Abstract：
:Non-Systematic Evolution of Ligands by EXponential enrichment (SELEX)selection of aptamers, a novel technology for aptamer selection from libraries of random DNA (or RNA) sequences, involves repetitive steps of partitioning without polymerase chain reaction (PCR) amplification between them. This selection is based on non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) and has exceptionally high efficiency. In this paper, a mathematical analysis was carried out to predict the levels of enrichment of non-SELEX selection under different conditions such as different protein concentrations and different efficiencies of partitioning. Investigated results suggest that the magnitude of the bulk affinity (k d) being 10(4) or 10(5) μM for the initial pool has no obvious effect on selective enrichment and that the first, second, and third rounds of non-SELEX selection have different optimum protein concentration values [T f] that give maximum enrichment levels when [T f] ranges from 0.0005 to 0.5 μM. The significance of analyzing selective enrichment of NECEEM-based non-SELEX with the efficiency of partitioning target-bound ligands from free ligands has been demonstrated.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Yu X,Yu Ydoi
10.1007/s12010-014-0989-9subject
Has Abstractpub_date
2014-08-01 00:00:00pages
2019-27issue
8eissn
0273-2289issn
1559-0291journal_volume
173pub_type
杂志文章abstract：:Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...
journal_title：Applied biochemistry and biotechnology
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journal_title：Applied biochemistry and biotechnology
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更新日期：2004-04-01 00:00:00
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更新日期：2008-04-01 00:00:00
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更新日期：2000-05-01 00:00:00
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更新日期：2016-03-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
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更新日期：2010-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-012-9861-y
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