Enhanced hydrolysis and acidification of waste activated sludge by biosurfactant rhamnolipid.

abstract：:A newly isolated bacterial strain, Bacillus sp. MX47, was actively producing extracellular xylanase only in xylan-containing medium. The xylanase was purified from the culture broth by two chromatographic steps. The xylanase had an apparent molecular weight of 26.4 kDa with an NH(2)-terminal sequence (Gln-Gly-Gly-Asn-...

journal_title：Applied biochemistry and biotechnology

authors： Chi WJ,Park DY,Chang YK,Hong SK

更新日期：2012-10-01 00:00:00

abstract：:Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented fo...

journal_title：Applied biochemistry and biotechnology

authors： Rudolf A,Galbe M,Lidén G

更新日期：2004-04-01 00:00:00

abstract：:The glpX gene (Rv1099c) of Mycobacterium tuberculosis (Mtb) encodes Fructose 1,6-bisphosphatase II (FBPase II; EC 3.1.3.11); a key gluconeogenic enzyme. Mtb possesses glpX homologue as the major known FBPase. This study explored the expression, purification and enzymatic characterization of functionally active FBPase ...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Rukseree K,Wheeler PR,Franzblau SG,Movahedzadeh F

更新日期：2011-08-01 00:00:00

abstract：:The use of immobilized enzymes has opened the possibility of large scale utilization of NAD(+)-linked dehydrogenases, but the applications of this technique were limited by the necessity of providing the large amounts of NAD(+) required by its stoichiometric consumption in the reaction. After immobilization of alcohol...

journal_title：Applied biochemistry and biotechnology

authors： Burstein C,Ounissi H,Legoy MD,Gellf G,Thomas D

更新日期：1981-12-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT, EC 2.6.1.1) was bound on CNBr-activated Sepharose and the effects of immobilization on the maximum velocity, biologically active pyridoxal-5'-phosphate (PLP), and transaminationable active centers were studied. By comparing these parameters of soluble and immobilized enzyme the facto...

journal_title：Applied biochemistry and biotechnology

authors： Kurkijärvi K,Korpela T

更新日期：1983-04-01 00:00:00

abstract：:Penicillin acylase of E. coli NCIM 2400 has been purified to homogeneity using a combination of hydrophobic interaction chromatography and DEAE-cellulose treatment. A variety of substituted matrices were synthesized using D- or DL-phenylglycine, norleucine, ampicillin, or amoxycillin as ligands, all of which retained ...

journal_title：Applied biochemistry and biotechnology

authors： Mahajan PB,Borkar PS

更新日期：1984-10-01 00:00:00

abstract：:Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

journal_title：Applied biochemistry and biotechnology

authors： Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

更新日期：2014-04-01 00:00:00

abstract：:A hyperthermophilic alpha-amylase encoding gene from Pyrococcus woesei was transferred and expressed in Xanthomonas campestris ATCC 13951. The heterologous alpha-amylase activity was detected in the intracellular fraction of X. campestris and presented similar thermostability and catalytic properties with the native P...

journal_title：Applied biochemistry and biotechnology

authors： Konsoula Z,Liakopoulou-Kyriakides M,Perysinakis A,Chira P,Afendra A,Drainas C,Kyriakidis DA

更新日期：2008-05-01 00:00:00

abstract：:The lead (II) biosorption potential of Aspergillus parasiticus fungal biomass has been investigated in a batch system. The initial pH, biosorbent dosage, contact time, initial metal ion concentrations and temperature were studied to optimize the biosorption conditions. The maximum lead (II) biosorption capacity of the...

journal_title：Applied biochemistry and biotechnology

authors： Akar T,Tunali S,Cabuk A

更新日期：2007-03-01 00:00:00

abstract：:A two-phase partitioning bioreactor to treat gas effluents polluted by volatile organic compound has been developed. In this work, both the mass transfer of isopropylbenzene (IPB) and oxygen have been considered in relation to their influence on the hydrodynamics of the reactor and the type of silicone oils used as a ...

journal_title：Applied biochemistry and biotechnology

authors： Aldric JM,Lecomte JP,Thonart P

更新日期：2009-05-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

journal_title：Applied biochemistry and biotechnology

authors： Kim M,Aita G,Day DF

更新日期：2010-05-01 00:00:00

abstract：:We engineered Rhizopus chinensis lipase to study its critical amino acid role in catalytic properties. Based on the amino acid sequence and three-dimensional model of the lipase, residues located in its lid hinge region (Met93 and Thr96) were replaced with corresponding amino acid residues (Ile93 and Asn96) found in t...

journal_title：Applied biochemistry and biotechnology

authors： Zhu SS,Li M,Yu X,Xu Y

更新日期：2013-05-01 00:00:00

abstract：:Jonesia denitrificans BN-13 produces six xylanases: Xyl1, Xyl2, Xyl3, Xyl4, Xyl5, and Xyl6; the Xyl4 was purified and characterized after two consecutive purification steps using ultrafiltration and anion exchange chromatography. The xylanase-specific activity was found to be 77 unit (U)/mg. The molecular weight of th...

