project of the European Space Agency. Ni...

journal_title：Applied biochemistry and biotechnology

authors： Farges B,Poughon L,Roriz D,Creuly C,Dussap CG,Lasseur C

更新日期：2012-07-01 00:00:00

abstract：:The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

journal_title：Applied biochemistry and biotechnology

authors： Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

更新日期：2013-01-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

journal_title：Applied biochemistry and biotechnology

authors： Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

更新日期：1984-01-01 00:00:00

abstract：:Gold nanomaterials (Au NMs) have attracted increasing attention in biomedicine due to their facile preparation, multifunctional modifications, unique optical and electrical properties, and good biocompatibility. The physicochemical properties of Au NMs at nanoscale, like size, shape, surface chemistry, and near field ...

journal_title：Applied biochemistry and biotechnology

authors： Jiang XM,Wang LM,Wang J,Chen CY

更新日期：2012-03-01 00:00:00

abstract：:Pseudomonas are ubiquitous bacteria that can live under a wide range of environmental conditions; some species are successfully used in the fermentation industry. Pseudomonas plecoglossicida JUIM01 strain is an important industrial 2-ketogluconate (2KGA) producer. In Pseudomonas, kguT gene encodes a putative 2KGA tran...

journal_title：Applied biochemistry and biotechnology

authors： Sun W,Wang Q,Luan F,Man Z,Cui F,Qi X

更新日期：2019-03-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the mo...

journal_title：Applied biochemistry and biotechnology

authors： Jana A,Maity C,Halder SK,Mondal KC,Pati BR,Mohapatra PK

更新日期：2012-07-01 00:00:00

abstract：:The changes in total phenolics, flavonoids, tannins, valerenic acid, and antioxidant activity were assessed in 25 populations of Valeriana jatamansi sampled from 1200 to 2775 m asl and four habitat types of Uttarakhand, West Himalaya. Significant (p < 0.05) variations in total phenolics, flavonoids, valerenic acid, an...

journal_title：Applied biochemistry and biotechnology

authors： Jugran AK,Bahukhandi A,Dhyani P,Bhatt ID,Rawal RS,Nandi SK

更新日期：2016-07-01 00:00:00

abstract：:The ethanologenic bacteria Escherichia coli strains KO11 and LYO1, and Klebsiella oxytoca strain P2, were investigated for their ability to metabolize furfural. Using high performance liquid chromatography and 13C-nuclear magnetic resonance spectroscopy, furfural was found to be completely biotransformed into furfuryl...

journal_title：Applied biochemistry and biotechnology

authors： Gutiérrez T,Buszko ML,Ingram LO,Preston JF

更新日期：2002-04-01 00:00:00

abstract：:Atomic force microscopy was used to study the geometric structure of collagen fibrils and molecules of rat calcanean tendon tissues. The authors found that the diameter of the fibrils ranged from 124 to 170 nm, and their geometric form suggested a helical winding with spectral period from 59.4 to 61.7 nm, close to the...

journal_title：Applied biochemistry and biotechnology

authors： Baranauskas V,Vidal BC,Parizotto NA

更新日期：1998-02-01 00:00:00

abstract：:6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of D-glucose. They serve as precursors of the antimicrobial α-methylene-γ-butyrolactones tulipalins A (PaA) and B (PaB). The conversions of 6-PosA/6-PosB to PaA/PaB are catalyz...

journal_title：Applied biochemistry and biotechnology

authors： Nomura T,Ogita S,Kato Y

更新日期：2019-05-01 00:00:00

abstract：:The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...

journal_title：Applied biochemistry and biotechnology

authors： Yi X,Luo K,Yang Q,Li XM,Deng WG,Cheng HB,Wang ZL,Zeng GM

更新日期：2013-11-01 00:00:00

abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

更新日期：2016-07-01 00:00:00

abstract：:A unicellular marine green alga, Chlamydomonas perigranulata, was demonstrated to synthesize starch through photosynthesis, store it in a cell, and ferment it under anaerobic conditions in the dark to produce ethanol, 2,3-butanediol (butanediol), acetic acid, and carbon dioxide (CO2). Previous fermentation data of an ...

journal_title：Applied biochemistry and biotechnology

authors： Hon-Nami K

更新日期：2006-04-01 00:00:00

abstract：:An economic evaluation model was developed in the Laboratory of Agricultural Structures (LAS) of the Agricultural University of Athens-the Modified Basic Economic Evaluation Model (MBEEM). This model is an improved version of the original Basic Economic Evaluation Model, available in LAS, and it is used to assess the ...

journal_title：Applied biochemistry and biotechnology

authors： Georgakakis D,Christopoulou N,Chatziathanassiou A,Venetis T

更新日期：2003-04-01 00:00:00

abstract：:We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of tyrosinase. TFE increased the degree of secondary structure of tyrosinase, which directly resulted in enzyme inactivation. A reciprocal study showed that TFE inhibited tyrosinase in a slope-parabolic mixed-type inhibition m...

journal_title：Applied biochemistry and biotechnology

authors： Lü ZR,Shi L,Wang J,Park D,Bhak J,Yang JM,Park YD,Zhou HW,Zou F

更新日期：2010-04-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00