A novel immobilized-enzyme system utilizing microcapsules.

abstract：:Anaerobic sequencing batch reactors containing granular or flocculent biomass have been employed successfully in the treatment of piggery wastewater. However, the studies in which these reactors were employed did not focus specifically on accelerating the hydrolysis step, even though the degradation of this chemical o...

journal_title：Applied biochemistry and biotechnology

authors： de Pinho SC,Fernandes BS,Rodrigues JA,Ratusznei SM,Foresti E,Zaiat M

更新日期：2005-02-01 00:00:00

abstract：:The unique carbohydrate-binding property of lectins makes them invaluable tools in biomedical research. Here, we report the purification, partial primary structure, carbohydrate affinity characterization, crystallization, and preliminary X-ray diffraction analysis of a lactose-specific lectin from Cymbosema roseum see...

journal_title：Applied biochemistry and biotechnology

authors： Rocha BA,Moreno FB,Delatorre P,Souza EP,Marinho ES,Benevides RG,Rustiguel JK,Souza LA,Nagano CS,Debray H,Sampaio AH,de Azevedo WF Jr,Cavada BS

更新日期：2009-03-01 00:00:00

abstract：:A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

journal_title：Applied biochemistry and biotechnology

authors： Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

更新日期：2008-04-01 00:00:00

abstract：:In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus repo...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Yang SX,Cheng Y,Liu DQ,Zhang Y,Deng KJ,Zheng XL

更新日期：2015-12-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00

abstract：:Urease has been immobilized and layered onto the walls of manufactured silicon microchannels. Enzyme immobilization was performed using layer-by-layer nano self-assembly. Alternating layers of oppositely charged polyelectrolytes, with enzyme layers "encased" between them, were deposited onto the walls of the silicon m...

journal_title：Applied biochemistry and biotechnology

authors： Forrest SR,Elmore BB,Palmer JD

更新日期：2005-04-01 00:00:00

abstract：:A putative α-amylase gene (accession number, CP000284) of Methylobacillus flagellatus KT ATCC51484 was cloned in Escherichia coli, and its gene product was expressed and characterized. The purified recombinant enzyme (MFAS) displayed a typical amylosucrase (ASase) activity by the demonstration of multiple activities o...

journal_title：Applied biochemistry and biotechnology

authors： Jeong JW,Seo DH,Jung JH,Park JH,Baek NI,Kim MJ,Park CS

更新日期：2014-06-01 00:00:00

abstract：:Serratia marcescens SP511 was previously reported to be an L-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L of L-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions...

journal_title：Applied biochemistry and biotechnology

authors： Masuda M,Takamatu S,Nishimura N,Komatsubara S,Tosa T

更新日期：1993-12-01 00:00:00

abstract：:The presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. In the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-p...

journal_title：Applied biochemistry and biotechnology

authors： Daverey A,Pakshirajan K

更新日期：2011-03-01 00:00:00

abstract：:Marinobacter sp. (MSI032) isolated from the marine sponge Dendrilla nigra was optimized for the production of extracellular cellulolytic enzyme (CMCase) by submerged fermentation. Initial experiments showed that the culture medium containing 1% maltose as carbon source and 1% peptone and casein as nitrogen source supp...

journal_title：Applied biochemistry and biotechnology

authors： Shanmughapriya S,Kiran GS,Selvin J,Thomas TA,Rani C

更新日期：2010-10-01 00:00:00

abstract：:In this paper we concentrate our attention on the stability and transient behavior of the isothermal system (CSTR) with a substrate-inhibited enzyme reaction producing hydrogen ions. Our investigation covers the region of multiple steady states uncovered previously (1) (ordinary hysteresis and isola). We investigate t...

journal_title：Applied biochemistry and biotechnology

authors： Elnashaie SS,Elrifaie MA,Ibrahim G,Badra G

更新日期：1983-12-01 00:00:00

abstract：:Polymerization and self-assembly of proteins into nanoaggregates of different sizes and morphologies (nanoensembles or nanofilaments) is a phenomenon that involved problems in various neurodegenerative diseases (medicine) and enzyme instability/inactivity (biotechnology). Thermal polymerization of horse liver alcohol ...

journal_title：Applied biochemistry and biotechnology

authors： Barzegar A,Moosavi-Movahedi AA,Kyani A,Goliaei B,Ahmadian S,Sheibani N

更新日期：2010-02-01 00:00:00

abstract：:In this article, two theories are unified to investigate the effect of hydrodynamics on a specific bioprocess: the network-of-zones (NOZ) hydrodynamic structured modeling approach (developed by several researchers but applied to only a few bioprocesses) and the effectiveness factor eta approach. Two process scales wer...

journal_title：Applied biochemistry and biotechnology

authors： Delvigne F,El Mejdoub T,Destain J,Delroisse JM,Vandenbol M,Haubruge E,Thonart P

