Evaluation of the hydrolysis specificity of protease from marine Exiguobacterium sp. SWJS2 via free amino acid analysis.

abstract：:Urease has been immobilized and layered onto the walls of manufactured silicon microchannels. Enzyme immobilization was performed using layer-by-layer nano self-assembly. Alternating layers of oppositely charged polyelectrolytes, with enzyme layers "encased" between them, were deposited onto the walls of the silicon m...

journal_title：Applied biochemistry and biotechnology

authors： Forrest SR,Elmore BB,Palmer JD

更新日期：2005-04-01 00:00:00

abstract：:This study aimed to evaluate the influence of the culture medium supplementation with mineral ions, focusing on the growth of Streptococcus zooepidemicus as well as on the production and average molecular weight (MW) of hyaluronic acid (HA). The ions were investigated in terms of individual absence from the totally su...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Eguchi SY,Santana MH

更新日期：2010-12-01 00:00:00

abstract：:The unique carbohydrate-binding property of lectins makes them invaluable tools in biomedical research. Here, we report the purification, partial primary structure, carbohydrate affinity characterization, crystallization, and preliminary X-ray diffraction analysis of a lactose-specific lectin from Cymbosema roseum see...

journal_title：Applied biochemistry and biotechnology

authors： Rocha BA,Moreno FB,Delatorre P,Souza EP,Marinho ES,Benevides RG,Rustiguel JK,Souza LA,Nagano CS,Debray H,Sampaio AH,de Azevedo WF Jr,Cavada BS

更新日期：2009-03-01 00:00:00

abstract：:Prot-2 protease previously purified to homogeneity from Botrytis cinerea showed potentiality to be used in detergency and for production of bioactive peptides. To extend the characterization of Prot-2 protease, antifungal and antibacterial assays were performed in vitro using protein hydrolysates prepared from muscle ...

journal_title：Applied biochemistry and biotechnology

authors： Abidi F,Aissaoui N,Gaudin JC,Chobert JM,Haertlé T,Marzouki MN

更新日期：2013-05-01 00:00:00

abstract：:Different treatments to improve the thermotolerance of fermenting yeasts for simultaneous ethanol saccharification and fermentation process of cellulosic materials have been examined. Yeasts of the genera Saccharomyces and Kluyveromyces were tested for growth and fermentation at progressively higher temperatures in th...

journal_title：Applied biochemistry and biotechnology

authors： Ballesteros I,Oliva JM,Ballesteros M,Carrasco J

更新日期：1993-04-01 00:00:00

abstract：:A new colorimetric chemosensor for naked-eye detection and determination of cysteine (Cys) based on indicator displacement assay (IDA) was designed using 1-(2-pyridylazo)-2-naphthol (PAN). The indicator exchange occurred between PAN and Cys by the addition of Cys to the Cu(PAN)2 complex, which is accomplished by an im...

journal_title：Applied biochemistry and biotechnology

authors： Deilamy-Rad G,Asghari K,Tavallali H

更新日期：2020-09-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

journal_title：Applied biochemistry and biotechnology

authors： Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

更新日期：2016-08-01 00:00:00

abstract：:Success in generating catalytic antibodies as enzyme mimics lies in the strategic design of the transition-state analog (TSA) for the reaction of interest, and careful development of screening processes for the selection of antibodies that are catalysts. Typically, the choice of TSA structure is straightforward, and t...

journal_title：Applied biochemistry and biotechnology

authors： DeSilva BS,Orosz G,Egodage KL,Carlson RG,Schowen RL,Wilson GS

更新日期：2000-01-01 00:00:00

abstract：:Oxidation of lignin obtained from acetosolv and ethanol/water pulping of sugarcane bagasse was performed by phenol oxidases: tyrosinase (TYR) and laccase (LAC), to increase the number of carbonyl and hydroxyl groups in lignin, and to improve its chelating capacity. The chelating properties of the original and oxidized...

journal_title：Applied biochemistry and biotechnology

authors： Calabria GM,Gonçalves AR

更新日期：2006-04-01 00:00:00

abstract：:One novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfact...

journal_title：Applied biochemistry and biotechnology

authors： Wang Y,Zhang Y,Hu Y

更新日期：2016-11-01 00:00:00

abstract：:The genes encoding purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase), and thymidine phosphorylase (TPase) from Escherichia coli K12 were cloned respectively into expression vector pET-11a or pET-28a. The recombinant plasmids were transformed into the host strain E. coli BL21(DE3) to construct fou...

journal_title：Applied biochemistry and biotechnology

authors： Ge C,OuYang L,Ding Q,Ou L

更新日期：2009-10-01 00:00:00

abstract：:Stachybotrys microspora is a filamentous fungus characterized by the secretion of multiple hydrolytic activities (cellulolytic and non-cellulolytic enzymes). The production of these biocatalysts was studied under submerged culture using glucose, cellulose, and wheat bran as carbon sources. Endoglucanases, pectinases, ...

