Immunoaffinity purification of subcellular particles and organelles.

abstract：:The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal i...

journal_title：Applied biochemistry and biotechnology

authors： Mulay YR,Deopurkar RL

更新日期：2018-02-01 00:00:00

abstract：:The 1,3-dihydroxyacetone (DHA)-overproducing mutant of Gluconobacter oxydans was screened via UV mutagenesis to enhance the DHA production, and the DHA fermentation condition was optimized using the dissolved oxygen (DO) control strategy. The stable mutant G. oxydans ZJB11001 exhibits high DHA productivity and can tol...

journal_title：Applied biochemistry and biotechnology

authors： Hu ZC,Zheng YG

更新日期：2011-11-01 00:00:00

abstract：:Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...

journal_title：Applied biochemistry and biotechnology

authors： Zhang B,Dong C,Shang Q,Cong Y,Kong W,Li P

更新日期：2013-12-01 00:00:00

abstract：:Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Four...

journal_title：Applied biochemistry and biotechnology

authors： Singh S,Singh AN,Verma A,Dubey VK

更新日期：2013-12-01 00:00:00

abstract：:The merit of deacetylation of corn stover prior to pretreatment is decreasing the formation of inhibitors and improving enzyme hydrolysis, proved in dilute acid pretreatment. However, few studies are done on how deacetylation would affect bioconversion process containing steam explosion. In this study, the effect of d...

journal_title：Applied biochemistry and biotechnology

authors： Tang Y,Dou X,Hu J,Jiang J,Saddler JN

更新日期：2018-01-01 00:00:00

abstract：:A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. ...

journal_title：Applied biochemistry and biotechnology

authors： Puri N,Niazi A,Srivastava AK,Rajesh

更新日期：2014-10-01 00:00:00

abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...

journal_title：Applied biochemistry and biotechnology

authors： Chet I,Inbar J

更新日期：1994-07-01 00:00:00

abstract：:The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

journal_title：Applied biochemistry and biotechnology

authors： Osa T

更新日期：1993-04-01 00:00:00

abstract：:The presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. In the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-p...

journal_title：Applied biochemistry and biotechnology

authors： Daverey A,Pakshirajan K

更新日期：2011-03-01 00:00:00

abstract：:Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research. Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transfo...

journal_title：Applied biochemistry and biotechnology

authors： Dang X,Shan Z,Chen H

更新日期：2016-11-01 00:00:00

abstract：:The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization of D-mannose and D-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation by Pseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to levels three- to four...

journal_title：Applied biochemistry and biotechnology

authors： Allenza P,Morrell MJ,Detroy RW

更新日期：1990-04-01 00:00:00

abstract：:Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

journal_title：Applied biochemistry and biotechnology

authors： Kim M,Aita G,Day DF

更新日期：2010-05-01 00:00:00

abstract：:Polysaccharide glycosyl hydrolases are a group of enzymes that hydrolyze the glycosidic bond between carbohydrates or between a carbohydrate and a noncarbohydrate moiety. Here we illustrate that traditional schemes for grouping enzymes, such as by substrate specificity or by organism of origin, are not appropriate whe...

journal_title：Applied biochemistry and biotechnology

authors： Himmel ME,Karplus PA,Sakon J,Adney WS,Baker JO,Thomas SR

更新日期：1997-04-01 00:00:00

abstract：:Ornithine carbamoyltransferase folding/unfolding is a complex and not completely understood process. Our experimental results suggest that ornithine carbamoyltransferase interacts in a completely different way with urea and guanidine hydrochloride. In fact, we noticed that, increasing concentration from 0.0 to 8.0 M o...

journal_title：Applied biochemistry and biotechnology

authors： Barreca D,Laganà G,Ficarra S,Tellone E,Leuzzi U,Galtieri A,Bellocco E

更新日期：2014-01-01 00:00:00

abstract：:A novel immobilized-enzyme system that has glucoamylase on the surface of, and glucose oxidase within, polyurea microcapsules was developed. This system was found to carry out its sequential enzymatic reaction effectively. It was also demonstrated that the use of microcapsules was useful for this immobilized-enzyme sy...

journal_title：Applied biochemistry and biotechnology

authors： Komori T,Muramatsu N,Kondo T

更新日期：1987-02-01 00:00:00

abstract：:Proteases constitute one of the most important groups of industrial enzymes, accounting for at least 25% of the total enzyme sales, with two-thirds of the proteases produced commercially being of microbial origin. Immobilized enzymes are currently the subject of considerable interest because of their advantages over s...

journal_title：Applied biochemistry and biotechnology

authors： Kirkkopru I,Alpaslan C,Omay D,Güvenilir Y

更新日期：2006-04-01 00:00:00

abstract：:Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...

