Increased xylose reductase activity in the xylose-fermenting yeast Pichia stipitis by overexpression of XYL1.

abstract：:More than two decades ago, a group of research engineers and applied scientists with interest in energy applications of bioprocessing initiated an annual symposium series ultimately entitled "Biotechnology for Fuels and Chemicals." The Department of Energy, several of the national laboratories, and various industrial ...

journal_title：Applied biochemistry and biotechnology

authors： Scott CD

更新日期：2004-04-01 00:00:00

abstract：:The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best...

journal_title：Applied biochemistry and biotechnology

authors： Bernardes OL,Bevilaqua JV,Leal MC,Freire DM,Langone MA

更新日期：2007-04-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:This study was carried out to evaluate the capacity of anaerobic granular sludge for oxidizing ammonium and p-cresol with nitrate as terminal electron acceptor. Kinetics for the anaerobic oxidation of ammonium and p-cresol is described in this paper. The phenolic compound was very efficiently consumed, achieving 65 % ...

journal_title：Applied biochemistry and biotechnology

authors： González-Blanco G,Cervantes FJ,Beristain-Cardoso R,Gómez J

更新日期：2015-08-01 00:00:00

abstract：:Xanthomonas campestris w.t. was used for production of xanthan gum in fermentations with chestnut flour for the first time. Fermentations were carried out with either chestnut flour or its soluble sugars (33.5%) and starch (53.6%), respectively, at 28 degrees C and 200 rpm at initial pH 7.0 in flasks. The effect of ag...

journal_title：Applied biochemistry and biotechnology

authors： Liakopoulou-Kyriakides M,Psomas SK,Kyriakidis DA

更新日期：1999-12-01 00:00:00

abstract：:Respiratory-deficient mutants of Rhizopus oryzae (R. oryzae) AS 3.3461 were acquired by ultraviolet (UV) irradiation to investigate changes in intracellular NADH metabolic pathway and its influence on the fermentation characteristics of the strain. Compared with R. oryzae AS 3.3461, the intracellular ATP level of the ...

journal_title：Applied biochemistry and biotechnology

authors： Shu C,Guo C,Luo S,Jiang S,Zheng Z

更新日期：2015-08-01 00:00:00

abstract：:Palm kernel press cake (PKC) is a residue from palm oil extraction presently only used as a low protein feed supplement. PKC contains 50% fermentable hexose sugars present in the form of glucan and mainly galactomannan. This makes PKC an interesting feedstock for processing into bioethanol or in other biorefinery proc...

journal_title：Applied biochemistry and biotechnology

authors： Jørgensen H,Sanadi AR,Felby C,Lange NE,Fischer M,Ernst S

更新日期：2010-05-01 00:00:00

abstract：:The enzyme cellobiase from Novo was immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67 and 13.7%, respectively. The activity of the free enzyme (FE) and immobilized enzyme (IE) was determined with 2 g/L of cellobiose, from 40...

journal_title：Applied biochemistry and biotechnology

authors： Calsavara LP,De Moraes FF,Zanin GM

更新日期：2001-04-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00

abstract：:The rhg gene encoding a rhamnogalacturonase was isolated from the novel strain A1 of Aspergillus niger. It consists of an ORF of 1.505 kb encoding a putative protein of 446 amino acids with a predicted molecular mass of 47 kDa, belonging to the family 28 of glycosyl hydrolases. The nature and position of amino acids c...

journal_title：Applied biochemistry and biotechnology

authors： Damak N,Abdeljalil S,Taeib NH,Gargouri A

更新日期：2015-08-01 00:00:00

abstract：:In this study, benzene, toluene, and xylenes (BTX) removal from contaminated water by physical, chemical, and biological processes was studied. Results showed that air sparging in polluted water can reduce monoaromatic compounds from 140,000 to about 5 microg/l in only 1 h process with a gassing rate of 0.33 VVM. This...

journal_title：Applied biochemistry and biotechnology

authors： Farhadian M,Duchez D,Vachelard C,Larroche C

更新日期：2008-12-01 00:00:00

abstract：:Alpha-lactalbumin hydrolysate is of significant interest, due to its potential application as a source of bioactive peptides in nutraceutical and pharmaceutical domains. This study was focused on the cholecystokinin (CCK) family compounds which are small peptides involved in the satiety control. The action of chymotry...

journal_title：Applied biochemistry and biotechnology

authors： Catiau L,Delval-Dubois V,Guillochon D,Nedjar-Arroume N

更新日期：2011-11-01 00:00:00

abstract：:A DNA fragment of 2,042 bp containing a novel beta-mannanase gene, man5A, was identified from the genome of the mannan-degrading bacterium Bacillus circulans CGMCC1554. The open reading frame of man5A comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kDa. The amino acid seq...

journal_title：Applied biochemistry and biotechnology

authors： Yang P,Li Y,Wang Y,Meng K,Luo H,Yuan T,Bai Y,Zhan Z,Yao B

更新日期：2009-10-01 00:00:00

abstract：:The effect of temperature on the performance of an anaerobic sequencing biofilm batch reactor (ASBBR) with liquid-phase recirculation was assessed. Assays were performed using a recirculation velocity of 0.20 cm/s, 8-h cycles, and an average treated synthetic wastewater volume of 2 L/cycle with a concentration of 500 ...

