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Mutagenesis for Improvement of Activity and Stability of Prolyl Aminopeptidase from Aspergillus oryzae.

Abstract：

:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-prone polymerase chain reaction, and nonconservative amino acids within 3 Å of the substrate L-proline-p-nitroaniline were selected as site-directed saturation mutagenesis sites via homologous simulation and molecular docking of AoPAP. Variants featuring high catalytic efficiency were screened by a high-throughput screening method. The specific activities of the variants of 3D9, C185V, and Y393W were 127 U mg-1, 156 U mg-1, and 120 U mg-1, respectively, which were 27%, 56%, and 20% higher than those of the wild type, with a value of 100 U mg-1. The half-life of thermostability of the mutant 3D9 was 4.5 h longer than that of the wild type at 50 °C. The mutant C185V improved thermostability and had a half-life 2 h longer than that of the wild type at a pH of 6.5. Prolyl aminopeptidase had improved stability within the acidic range and thermostability after modification, making it more suitable for a synergistic combination with various acidic and neutral endoproteases.

journal_name

Appl Biochem Biotechnol

journal_title

Applied biochemistry and biotechnology

authors

Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

doi

10.1007/s12010-020-03277-y

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

1483-1498

issue

4

eissn

0273-2289

issn

1559-0291

pii

10.1007/s12010-020-03277-y

journal_volume

191

pub_type

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