Molecular modification of Protein A to improve the elution pH and alkali resistance in affinity chromatography.

abstract：:This study deals with the utilization of chicken feather waste as a substrate for anaerobic digestion and improving biogas production by degradation of the compact structure of the feather keratin. In order to increase the digestibility of the feather, different pretreatments were investigated, including thermal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Forgács G,Lundin M,Taherzadeh MJ,Sárvári Horváth I

更新日期：2013-04-01 00:00:00

abstract：:For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...

journal_title：Applied biochemistry and biotechnology

authors： Zhao L,Jiang Y,Fang H,Zhang H,Cheng S,Rajoka MSR,Wu Y

更新日期：2019-10-01 00:00:00

abstract：:Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

journal_title：Applied biochemistry and biotechnology

authors： Rondags E,Halliday E,Marc I

更新日期：1998-02-01 00:00:00

abstract：:The objective of the present work was to evaluate the metabolic effects induced by the initial glucose concentration (IGC) on the cultivation of Streptococcus zooepidemicus for the production of hyaluronic acid (HA). These effects were monitored along non-controlled pH cultivations, carried out in 250-mL Erlenmeyer fl...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Santana MH

更新日期：2010-11-01 00:00:00

abstract：:The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: 1.1869) was isolated from the aeration tank water of a sewage treatment plant. SSE1 showed morphological fe...

journal_title：Applied biochemistry and biotechnology

authors： Lu H,Liu H,Lu M,Wang J,Liu X,Liu R

更新日期：2020-09-01 00:00:00

abstract：:Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structu...

journal_title：Applied biochemistry and biotechnology

authors： Xu P,Yuan R,Hou G,Li J,Ye M

更新日期：2018-06-01 00:00:00

abstract：:Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...

journal_title：Applied biochemistry and biotechnology

authors： Shah A,Priyadarshini M,Khan MS,Aatif M,Amin F,Bano B

更新日期：2013-10-01 00:00:00

abstract：:A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

更新日期：1992-09-01 00:00:00

abstract：:Human acidic fibroblast growth factor (haFGF) is a multifunctional protein involved in regulating a wide range of cellular processes. As a potent therapeutic agent, it is highly desirable to produce recombinant haFGF (r-haFGF) at low cost. However, the complex structure and formation of aggregation confines its high-l...

journal_title：Applied biochemistry and biotechnology

authors： Rahman IU,Liu W,Wei Z,Fang L,Zheng X,Huang L,Xu Z

更新日期：2020-08-01 00:00:00

abstract：:Andrographis paniculata belonging to the family Acanthaceae produces a group of diterpene lactones, one of which is the pharmaceutically important-andrographolide. It is known to possess various important biological properties like anticancer, anti-HIV, anti-inflammatory, etc. This is the first report on the productio...

journal_title：Applied biochemistry and biotechnology

authors： Gandi S,Rao K,Chodisetti B,Giri A

更新日期：2012-12-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:Since the two reports published in 1986 by the laboratories of R. Lerner and P. G. Schultz, it has been clearly established that antibodies may be induced to act as catalysts in numerous chemical reactions. In all cases, catalytic antibodies were elicited using a substrate-based approach. In the present article, we pr...

journal_title：Applied biochemistry and biotechnology

authors： Friboulet A,Izadyar L,Avalle B,Roseto A,Thomas D

更新日期：1994-05-01 00:00:00

abstract：:In plants, the final step of cysteine formation is catalyzed by O-acetylserine (thiol) lyase (OAS-TL). The purpose of this study was to isolate and characterize an OAS-TL from the tree legume Leucaena leucocephala (leucaena). Leucaena contains a toxic, nonprotein amino acid, mimosine, which is also formed by an OAS-TL...

journal_title：Applied biochemistry and biotechnology

authors： Yafuso JT,Negi VS,Bingham JP,Borthakur D

更新日期：2014-07-01 00:00:00

abstract：:Oxidation of lignin obtained from acetosolv and ethanol/water pulping of sugarcane bagasse was performed by phenol oxidases: tyrosinase (TYR) and laccase (LAC), to increase the number of carbonyl and hydroxyl groups in lignin, and to improve its chelating capacity. The chelating properties of the original and oxidized...