journal_title：Applied biochemistry and biotechnology

authors： Agibert SA,Moreira MB,Ratusznei SM,Rodrigues JA,Zaiat M,Foresti E

更新日期：2007-02-01 00:00:00

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of usi...

journal_title：Applied biochemistry and biotechnology

authors： Niyonzima FN,More SS

更新日期：2014-10-01 00:00:00

abstract：:The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect ...

journal_title：Applied biochemistry and biotechnology

authors： Richardson PJ,Luzio JP

更新日期：1986-10-01 00:00:00

abstract：:A unicellular marine green alga, Chlamydomonas perigranulata, was demonstrated to synthesize starch through photosynthesis, store it in a cell, and ferment it under anaerobic conditions in the dark to produce ethanol, 2,3-butanediol (butanediol), acetic acid, and carbon dioxide (CO2). Previous fermentation data of an ...

journal_title：Applied biochemistry and biotechnology

authors： Hon-Nami K

更新日期：2006-04-01 00:00:00

abstract：:This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

journal_title：Applied biochemistry and biotechnology

authors： Sun ZL,Sun LQ,Chen GZ

更新日期：2019-04-01 00:00:00

abstract：:To facilitate structural studies of receptor-interacting protein 3 (RIP3), we developed a large-scale expression system of a glutathione-S-transferase (GST) fused with an 82 amino acid RIP3 protein in Escherichia coli. RIP3 truncation was subcloned into the pGEX-4T-1 vector and overexpressed in BL21(DE3)RIL cells. The...

journal_title：Applied biochemistry and biotechnology

authors： Jeong MS,Park JS,Jang SB

更新日期：2007-05-01 00:00:00

abstract：:Glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase is an enzyme that converts GL-7-ACA to 7-aminocephalosporanic acid, a starting material for semisynthetic cephalosporin antibiotics. In this study, optimal conditions for the immobilization of GL-7-ACA acylase were determined by experimental observations and stat...

journal_title：Applied biochemistry and biotechnology

authors： Park SW,Lee JW,Hong SI,Kim SW

更新日期：2003-03-01 00:00:00

abstract：:Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

journal_title：Applied biochemistry and biotechnology

authors： Bresolin ITL,Bresolin IRAP,Bueno SMA

更新日期：2020-06-01 00:00:00

abstract：:Sugarcane bagasse samples were pretreated with ozone via atmospheric O2 pressure plasma. A delignification efficiency of approximately 80 % was observed within 6 h of treatment. Some hemicelluloses were removed, and the cellulose was not affected by ozonolysis. The quantity of moisture in the bagasse had a large influ...

journal_title：Applied biochemistry and biotechnology

authors： Souza-Corrêa JA,Oliveira C,Wolf LD,Nascimento VM,Rocha GJ,Amorim J

更新日期：2013-09-01 00:00:00

abstract：:A comparative study was carried out to evaluate protease production in solid-state fermentation (SSF) and submerged fermentation (SmF) by nine different thermophilic fungi--Thermoascus aurantiacus Miehe, Thermomyces lanuginosus, T. lanuginosus TO.03, Aspergillus flavus 1.2, Aspergillus sp. 13.33, Aspergillus sp. 13.34...

journal_title：Applied biochemistry and biotechnology

authors： Macchione MM,Merheb CW,Gomes E,da Silva R

更新日期：2008-03-01 00:00:00

abstract：:In order to detect low levels of enzyme activity, specifically glucose oxidase, in biological samples, an immunoenzymatic assay was developed since currently available methods could not be used because of either their lack of sensitivity or the conditions prevailing in our samples: turbidity of the medium, presence of...

journal_title：Applied biochemistry and biotechnology

authors： de Bellefontaine C,Josse F,Domurado M,Domurado D

更新日期：1994-08-01 00:00:00

abstract：:Strain DN002 isolated from petroleum-contaminated soil was identified as Achromobacter xylosoxidans based on morphological and biochemical properties and 16S rRNA phylogeny, and investigated for its potential to utilize numerous polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene and pyrene as sole carbon and...

journal_title：Applied biochemistry and biotechnology

authors： Ma YL,Lu W,Wan LL,Luo N

更新日期：2015-02-01 00:00:00

abstract：:This work aimed to characterize two native microalgal strains newly isolated from South Mediterranean areas and identified as Chlorella sorokiniana ES3 and Neochloris sp. AM2. The growth properties and biochemical composition of these microalgae were evaluated in different culture media (Algal, BG-11, f/2, and Conway)...

journal_title：Applied biochemistry and biotechnology

authors： Jazzar S,Berrejeb N,Messaoud C,Marzouki MN,Smaali I

更新日期：2016-08-01 00:00:00

abstract：:The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is descri...

journal_title：Applied biochemistry and biotechnology

authors： Das SP,Ravindran R,Ahmed S,Das D,Goyal D,Fontes CM,Goyal A

更新日期：2012-07-01 00:00:00