journal_title：Applied biochemistry and biotechnology

authors： Kasra-Kermanshahi R,Tajer-Mohammad-Ghazvini P,Bahrami-Bavani M

更新日期：2020-11-17 00:00:00

abstract：:Besides existing models of chemical or biotechnological origin for hemoproteins like peroxidases and cytochromes P450, catalytic antibodies (Abs) with a metalloporphyrin cofactor represent a promising alternative route to catalysts tailored for selective oxidation reactions. A brief overview of the literature shows th...

journal_title：Applied biochemistry and biotechnology

authors： Mahy JP,Desfosses B,de Lauzon S,Quilez R,Desfosses B,Lion L,Mansuy D

更新日期：1998-10-01 00:00:00

abstract：:Thermomucor indicae-seudaticae, a glucoamylase-producing thermophilic mould, was mutagenised using nitrous acid and gamma ((60)Co) irradiation in a sequential manner to isolate deregulated mutants for enhanced production of glucoamylase. The mutants were isolated on Emerson YpSs agar containing a non-metabolisable glu...

journal_title：Applied biochemistry and biotechnology

authors： Kumar P,Satyanarayana T

更新日期：2009-07-01 00:00:00

abstract：:Nanotechnology is an emerging technology which is an amalgamation of different aspects of science and technology that includes disciplines such as electrical engineering, mechanical engineering, biology, physics, chemistry, and material science. It has potential in the fields of information and communication technolog...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty M,Jain S,Rani V

更新日期：2011-11-01 00:00:00

abstract：:Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

journal_title：Applied biochemistry and biotechnology

authors： Rodrigues DC,Silva SS,Prata AM,Felipe MG

更新日期：1998-04-01 00:00:00

abstract：:Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, star...

journal_title：Applied biochemistry and biotechnology

authors： Nampoothiri KM,Tomes GJ,Roopesh K,Szakacs G,Nagy V,Soccol CR,Pandey A

更新日期：2004-07-01 00:00:00

abstract：:Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to for...

journal_title：Applied biochemistry and biotechnology

authors： Bhuvana M,Dharuman V

更新日期：2014-10-01 00:00:00

abstract：:Enzymatic polymerization of phenolic compounds (catechol, resorcinol, and hydroquinone) was carried out using laccase. The mechanism of polymerization and the structures of the polymers were evaluated in terms of UV-Vis and Fourier transform infrared spectroscopy. The molecular weights of the produced polyphenols were...

journal_title：Applied biochemistry and biotechnology

authors： Sun X,Bai R,Zhang Y,Wang Q,Fan X,Yuan J,Cui L,Wang P

更新日期：2013-12-01 00:00:00

abstract：:The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electroc...

journal_title：Applied biochemistry and biotechnology

authors： Singh S,Kaushal A,Khare S,Kumar A

更新日期：2014-05-01 00:00:00

abstract：:An Aspergillus terreus, isolated from oil contaminated soil, could degrade a wide range of petroleum hydrocarbons including the immediate oxidation products of hydrocarbons, like alkanols and alkanals. Among all the linear chain carbon substrates, highest growth of 39.1 + or - 3.8 g l(-1) (wet weight) was observed whe...

journal_title：Applied biochemistry and biotechnology

authors： Kumar AK,Vatsyayan P,Goswami P

更新日期：2010-03-01 00:00:00

abstract：:The feasibility of using mixtures of C4 and C5 chain-length aliphatic alcohols from fusel oil as the bulk organic media for lipase-mediated synthesis of laurate esters was assessed. Reaction mixtures consisted of lauric acid, lipase, solvent (if added), and appropriate amount of fusel oil (previously dehydrated with i...

journal_title：Applied biochemistry and biotechnology

authors： De Castro HF,Moriya RY,Oliveira PC,Soares CM

更新日期：1999-04-01 00:00:00

abstract：:In this study, immobilization conditions and bioethanol production characteristics of immobilized Saccharomyces bayanus were investigated into sodium alginate-graft-poly(N-vinyl-2-pyrrolidone; NaAlg-g-PVP) matrix. The matrix that crosslinked with calcium clorid was used for immobilization of S. bayanus. Bioethanol pro...

journal_title：Applied biochemistry and biotechnology

authors： İnal M,Yiğitoğlu M

更新日期：2012-09-01 00:00:00

abstract：:Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...

journal_title：Applied biochemistry and biotechnology

authors： Khanna S,Srivastava AK

更新日期：2009-05-01 00:00:00

abstract：:We have previously demonstrated that pretreatment of corn stover with dilute sulfuric acid can achieve high digestibility and efficient recovery of hemicellulose sugars with high yield and concentration. Further improvement of this process was sought in this work. A modification was made in the operation of the percol...

journal_title：Applied biochemistry and biotechnology

authors： Zhu Y,Lee YY,Elander RT

更新日期：2005-04-01 00:00:00