journal_title：Applied biochemistry and biotechnology

authors： Calabria GM,Gonçalves AR

更新日期：2006-04-01 00:00:00

abstract：:Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...

journal_title：Applied biochemistry and biotechnology

authors： Li SW,Li M,Song HP,Feng JL,Tai XS

更新日期：2011-10-01 00:00:00

abstract：:Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

journal_title：Applied biochemistry and biotechnology

authors： Bresolin ITL,Bresolin IRAP,Bueno SMA

更新日期：2020-06-01 00:00:00

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:Rice straw hemicellulosic hydrolysate was used as fermentation medium for ethanol production by Pichia stipitis NRRL Y-7124. Shaking bath experiments were initially performed aiming to establish the best initial xylose concentration to be used in this bioconversion process. In the sequence, assays were carried out und...

journal_title：Applied biochemistry and biotechnology

authors： Silva JP,Mussatto SI,Roberto IC

更新日期：2010-11-01 00:00:00

abstract：:A novel immobilized-enzyme system that has glucoamylase on the surface of, and glucose oxidase within, polyurea microcapsules was developed. This system was found to carry out its sequential enzymatic reaction effectively. It was also demonstrated that the use of microcapsules was useful for this immobilized-enzyme sy...

journal_title：Applied biochemistry and biotechnology

authors： Komori T,Muramatsu N,Kondo T

更新日期：1987-02-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:Potassium acetate is currently made by reacting petroleum-based acetic acid with potassium hydroxide. An alternate process, anaerobic fermentation of dextrose with Clostridium thermoaceticum, could be used and could possibly be cheaper. Growth characteristics and productivity of the fermentation were optimized to maxi...

journal_title：Applied biochemistry and biotechnology

authors： Shah MM,Akanbi F,Cheryan M

更新日期：1997-04-01 00:00:00

abstract：:Ovarian cancer lacks clear syndromes at an early stage and could result in serious problem in woman's health status. The current diagnostic approach relies on physical examination, ultrasound examination, and blood test for CA125. These approaches could not diagnose early stage ovarian cancer with high sensitivity and...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Y,Guo B,Bi R

更新日期：2012-10-01 00:00:00

abstract：:The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...

journal_title：Applied biochemistry and biotechnology

authors： Selma VC,Cotrim LH,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-12-01 00:00:00

abstract：:Carboxypeptidase N-Sepharose was prepared by covalent immobilization of purified human plasma carboxypeptidase N. More than 98% of the carboxypeptidase N was immobilized; 42% of the applied activity can be detected on the support. The column has excellent capabilities to quantitatively remove carboxy-terminal basic am...

journal_title：Applied biochemistry and biotechnology

authors： Wang W,Hendriks DF,Scharpé SL

更新日期：1994-02-01 00:00:00

abstract：:This study proposes an innovative strategy of lignocellulose biodegradation by Inonotus obliquus under solid-state fermentation in extracting Eucommia ulmoides trans-1,4-polyisoprene (EUG) and producing reducing sugars efficiently. EUG and sugars were obtained through the white rot fungal pretreatment of E. ulmoides l...

journal_title：Applied biochemistry and biotechnology

authors： Qian S,Zhang C,Zhu Z,Huang P,Xu X

更新日期：2020-11-01 00:00:00

abstract：:A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in...

journal_title：Applied biochemistry and biotechnology

authors： Linhardt RJ,Cooney CL,Tapper D,Zannetos CA,Larsen AK,Langer R

更新日期：1984-02-01 00:00:00

abstract：:The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed o...

journal_title：Applied biochemistry and biotechnology

authors： Murhammer DW

更新日期：1991-12-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:A high cellobiohydrolase (CBH)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 according to the morphology and comparison of internal transcribed spacer rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, a maximum CBH activi...

journal_title：Applied biochemistry and biotechnology

authors： Lee KM,Joo AR,Jeya M,Lee KM,Moon HJ,Lee JK

更新日期：2011-01-01 00:00:00

abstract：:Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomateri...

journal_title：Applied biochemistry and biotechnology

authors： Khamhaengpol A,Siri S

更新日期：2017-09-01 00:00:00