journal_title：Applied biochemistry and biotechnology

authors： Abdeljalil S,Ben Hmad I,Saibi W,Amouri B,Maalej W,Kaaniche M,Koubaa A,Gargouri A

更新日期：2014-02-01 00:00:00

abstract：:The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical...

journal_title：Applied biochemistry and biotechnology

authors： Gou L,Zhan Y,Lee J,Li X,Lü ZR,Zhou HM,Lu H,Wang XY,Park YD,Yang JM

更新日期：2015-02-01 00:00:00

abstract：:Mesoporous materials possess a hexagonal array of uniform mesopores, high surface areas, and moderate acidity. They are one of the important catalysts in the field of catalytic pyrolysis. In this paper, mesoporous materials of Al-MCM-41, La-Al-MCM-41, and Ce-Al-MCM-41 were synthesized, characterized, and tested as cat...

journal_title：Applied biochemistry and biotechnology

authors： Yu FW,Ji DX,Nie Y,Luo Y,Huang CJ,Ji JB

更新日期：2012-09-01 00:00:00

abstract：:Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then appl...

journal_title：Applied biochemistry and biotechnology

authors： Baloch KA,Upaichit A,Cheirsilp B

更新日期：2021-01-01 00:00:00

abstract：:The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran P,Narayanan GK,Gandhi S,Sethuraman S,Krishnan UM

更新日期：2015-03-01 00:00:00

abstract：:The effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and c...

journal_title：Applied biochemistry and biotechnology

authors： Andrade MM,Barbosa AM,Bofinger MR,Dekker RF,Messias JM,Guedes CL,Zaminelli T,de Oliveira BH,de Lima VM,Dall'antonia LH

更新日期：2013-08-01 00:00:00

abstract：:The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

journal_title：Applied biochemistry and biotechnology

authors： Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

更新日期：2019-10-01 00:00:00

abstract：:A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...

journal_title：Applied biochemistry and biotechnology

authors： Seo KJ,Cho SS,Ppyun HW,Youn MH,Kim SH,Seo BS,Kwon ST

更新日期：2014-07-01 00:00:00

abstract：:Fuel ethanol can be produced from softwood through hydrolysis in an enzymatic process. Prior to enzymatic hydrolysis of the softwood, pretreatment is necessary. In this study, two-step steam pretreatment employing dilute H2SO4 impregnation in the first step and SO2 impregnation in the second step, to improve the overa...

journal_title：Applied biochemistry and biotechnology

authors： Söderström J,Pilcher L,Galbe M,Zacchi G

更新日期：2003-04-01 00:00:00

abstract：:This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was perform...

journal_title：Applied biochemistry and biotechnology

authors： Poppe JK,Matte CR,Fernandez-Lafuente R,Rodrigues RC,Ayub MAZ

更新日期：2018-11-01 00:00:00

abstract：:The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently o...

journal_title：Applied biochemistry and biotechnology

authors： Muthuraman P

更新日期：2014-06-01 00:00:00

abstract：:Marine Aspergillus awamori BTMFW032, recently reported by us, produce acidophilic tannase as extracellular enzyme. Here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the simultaneous production of gallic ac...

journal_title：Applied biochemistry and biotechnology

authors： Beena PS,Basheer SM,Bhat SG,Bahkali AH,Chandrasekaran M

更新日期：2011-07-01 00:00:00

abstract：:Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

journal_title：Applied biochemistry and biotechnology

authors： Kim M,Aita G,Day DF

更新日期：2010-05-01 00:00:00

abstract：:Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galac...

journal_title：Applied biochemistry and biotechnology

authors： Koroljova OV,Stepanova EV,Gavrilova VP,Biniukov VI,Jaropolov AI,Varfolomeyev SD,Scheller F,Makower A,Otto A

更新日期：1999-02-01 00:00:00

abstract：:We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35degreesC. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the so...

journal_title：Applied biochemistry and biotechnology

authors： Huang WC,Ramey DE,Yang ST

更新日期：2004-04-01 00:00:00

abstract：:Soybean hulls were subjected to thermo-mechanical extrusion pretreatment at various in-barrel moisture contents and screw speeds. Extrusion degraded the lignocellulosic structure and enhanced enzymatic hydrolysis of soybean hulls, with up to 155% increase in glucose yield as compared to untreated substrate. Greater gl...

journal_title：Applied biochemistry and biotechnology

authors： Yoo J,Alavi S,Vadlani P,Behnke KC

更新日期：2012-02-01 00:00:00

abstract：:Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

journal_title：Applied biochemistry and biotechnology

authors： Yan J,Gui X,Wang G,Yan Y

更新日期：2012-02-01 00:00:00

abstract：:The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit of NHase. Since the C-domain could be present as a naturally separate domain in a few NHases, the whole C-doma...

journal_title：Applied biochemistry and biotechnology

authors： Sun W,Zhu L,Chen X,Wu L,Zhou Z,Liu Y

更新日期：2016-04-01 00:00:00