Novel approaches to the purification of penicillin acylase.

abstract：:Xanthomonas campestris w.t. was used for production of xanthan gum in fermentations with chestnut flour for the first time. Fermentations were carried out with either chestnut flour or its soluble sugars (33.5%) and starch (53.6%), respectively, at 28 degrees C and 200 rpm at initial pH 7.0 in flasks. The effect of ag...

journal_title：Applied biochemistry and biotechnology

authors： Liakopoulou-Kyriakides M,Psomas SK,Kyriakidis DA

更新日期：1999-12-01 00:00:00

abstract：:Streptomyces griseorubens JSD-1 is a novel actinomycete that could grow efficiently upon lignin, and the ligninolytic genes active in this biotransformation were expected to be crucial. To investigate the molecular mechanism of utilizing lignin, genome sequencing was carried out to obtain its draft genome, which was d...

journal_title：Applied biochemistry and biotechnology

authors： Feng H,Zhang D,Sun Y,Zhi Y,Mao L,Luo Y,Xu L,Wang L,Zhou P

更新日期：2015-05-01 00:00:00

abstract：:Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

journal_title：Applied biochemistry and biotechnology

authors： Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

更新日期：2012-07-01 00:00:00

abstract：:For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...

journal_title：Applied biochemistry and biotechnology

authors： Zhao L,Jiang Y,Fang H,Zhang H,Cheng S,Rajoka MSR,Wu Y

更新日期：2019-10-01 00:00:00

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang JF,Yang B,Chen W,Chen J

更新日期：2014-04-01 00:00:00

abstract：:Chicken feathers are available in large quantities around the world causing environmental challenges. The feathers are composed of keratin that is a recalcitrant protein and is hard to degrade. In this work, chicken feathers were aerobically pretreated for 2-8 days at total solid concentrations of 5, 10, and 20 % by B...

journal_title：Applied biochemistry and biotechnology

authors： Patinvoh RJ,Feuk-Lagerstedt E,Lundin M,Sárvári Horváth I,Taherzadeh MJ

更新日期：2016-12-01 00:00:00

abstract：:Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

journal_title：Applied biochemistry and biotechnology

authors： Nghiem NP,Cofer TM

更新日期：2007-05-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...

journal_title：Applied biochemistry and biotechnology

authors： Zanin GM,De Moraes FF

更新日期：1998-04-01 00:00:00

abstract：:In this study, benzene, toluene, and xylenes (BTX) removal from contaminated water by physical, chemical, and biological processes was studied. Results showed that air sparging in polluted water can reduce monoaromatic compounds from 140,000 to about 5 microg/l in only 1 h process with a gassing rate of 0.33 VVM. This...

journal_title：Applied biochemistry and biotechnology

authors： Farhadian M,Duchez D,Vachelard C,Larroche C

更新日期：2008-12-01 00:00:00

abstract：:The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Jones F,Bailey R,Wilson S,Hiestand J

更新日期：2007-04-01 00:00:00

abstract：:Hexavalent chromium, Cr(6+), is a common and toxic pollutant in soils and waters. Reduction of the mobile Cr(6+) to the less mobile and less toxic trivalent chromium, Cr(3+), can be achieved with conventional chemical reduction technologies. Alternatively, Cr(6+) can be biochemically reduced to Cr(3+) by anaerobic mic...

journal_title：Applied biochemistry and biotechnology

authors： Turick CE,Camp CE,Apel WA

更新日期：1997-04-01 00:00:00

abstract：:Label-free detection technique based on impedance was investigated for aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2) analysis in milk products. The impedance change resulting from antigen-antibody interaction was studied using a two-electrode setup made up of silver (Ag) wire. Processed milk such as drinking yogurt and ...

journal_title：Applied biochemistry and biotechnology

authors： Kanungo L,Bacher G,Bhand S

更新日期：2014-10-01 00:00:00

abstract：:The aim of the present study was bioremediation of distillery sludge into a soil-enriching material. It was mixed with a complementary waste, cattle dung, and subjected to vermicomposting with (V) and without (T, control) Eisenia fetida in the ratio of 0:100 % (V1, T1), 10:90 (V2, T2), 25:75 (V3, T3), 50:50 (V4, T4), ...

journal_title：Applied biochemistry and biotechnology

authors： Singh J,Kaur A,Vig AP

更新日期：2014-10-01 00:00:00

abstract：:Response surface methodology was employed to optimize culture medium for production of lipase with Rhodotorula sp. MTCC 8737. In the first step, a Plackett-Burman design was used to evaluate the effects of different inducers qualitatively. Of all the seven inducers tested, soybean oil showed significant influence on t...

journal_title：Applied biochemistry and biotechnology

authors： Chennupati S,Potumarthi R,Gopal Rao M,Manga PL,Sridevi M,Jetty A

更新日期：2009-11-01 00:00:00

abstract：:Sequencing batch reactors (SBRs) including aerobic SBRs and anaerobic SBRs (ASBRs) are partially emptied batch reactors that are widely used as bioprocesses in pollution control. We present dilution and biochemical materials balance modeling equations and simulation results for the partially emptied batch reactors, es...