journal_title：Applied biochemistry and biotechnology

authors： Boucherba N,Gagaoua M,Copinet E,Bettache A,Duchiron F,Benallaoua S

更新日期：2014-03-01 00:00:00

abstract：:In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...

journal_title：Applied biochemistry and biotechnology

authors： Hahn-Hägerdal B,Lindén T,Senac T,Skoog K

更新日期：1991-04-01 00:00:00

abstract：:A comparative proteomic analysis was carried out to explore the molecular mechanisms of responses to cold stress in Phalaenopsis after treated by low temperature (13/8 °C day/night) for 15 days. Differentially expressed proteins were examined using two-dimensional electrophoresis (2-DE) and matrix assisted laser desor...

journal_title：Applied biochemistry and biotechnology

authors： Yuan XY,Liang F,Jiang SH,Wan MF,Ma J,Zhang XY,Cui B

更新日期：2015-01-01 00:00:00

abstract：:The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Lee S,Yoon YJ,Lee J

更新日期：2013-01-01 00:00:00

abstract：:Tobacco plants can be used for the production of proteins for pharmaceutical applications. One of the most difficult and expensive tasks associated with this technology is isolating the product of interest from the hundreds of other chemicals found in tobacco. We describe a new recovery strategy in which the protein o...

journal_title：Applied biochemistry and biotechnology

authors： Crofcheck C,Maiti I,Pattanaik S,Jay M

更新日期：2004-10-01 00:00:00

abstract：:Cancer normally tends to result in the decrease of tissue elasticity; i.e., the cancerous region is more rigid than the normal surrounding areas. This would appear as differences in the distribution of internal birefringence that could be used to improve image contrast between the cancerous and normal tissue structure...

journal_title：Applied biochemistry and biotechnology

authors： Mathur N,Chaudhary NK,Bharti P

更新日期：2009-11-01 00:00:00

abstract：:A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...

journal_title：Applied biochemistry and biotechnology

authors： Webb OF,Phelps TJ,Bienkowski PR,DiGrazia PM,Reed GD,Applegate B,White DC,Sayler GS

更新日期：1991-04-01 00:00:00

abstract：:The recovery of alpha-amylase from the crude enzyme preparation by the reversed micellar liquid-liquid extraction was investigated. The reversed micellar solution was formed by dissolving a cationic surfactant Aliquat 336 in six different alkanes (cyclohexane, n-hexane, isooctane, n-octane, n-decane, and n-dodecane) r...

journal_title：Applied biochemistry and biotechnology

authors： Chang QL,Chen JY

更新日期：1997-02-01 00:00:00

abstract：:Artemisinin is an important drug commonly used in the treatment of malaria as a combination therapy. It is primarily produced by a plant Artemisia annua, however, its supply from plant is significantly lower than its huge demand and therefore alternative in vitro production routes are sought. Hairy root cultivation co...

journal_title：Applied biochemistry and biotechnology

authors： Patra N,Srivastava AK

更新日期：2014-11-01 00:00:00

abstract：:Carboxypeptidase N-Sepharose was prepared by covalent immobilization of purified human plasma carboxypeptidase N. More than 98% of the carboxypeptidase N was immobilized; 42% of the applied activity can be detected on the support. The column has excellent capabilities to quantitatively remove carboxy-terminal basic am...

journal_title：Applied biochemistry and biotechnology

authors： Wang W,Hendriks DF,Scharpé SL

更新日期：1994-02-01 00:00:00

abstract：:Proteases constitute one of the most important groups of industrial enzymes, accounting for at least 25% of the total enzyme sales, with two-thirds of the proteases produced commercially being of microbial origin. Immobilized enzymes are currently the subject of considerable interest because of their advantages over s...

journal_title：Applied biochemistry and biotechnology

authors： Kirkkopru I,Alpaslan C,Omay D,Güvenilir Y

更新日期：2006-04-01 00:00:00

abstract：:A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

更新日期：1992-09-01 00:00:00

abstract：:A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) follow...

journal_title：Applied biochemistry and biotechnology

authors： Santi-Gadelha T,Rocha BA,Oliveira CC,Aragão KS,Marinho ES,Gadelha CA,Toyama MH,Pinto VP,Nagano CS,Delatorre P,Martins JL,Galvani FR,Sampaio AH,Debray H,Cavada BS

更新日期：2008-07-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:Oil palm empty fruit bunch (EFB) is abundantly available in Malaysia and it is a potential source of xylose for the production of high-value added products. This study aimed to optimize the hydrolysis of EFB using dilute sulfuric acid (H2SO4) and phosphoric acid (H3PO4) via response surface methodology for maximum xyl...

journal_title：Applied biochemistry and biotechnology

authors： Tan HT,Dykes GA,Wu TY,Siow LF

更新日期：2013-08-01 00:00:00

abstract：:Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectrosc...

journal_title：Applied biochemistry and biotechnology

authors： Türkhan A,Kaya ED,Koçyiğit A

更新日期：2020-10-01 00:00:00