Screening thermotolerant white-rot fungi for decolorization of wastewaters.

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

更新日期：1992-09-01 00:00:00

abstract：:The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...

journal_title：Applied biochemistry and biotechnology

authors： Errasti ME,Natalucci CL,Caffini NO,Rotelli AE,Brullo A,Maras B,Trejo SA,López LMI

更新日期：2018-09-01 00:00:00

abstract：:For trial use in the local chemotherapy of cancer by a combination of cytosine deaminase (EC 3.5.4.1) and 5-fluorocytosine (J. Biotechnol., (1985), 2, 13-21), 40 U of partially purified cytosine deaminase was obtained from 500 g of commercial compressed bakers' yeast. The enzyme, which is unstable, was immobilized to ...

journal_title：Applied biochemistry and biotechnology

authors： Katsuragi T,Sakai T,Tonomura K

更新日期：1987-09-01 00:00:00

abstract：:Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

journal_title：Applied biochemistry and biotechnology

authors： Xia C,Wei W,Hu B

更新日期：2014-04-01 00:00:00

abstract：:In biotechnological processes, a great number of factors can influence the income productivity and conversion. Normally, it is not evident which of these factors are the most important and how they interact. In this work, multivariate analysis techniques are used as experimental design coupled to a detailed determinis...

journal_title：Applied biochemistry and biotechnology

authors： Lunelli BH,Rivera EC,Vasco de Toledo EC,Wolf Maciel MR,Maciel Filho R

更新日期：2008-03-01 00:00:00

abstract：:We have described a novel red biochrome, 514 Da in size, produced by solid-state cultivation of a bacterial isolate obtained from garden soil. The growth requirements of the isolate, the chemical characteristics of the biochrome produced, and the application of the biochrome in dying of silk, wool, and cotton fabrics ...

journal_title：Applied biochemistry and biotechnology

authors： Vaidyanathan J,Bhathena-Langdana Z,Adivarekar RV,Nerurkar M

更新日期：2012-01-01 00:00:00

abstract：:Urease has been immobilized and layered onto the walls of manufactured silicon microchannels. Enzyme immobilization was performed using layer-by-layer nano self-assembly. Alternating layers of oppositely charged polyelectrolytes, with enzyme layers "encased" between them, were deposited onto the walls of the silicon m...

journal_title：Applied biochemistry and biotechnology

authors： Forrest SR,Elmore BB,Palmer JD

更新日期：2005-04-01 00:00:00

abstract：:The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed o...

journal_title：Applied biochemistry and biotechnology

authors： Murhammer DW

更新日期：1991-12-01 00:00:00

abstract：:Lupulones, naturally produced by glandular trichomes of hop (Humulus lupulus), are prenylated phloroglucinol derivatives that contribute the bitter flavor of beer and demonstrate antimicrobial and anticancer activities. It is appealing to develop microbial cell factories such that lupulones may be produced via ferment...

journal_title：Applied biochemistry and biotechnology

authors： Guo X,Shen H,Liu Y,Wang Q,Wang X,Peng C,Liu W,Zhao ZK

更新日期：2019-07-01 00:00:00

abstract：:The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochro...

journal_title：Applied biochemistry and biotechnology

authors： Yu H,Liu J,Li J,Zang T,Luo G,Shen J

更新日期：2005-11-01 00:00:00

abstract：:The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synt...

journal_title：Applied biochemistry and biotechnology

authors： Rathod JP,Vira C,Lali AM,Prakash G

更新日期：2020-04-01 00:00:00

abstract：:Further characterization of an aspartyl protease from Mucor bacilliformis with milk-clotting activity was performed. An extinction coefficient, epsilon 278 cm = 1.61 mL/mg/cm, a molecular mass of 35,400 Da and a pI of 5.2 were determined. Proteolytic activity and kinetic parameters were evaluated by using the hexapept...

journal_title：Applied biochemistry and biotechnology

authors： Venera GD,Machalinski C,Zumárraga H,Biscoglio de Jiménez Bonino MJ

更新日期：1997-12-01 00:00:00

abstract：:Equipment and procedures were developed to study the conversion of lignocellulosic biomass to carboxylic acids using high-solids countercurrent fermentations. Countercurrent fermentations of cattle manure yielded a rapid fermentation (maximum 2.98 g of total acid/[L x d]) with high acid concentrations (maximum of 32.5...