journal_title：Applied biochemistry and biotechnology

authors： Ndon UJ,Zhao JC,Siguan ML

更新日期：2007-01-01 00:00:00

abstract：:Polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. In this study, specific bacterial strains produced specific copolymers from food waste. Copolymers of HB and HV (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]...

journal_title：Applied biochemistry and biotechnology

authors： Wong PA,Chua H,Lo W,Lawford HG,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Based on the principle of self-coagulation of microorganisms, the flocculant-producing denitrifying bacterial TN-14 sludge was added to the continuous-flow reactor for treating domestic sewage. The bacterial TN-14 sludge acted as the main seed sludge to promote the rapid formation of aerobic granular sludge. The sludg...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Lu H,Yao L,Leng L,Guan L

更新日期：2017-01-01 00:00:00

abstract：:A new label--laccase from the fungus Coriolus hirsutus--was applied for solid-phase enzyme-linked immunosorbent assays of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D). Two proposed assays are based on (1) competitive binding of antibody-laccase conjugate with immobilized 2,4-D-protein conjugate and 2,4-D in te...

journal_title：Applied biochemistry and biotechnology

authors： Zherdev AV,Bizova NA,Yaropolov AI,Lyubimova NV,Morozova OV,Dzantiev BB

更新日期：1999-03-01 00:00:00

abstract：:The notch signaling pathway plays an important role in inhibiting cell differentiation and enhancing the repopulation capability of hematopoietic stem/progenitor cells. In this study, we developed rhDSL, a novel soluble form of Notch ligand Delta-like-1, which contains the DSL domain and the N-terminal sequence of the...

journal_title：Applied biochemistry and biotechnology

authors： Zhao M,Wu M,Guo L,Jiang J,Huang W,Lin X,Zhang Z,Xiang D,Lu H,Zhu S,Yu Y,Moldenhauer A,Han W

更新日期：2010-03-01 00:00:00

abstract：:This study was carried out to evaluate the capacity of anaerobic granular sludge for oxidizing ammonium and p-cresol with nitrate as terminal electron acceptor. Kinetics for the anaerobic oxidation of ammonium and p-cresol is described in this paper. The phenolic compound was very efficiently consumed, achieving 65 % ...

journal_title：Applied biochemistry and biotechnology

authors： González-Blanco G,Cervantes FJ,Beristain-Cardoso R,Gómez J

更新日期：2015-08-01 00:00:00

abstract：:As technologies for utilizing biomass for fuel and chemical production continue to improve, enzymatic hydrolysis can be run at still higher solids concentrations. For hydrolyses that initially contain little or no free water (10-40% total solids, w/w), the saccharification of insoluble polymers into soluble sugars inv...

journal_title：Applied biochemistry and biotechnology

authors： Kristensen JB,Felby C,Jørgensen H

更新日期：2009-05-01 00:00:00

abstract：:Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation. Presence of silic...

journal_title：Applied biochemistry and biotechnology

authors： Krishnamoorthy S,Iyer NR,Murthy AR

更新日期：2015-02-01 00:00:00

abstract：:The factors affecting decolorization of anthraquinone dye represented by Reactive Blue 4 (RB4) and azo dye represented by Methyl Orange (MO) were studied in batch experiments under mesophilic (35 degrees C) and thermophilic (55 degrees C) anaerobic conditions. The results indicated differences in decolorization proper...

journal_title：Applied biochemistry and biotechnology

authors： Boonyakamol A,Imai T,Chairattanamanokorn P,Higuchi T,Sekine M

更新日期：2009-07-01 00:00:00

abstract：:Hydrogen (H2) production from the organic fraction of solid waste such as fruit and vegetable waste (FVW) is a novel and feasible energy technology. Continuous application of this process would allow for the simultaneous treatment of organic residues and energy production. In this study, batch experiments were conduct...

journal_title：Applied biochemistry and biotechnology

authors： Garcia-Peña EI,Canul-Chan M,Chairez I,Salgado-Manjarez E,Aranda-Barradas J

更新日期：2013-09-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:Pectinases catalyze the degradation of pectic substances and are used in several processes, mainly in food and textile industries. In this study, a biomimetic matrix of alginate/gelatin/calcium oxalate (AGOCa) was synthesized for the in situ immobilization via encapsulation of crude pectinase from Aspergillus niger AT...

journal_title：Applied biochemistry and biotechnology

authors： Bustamante-Vargas CE,de Oliveira D,Valduga E,Venquiaruto LD,Paroul N,Backes GT,Dallago RM

更新日期：2016-07-01 00:00:00

abstract：:EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully iso...

journal_title：Applied biochemistry and biotechnology

authors： Fang HY,Chen SC,Chen SL

更新日期：2003-11-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in...

journal_title：Applied biochemistry and biotechnology

authors： Linhardt RJ,Cooney CL,Tapper D,Zannetos CA,Larsen AK,Langer R

更新日期：1984-02-01 00:00:00