更新日期：2005-04-01 00:00:00

abstract：:The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

journal_title：Applied biochemistry and biotechnology

authors： Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

更新日期：1999-04-01 00:00:00

abstract：:The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

journal_title：Applied biochemistry and biotechnology

authors： Osa T

更新日期：1993-04-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00

abstract：:Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N(1) domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical syn...

journal_title：Applied biochemistry and biotechnology

authors： Tang XS,Tang ZR,Wang SP,Feng ZM,Zhou D,Li TJ,Yin YL

更新日期：2012-02-01 00:00:00

abstract：:This work reports the isolation of a serum lectin from cobia fish (Rachycentron canadum) named RcaL. Immunomodulatory activity on mice splenocyte experimental cultures through cytotoxic assays and cytokine production were also performed. RcaL was obtained through precipitation with ammonium sulphate and affinity chrom...

journal_title：Applied biochemistry and biotechnology

authors： Coriolano MC,Silva CD,Melo CM,Bezerra Rde S,Santos AJ,Pereira VR,Coelho LC

更新日期：2012-11-01 00:00:00

abstract：:During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...

journal_title：Applied biochemistry and biotechnology

authors： Xie D,Liu D,Zhu H,Liu T

更新日期：2001-08-01 00:00:00

abstract：:A proteinaceous glycopeptide was isolated from the posterior salivary gland (PSG) of Sepia pharaonis by gel (Sephadex G-100) filtration chromatography and purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Among the collected fractions, fraction 12 showed a retention time (RT) of 31 min. The ...

journal_title：Applied biochemistry and biotechnology

authors： Karthik R,Saravanan R,Ebenezar KK,Sivamalai T

更新日期：2015-02-01 00:00:00

abstract：:In this study, seedling, callus, cell, and adventitious root of Glycyrrhiza uralensis Fisch. have been established. In order to find the best one for producing G. uralensis active constituents, triterpenoid saponins and flavonoids in native root and tissue cultures were determined, and the contents in different G. ura...

journal_title：Applied biochemistry and biotechnology

authors： Wang J,Gao W,Zhang L,Huang L

更新日期：2013-01-01 00:00:00

abstract：:The importance of using tissue-specific promoters in the genetic transformation of plants has been emphasized increasingly. Here, we report the isolation of a novel seed-specific promoter region from peanut and its validation in Arabidopsis and tobacco seeds. The reported promoter region referred to as groundnut seed ...

journal_title：Applied biochemistry and biotechnology

authors： Sunkara S,Bhatnagar-Mathur P,Sharma KK

更新日期：2014-01-01 00:00:00

abstract：:We engineered Rhizopus chinensis lipase to study its critical amino acid role in catalytic properties. Based on the amino acid sequence and three-dimensional model of the lipase, residues located in its lid hinge region (Met93 and Thr96) were replaced with corresponding amino acid residues (Ile93 and Asn96) found in t...

journal_title：Applied biochemistry and biotechnology

authors： Zhu SS,Li M,Yu X,Xu Y

更新日期：2013-05-01 00:00:00

abstract：:Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...

journal_title：Applied biochemistry and biotechnology

authors： Zhang B,Dong C,Shang Q,Cong Y,Kong W,Li P

更新日期：2013-12-01 00:00:00

abstract：:This study evaluates the hydrolysis specificity of newly developed protease from Exiguobacterium sp. SWJS2 (EP) based on the released free amino acid (FAA) during enzymolysis and through comparing with commercially available papain and Alcalase 2.4L. Results showed that EP had great potential in producing hydrolysates...

journal_title：Applied biochemistry and biotechnology

authors： Lei F,Cui C,Zhao Q,Sun-Waterhouse D,Zhao M

更新日期：2014-10-01 00:00:00

abstract：:Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...

journal_title：Applied biochemistry and biotechnology

authors： Brumm P,Mead D,Boyum J,Drinkwater C,Deneke J,Gowda K,Stevenson D,Weimer P

更新日期：2011-03-01 00:00:00

abstract：:The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...

journal_title：Applied biochemistry and biotechnology

authors： Pradhan SP,Paterek JR,Liu BY,Conrad JR,Srivastava VJ

更新日期：1997-04-01 00:00:00

abstract：:The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...

journal_title：Applied biochemistry and biotechnology

authors： Yi X,Luo K,Yang Q,Li XM,Deng WG,Cheng HB,Wang ZL,Zeng GM

更新日期：2013-11-01 00:00:00

abstract：:Non-Systematic Evolution of Ligands by EXponential enrichment (SELEX)selection of aptamers, a novel technology for aptamer selection from libraries of random DNA (or RNA) sequences, involves repetitive steps of partitioning without polymerase chain reaction (PCR) amplification between them. This selection is based on ...

journal_title：Applied biochemistry and biotechnology

authors： Yu X,Yu Y

更新日期：2014-08-01 00:00:00

abstract：:Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

journal_title：Applied biochemistry and biotechnology

authors： Ferreira MS,De Andrade AV,Kennedy JF

更新日期：1991-10-01 00:00:00