journal_title：Applied biochemistry and biotechnology

authors： Ross MK,Holtzapple MT

更新日期：2001-05-01 00:00:00

abstract：:Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

journal_title：Applied biochemistry and biotechnology

authors： Nghiem NP,Cofer TM

更新日期：2007-05-01 00:00:00

abstract：:To investigate the production of cellulases and xylanases from Penicillium echinulatum 9A02S1, solid-state fermentation (SSF) was performed by using different ratios of sugar cane bagasse (SCB) and wheat bran (WB). The greatest filter paper activity obtained was 45.82 ± 1.88 U gdm(-1) in a culture containing 6SCB/4WB ...

journal_title：Applied biochemistry and biotechnology

authors： Camassola M,Dillon AJ

更新日期：2010-11-01 00:00:00

abstract：:We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of tyrosinase. TFE increased the degree of secondary structure of tyrosinase, which directly resulted in enzyme inactivation. A reciprocal study showed that TFE inhibited tyrosinase in a slope-parabolic mixed-type inhibition m...

journal_title：Applied biochemistry and biotechnology

authors： Lü ZR,Shi L,Wang J,Park D,Bhak J,Yang JM,Park YD,Zhou HW,Zou F

更新日期：2010-04-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:Scheffersomyces stipitis was cultivated in an optimized, controlled fed-batch fermentation for production of ethanol from glucose-xylose mixture. Effect of feed medium composition was investigated on sugar utilization and ethanol production. Studying influence of specific cell growth rate on ethanol fermentation perfo...

journal_title：Applied biochemistry and biotechnology

authors： Unrean P,Nguyen NH

更新日期：2013-03-01 00:00:00

abstract：:Pretreatment-induced structural alteration is critical in influencing the rate and extent of enzymatic saccharification of lignocellulosic biomass. The present work has investigated structural features of rice straw pretreated by hot-compressed water (HCW) from 140 to 240 °C for 10 or 30 min and enzymatic hydrolysis p...

journal_title：Applied biochemistry and biotechnology

authors： Yu G,Yano S,Inoue H,Inoue S,Wang J,Endo T

更新日期：2014-11-01 00:00:00

abstract：:For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...

journal_title：Applied biochemistry and biotechnology

authors： Zhao L,Jiang Y,Fang H,Zhang H,Cheng S,Rajoka MSR,Wu Y

更新日期：2019-10-01 00:00:00

abstract：:The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obta...

journal_title：Applied biochemistry and biotechnology

authors： Manni L,Jellouli K,Ghorbel-Bellaaj O,Agrebi R,Haddar A,Sellami-Kamoun A,Nasri M

更新日期：2010-04-01 00:00:00

abstract：:A study was undertaken to examine the influence of biogenic nanoparticles synthesized from Tridax procumbens on different parameters of seed germination, seedling growth, and various biochemical parameters in four Eruca sativa varieties having low percentage of germination. Seeds were treated with different concentrat...

journal_title：Applied biochemistry and biotechnology

authors： Ushahra J,Bhati-Kushwaha H,Malik CP

更新日期：2014-09-01 00:00:00

abstract：:Cis-epoxysuccinic acid hydrolase (CESH) is an enzyme that catalyzes cis-epoxysuccinic acid to produce enantiomeric L(+)-tartaric acid. The production of tartaric acid by using CESH would be valuable in the chemical industry because of its high yield and selectivity, but the low stability of CESH hampers its applicatio...

journal_title：Applied biochemistry and biotechnology

authors： Wang S,Cui GZ,Song XF,Feng Y,Cui Q

更新日期：2012-10-01 00:00:00

abstract：:Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, ...

journal_title：Applied biochemistry and biotechnology

authors： Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

更新日期：2011-12-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:Candida boidinii produces significant amounts of xylitol from xylose, and assays of crude homogenates for aldose (xylose) reductase (XYL1p) have been reported to show relatively high activity with NADH as a cofactor even though XYL1p purified from this yeast does not have such activity. A gene coding for XYL1p from C....

journal_title：Applied biochemistry and biotechnology

authors： Kang MH,Ni H,Jeffries TW

更新日期：2003-04-01 00:00:00

abstract：:Serratia marcescens SP511 was previously reported to be an L-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L of L-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions...

journal_title：Applied biochemistry and biotechnology

authors： Masuda M,Takamatu S,Nishimura N,Komatsubara S,Tosa T

更新日期：1993-12-01 00:00:00

abstract：:Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

journal_title：Applied biochemistry and biotechnology

authors： Aldin S,Tu F,Nakhla G,Ray MB

更新日期：2011-08-01 00:00:00