Abstract:
:Strain DN002 isolated from petroleum-contaminated soil was identified as Achromobacter xylosoxidans based on morphological and biochemical properties and 16S rRNA phylogeny, and investigated for its potential to utilize numerous polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene and pyrene as sole carbon and energy resource. Biodegradation studies showed that 500 mg(·)l(-1)fluranthene was degraded to 35.6 ± 0.3 mg(·)l(-1) by DN002 after 14 days incubation. During fluoranthene biodegradation, catechol 2,3 dioxygenase (C23O) activity was augmented 1.5 times more than catechol 1,2 dioxygenase (C12O), which indicated that C23O played a major role in fluoranthene degradation by DN002. Protein profiles were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional electrophoresis then analyzed by mass spectrometry induced by fluoranthene; a molecular mass range of 18 ∼ 66 kDa proteins were found upregulated compared with the uninduced control sample, including multiple isoenzymes of β-oxidation and dehydrogenases as well as dioxygenases. Besides, some new proteins, i.e., dihydrolipoamide succinyltransferase and aldehyde dehydrogenase family proteins and isocitrate lyase were also synthesized.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Ma YL,Lu W,Wan LL,Luo Ndoi
10.1007/s12010-014-1347-7subject
Has Abstractpub_date
2015-02-01 00:00:00pages
1294-305issue
3eissn
0273-2289issn
1559-0291journal_volume
175pub_type
杂志文章abstract::Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-009-8728-3
更新日期:2010-04-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-008-8214-3
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journal_title:Applied biochemistry and biotechnology
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abstract::The semi-continuous anaerobic digestion of food waste was investigated in 1-L and 20-L continuously stirred tank reactors (CSTRs), to identify the optimum operation condition and the methane production of the semi-continuous anaerobic process. Results from a 1-L digester indicated that the optimum organic loading rate...
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pub_type: 杂志文章
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journal_title:Applied biochemistry and biotechnology
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abstract::Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...
journal_title:Applied biochemistry and biotechnology
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doi:10.1007/s12010-011-9380-2
更新日期:2011-12-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03347-1
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abstract::Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...
journal_title:Applied biochemistry and biotechnology
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doi:10.1007/s12010-012-9788-3
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abstract::Microbial lipid is a promising source of oil to produce biofuel if it can be generated from lignocellulosic materials. Mortierella isabellina is a filamentous fungal species featuring high content of oil in its cell biomass. In this work, M. isabellina was studied for lipid production from corn stover. The experimenta...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1117-6
更新日期:2014-09-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::Serratia marcescens SP511 was previously reported to be an L-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L of L-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02916452
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-0987-y
更新日期:2014-08-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02920423
更新日期:1997-04-01 00:00:00
abstract::Studying biofilm formation and influence of the matrix composition was heavy because only old and long methods were employed up to now: confocal microscopy, fluorescent chemical markers, and/or dying techniques. In this context, an innovative tool, the BioFilm Ring Test, was here employed to explore the role of exopol...
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doi:10.1007/s12010-008-8199-y
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:128:3:195
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doi:10.1007/s12010-007-8102-2
更新日期:2008-03-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1385/abab:119:1:79
更新日期:2004-10-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9591-1
更新日期:2012-07-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02729071
更新日期:2007-05-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0116-3
更新日期:2013-04-01 00:00:00